Light scattering from poly(L-glutamic acid) in aqueous solution in the helix-coil transition region

1984 ◽  
Vol 17 (4) ◽  
pp. 659-663 ◽  
Author(s):  
Akinori Kidera ◽  
Akio Nakajima
Keyword(s):  
Aqueous Solution ◽  
Light Scattering ◽  
Glutamic Acid ◽  
Transition Region ◽  
Coil Transition

Carbon-13 Chemical Shifts Accompanying Helix Formation

1973 ◽  
Vol 51 (4) ◽  
pp. 460-464 ◽  
Author(s):  
James R. Lyerla Jr. ◽  
Brian H. Barber ◽  
Murray H. Freedman
Keyword(s):  
Aqueous Solution ◽  
Nuclear Magnetic Resonance ◽  
Glutamic Acid ◽  
Chemical Shifts ◽  
Biological Significance ◽  
Structure Change ◽  
Shielding Effect ◽  
Amino Terminal ◽  
Helix Formation ◽  
Coil Transition

The helix–coil transition in an aqueous solution of poly-L-glutamic acid has been observed using 13C nuclear magnetic resonance. The transition from coil to helix results in pronounced deshielding of the carbonyl and C-α carbons of the polymer and a less dramatic shielding effect on the C-β carbon. These results are contrasted with the titration behavior of L-glutamic acid to delineate those shifts directly attributed to the secondary-structure change. The biological significance of this observation is supported by the 13C chemical shift differences observed for the 13C-enriched phenylalanyl residue 8 in the free 1–15 amino-terminal peptide of ribonuclease (RNase) and the 1–15 peptide:RNase S-protein complex.

HELIX-COIL TRANSITION OF POLY-α,L-GLUTAMIC ACID IN AQUEOUS SOLUTION STUDIED BY THE DISSOCIATION FIELD EFFECT RELAXATION METHOD

1973 ◽  
Vol 2 (4) ◽  
pp. 405-408 ◽  
Author(s):  
Tatsuya Yasunaga ◽  
Takayuki Sano ◽  
Kojiro Takahashi ◽  
Hidetoshi Takenaka ◽  
Satoshi Ito
Keyword(s):  
Aqueous Solution ◽  
Glutamic Acid ◽  
Field Effect ◽  
Relaxation Method ◽  
Coil Transition
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