Validation of In Vitro Cell-Based Human Blood−Brain Barrier Model Using Clinical Positron Emission Tomography Radioligands To Predict In Vivo Human Brain Penetration

2010 ◽  
Vol 7 (5) ◽  
pp. 1805-1815 ◽  
Author(s):  
Aloïse Mabondzo ◽  
Michel Bottlaender ◽  
Anne-Cécile Guyot ◽  
Katya Tsaouin ◽  
Jean Robert Deverre ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Human Brain ◽  
Human Blood ◽  
Emission Tomography ◽  
Brain Penetration ◽  
Positron Emission ◽  
Blood Brain Barrier Model ◽  
Barrier Model

scholarly journals Development of a blood sample detector for multi-tracer positron emission tomography using gamma spectroscopy

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Carlos Velasco ◽  
Adriana Mota-Cobián ◽  
Jesús Mateo ◽  
Samuel España
Keyword(s):  
Positron Emission Tomography ◽  
Blood Sample ◽  
Pet Imaging ◽  
Spectroscopic Analysis ◽  
Gamma Spectroscopy ◽  
Emission Tomography ◽  
Blood Samples ◽  
Positron Emission

Abstract Background Multi-tracer positron emission tomography (PET) imaging can be accomplished by applying multi-tracer compartment modeling. Recently, a method has been proposed in which the arterial input functions (AIFs) of the multi-tracer PET scan are explicitly derived. For that purpose, a gamma spectroscopic analysis is performed on blood samples manually withdrawn from the patient when at least one of the co-injected tracers is based on a non-pure positron emitter. Alternatively, these blood samples required for the spectroscopic analysis may be obtained and analyzed on site by an automated detection device, thus minimizing analysis time and radiation exposure of the operating personnel. In this work, a new automated blood sample detector based on silicon photomultipliers (SiPMs) for single- and multi-tracer PET imaging is presented, characterized, and tested in vitro and in vivo. Results The detector presented in this work stores and analyzes on-the-fly single and coincidence detected events. A sensitivity of 22.6 cps/(kBq/mL) and 1.7 cps/(kBq/mL) was obtained for single and coincidence events respectively. An energy resolution of 35% full-width-half-maximum (FWHM) at 511 keV and a minimum detectable activity of 0.30 ± 0.08 kBq/mL in single mode were obtained. The in vivo AIFs obtained with the detector show an excellent Pearson’s correlation (r = 0.996, p < 0.0001) with the ones obtained from well counter analysis of discrete blood samples. Moreover, in vitro experiments demonstrate the capability of the detector to apply the gamma spectroscopic analysis on a mixture of 68Ga and 18F and separate the individual signal emitted from each one. Conclusions Characterization and in vivo evaluation under realistic experimental conditions showed that the detector proposed in this work offers excellent sensibility and stability. The device also showed to successfully separate individual signals emitted from a mixture of radioisotopes. Therefore, the blood sample detector presented in this study allows fully automatic AIFs measurements during single- and multi-tracer PET studies.

scholarly journals In Vitro and In Vivo Evaluation of Bifunctional Bisthiosemicarbazone64Cu-Complexes for the Positron Emission Tomography Imaging of Hypoxia

2008 ◽  
Vol 51 (10) ◽  
pp. 2985-2991 ◽  
Author(s):  
Paul D. Bonnitcha ◽  
Amy L. Va̅vere ◽  
Jason S. Lewis ◽  
Jonathan R. Dilworth
Keyword(s):  
Positron Emission Tomography ◽  
Positron Emission Tomography Imaging ◽  
Emission Tomography ◽  
In Vivo Evaluation ◽  
Tomography Imaging ◽  
Positron Emission

Demonstration of high monoaminoxidase-A levels in neuroendocrine gastroenteropancreatic tumors in vitro and in vivo–tumor visualization using positron emission tomography with 11C-harmine

2003 ◽  
Vol 30 (6) ◽  
pp. 669-679 ◽  
Author(s):  
H. Örlefors ◽  
A. Sundin ◽  
K-J. Fasth ◽  
K. Öberg ◽  
B. Långström ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Emission Tomography ◽  
Tumor Visualization ◽  
Positron Emission

Synthesis, radiolabeling, in vitro and in vivo evaluation of [18F]-FPECMO as a positron emission tomography radioligand for imaging the metabotropic glutamate receptor subtype 5

2009 ◽  
Vol 36 (6) ◽  
pp. 613-622 ◽  
Author(s):  
Christophe Lucatelli ◽  
Michael Honer ◽  
Jean-Frédéric Salazar ◽  
Tobias L. Ross ◽  
P. August Schubiger ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Emission Tomography ◽  
Receptor Subtype ◽  
In Vivo Evaluation ◽  
Metabotropic Glutamate ◽  
Positron Emission

[76Br]bromodeoxyuridine, a potential tracer for the measurement of cell proliferation by positron emission tomography, in vitro and in vivo studies in mice

1999 ◽  
Vol 26 (6) ◽  
pp. 673-679 ◽  
Author(s):  
J.-E. Ryser ◽  
P. Bläuenstein ◽  
N. Rémy ◽  
R. Weinreich ◽  
P.H. Hasler ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Cell Proliferation ◽  
Emission Tomography ◽  
In Vivo Studies ◽  
Positron Emission
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