Asymmetric Molecular Friction in Supported Phospholipid Bilayers Revealed by NMR Measurements of Lipid Diffusion

Planar-supported phospholipid bilayers are responsive surfaces that reconstruct when macromolecules adsorb. This review outlines the phenomenon of lipid diffusion “slaved” to or significantly controlled by that of macromolecular adsorbates. To elucidate such systems, we discuss the value of spatially resolved experiments at the few-molecule level, lipid diffusion compared in outer and inner leaflets of the supported bilayer, and a simple method to minimize defects by the strategy of “electrostatic stitching.”

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Transbilayer coupling of obstructed lipid diffusion in polymer-tethered phospholipid bilayers

Soft Matter ◽

10.1039/b800801a ◽

2008 ◽

Vol 4 (9) ◽

pp. 1899 ◽

Cited By ~ 36

Author(s):

Miranda A. Deverall ◽

Sumit Garg ◽

Karin Lüdtke ◽

Rainer Jordan ◽

Jürgen Rühe ◽

...

Keyword(s):

Phospholipid Bilayers ◽

Lipid Diffusion

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First Nano-Infrared Spectroscopy and Imaging Measurements of Single Phospholipid Bilayers

10.26434/chemrxiv.5459617.v2 ◽

2018 ◽

Author(s):

Adrian Cernescu ◽

Michał Szuwarzyński ◽

Urszula Kwolek ◽

Karol Wolski ◽

Paweł Wydro ◽

...

Keyword(s):

Infrared Spectroscopy ◽

Ir Spectroscopy ◽

Absorption Band ◽

Spectral Region ◽

Near Field ◽

Phospholipid Bilayers ◽

Model Systems ◽

Absorption Bands ◽

Protein Complement ◽

20 Nm

<div><div>Scattering-mode Scanning Near-Field Optical Microscopy (sSNOM) allows one to obtain absorption spectra in the mid-IR region for samples as small as 20 nm in size. This configuration has made it possible to measure FTIR spectra of the protein complement of membranes. (Amenabar 2013) We now show that mid-IR sSNOM has the sensitivity required to measure spectra of phospholipids in individual bilayers in the spectral range 800 cm<sup>-1</sup>–1400 cm<sup>-1</sup>. We have observed the main absorption bands of the dipalmitoylphosphatidylcholine headgroups in this spectral region above noise level. We have also mapped the phosphate absorption band at 1070 cm<sup>-1</sup> simultaneously with the AFM topography. We have shown that we could achieve sufficient contrast to discriminate between single and multiple phospholipid bilayers and other structures, such as liposomes. This work opens the way to further research that uses nano-IR spectroscopy to describe the biochemistry of cell membranes and model systems.</div></div><div></div>

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Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

10.26434/chemrxiv.7505330 ◽

2018 ◽

Author(s):

Luke Jordan ◽

Nathan Wittenberg

Keyword(s):

Lipid Bilayers ◽

Binding Kinetics ◽

Supported Lipid Bilayers ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Supported Lipid Bilayer ◽

Head Groups ◽

Lipid Diffusion ◽

Kinetics Of ◽

Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

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