Physical Interpretation of the Maximum Receptor−Ligand Bond Spacing to Ensure Cell Adhesion in Ligand-Coated Substrates

Langmuir ◽  
2008 ◽  
Vol 24 (11) ◽  
pp. 5644-5646 ◽  
Author(s):  
YuJie Wei
Keyword(s):  
Cell Adhesion ◽  
Physical Interpretation ◽  
Receptor Ligand ◽  
Ligand Bond

scholarly journals Segregation of receptor–ligand complexes in cell adhesion zones: phase diagrams and the role of thermal membrane roughness

2010 ◽  
Vol 12 (9) ◽  
pp. 095003 ◽  
Author(s):  
B Różycki ◽  
R Lipowsky ◽  
T R Weikl
Keyword(s):  
Cell Adhesion ◽  
Phase Diagrams ◽  
Receptor Ligand ◽  
Membrane Roughness

scholarly journals Distortion of Protein Receptor Decreases the Lifetime of Receptor-ligand Bond

2009 ◽  
Vol 96 (3) ◽  
pp. 288a
Author(s):  
Senli Guo
Keyword(s):  
Receptor Ligand ◽  
Protein Receptor ◽  
Ligand Bond

scholarly journals Quantifying Molecular Forces with Serially Connected Force Sensors

2018 ◽  
Author(s):  
Y. Murad ◽  
I. T.S. Li
Keyword(s):  
Cell Adhesion ◽  
Dynamic Range ◽  
Computational Study ◽  
Experimental Studies ◽  
High Signal ◽  
Force Sensors ◽  
Adhesion Forces ◽  
Receptor Ligand ◽  
Sequence Composition ◽  
Molecular Force

AbstractTo understand the mechanical forces involved in cell adhesion, molecular force sensors have been developed to study tension through adhesion proteins. Recently, a class of molecular force sensors called tension gauge tether (TGT) have been developed that rely on irreversible force-dependent dissociation of DNA duplex to study cell adhesion forces. While the TGT offer high signal-to-noise ratio and is ideal for studying fast / single molecular adhesion processes, quantitative interpretation of experimental results has been challenging. Here we used computational approach to investigate how TGT fluorescence readout can be quantitatively interpreted. In particular we studied force sensors made of a single TGT, multiplexed single TGTs, and two TGTs connected in series. Our results showed that fluorescence readout using a single TGT can result from drastically different combinations of force history and adhesion event density that span orders of magnitude. In addition, the apparent behaviour of the TGT is influenced by the tethered receptor-ligand, making it necessary to calibrate the TGT with every new receptor-ligand. To solve this problem, we proposed a system of two serially connected TGTs. Our result shows that not only is the ratiometric readout of serial TGT independent of the choice of receptor-ligand, it is able to reconstruct force history with sub-pN force resolution. This is also not possible by simply multiplexing different types of TGTs together. Lastly, we systematically investigated how sequence composition of the two serially connected TGTs can be tuned to achieve different dynamic range. This computational study demonstrated how serially connected irreversible molecular dissociation processes can accurately quantify molecular force, and laid the foundation for subsequent experimental studies.

Tuning cell adhesion on supported lipid bilayers via nanoscale geometry

Soft Matter ◽  
2021 ◽  
Author(s):  
Long Li ◽  
Jie Gao ◽  
Yingfeng Shao ◽  
Fan Song ◽  
Jinglei Hu
Keyword(s):  
Cell Adhesion ◽  
Lipid Bilayers ◽  
Model Systems ◽  
Supported Lipid Bilayers ◽  
Membrane Interface ◽  
Receptor Ligand ◽  
Ligand Interactions

The adhesion of cells to supported lipid bilayers (SLBs) has been widely used as model systems to study the receptor-ligand interactions that cause the membrane interface. The ligand-functionalized SLBs are...

Matrix mechanics and receptor–ligand interactions in cell adhesion

2010 ◽  
Vol 8 (2) ◽  
pp. 299-304 ◽  
Author(s):  
Dewi Harjanto ◽  
Muhammad H. Zaman
Keyword(s):  
Cell Adhesion ◽  
Receptor Ligand ◽  
Matrix Mechanics ◽  
Ligand Interactions

Probe stiffness regulates receptor-ligand bond lifetime under force

2011 ◽  
Vol 54 (5) ◽  
pp. 923-929 ◽  
Author(s):  
Yan Zhang ◽  
ShouQin Lü ◽  
Mian Long
Keyword(s):  
Receptor Ligand ◽  
Ligand Bond

scholarly journals Memory in receptor-ligand-mediated cell adhesion

2007 ◽  
Vol 104 (46) ◽  
pp. 18037-18042 ◽  
Author(s):  
V. I. Zarnitsyna ◽  
J. Huang ◽  
F. Zhang ◽  
Y.-H. Chien ◽  
D. Leckband ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Receptor Ligand

Modeling of cell adhesion and deformation mediated by receptor–ligand interactions

2015 ◽  
Vol 15 (2) ◽  
pp. 371-387 ◽  
Author(s):  
Amirreza F. Golestaneh ◽  
Ben Nadler
Keyword(s):  
Cell Adhesion ◽  
Receptor Ligand ◽  
Ligand Interactions
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