Cold Acclimation Proteome Analysis Reveals Close Link between the Up-Regulation of Low-Temperature Associated Proteins and Vernalization Fulfillment

2010 ◽  
Vol 9 (11) ◽  
pp. 5658-5667 ◽  
Author(s):  
Elham Sarhadi ◽  
Siroos Mahfoozi ◽  
Seyed Abdollah Hosseini ◽  
Ghasem Hosseini Salekdeh
Keyword(s):  
Low Temperature ◽  
Cold Acclimation ◽  
Proteome Analysis ◽  
Close Link ◽  
Associated Proteins

scholarly journals Dissecting the Roles of Cuticular Wax in Plant Resistance to Shoot Dehydration and Low-Temperature Stress in Arabidopsis

2021 ◽  
Vol 22 (4) ◽  
pp. 1554
Author(s):  
Tawhidur Rahman ◽  
Mingxuan Shao ◽  
Shankar Pahari ◽  
Prakash Venglat ◽  
Raju Soolanayakanahally ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Low Temperature ◽  
Cold Acclimation ◽  
Water Loss ◽  
Cuticular Wax ◽  
Dehydration Stress ◽  
Wax Deposition ◽  
Cuticular Waxes ◽  
Gas Chromatography Mass Spectroscopy

Cuticular waxes are a mixture of hydrophobic very-long-chain fatty acids and their derivatives accumulated in the plant cuticle. Most studies define the role of cuticular wax largely based on reducing nonstomatal water loss. The present study investigated the role of cuticular wax in reducing both low-temperature and dehydration stress in plants using Arabidopsis thaliana mutants and transgenic genotypes altered in the formation of cuticular wax. cer3-6, a known Arabidopsis wax-deficient mutant (with distinct reduction in aldehydes, n-alkanes, secondary n-alcohols, and ketones compared to wild type (WT)), was most sensitive to water loss, while dewax, a known wax overproducer (greater alkanes and ketones compared to WT), was more resistant to dehydration compared to WT. Furthermore, cold-acclimated cer3-6 froze at warmer temperatures, while cold-acclimated dewax displayed freezing exotherms at colder temperatures compared to WT. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis identified a characteristic decrease in the accumulation of certain waxes (e.g., alkanes, alcohols) in Arabidopsis cuticles under cold acclimation, which was additionally reduced in cer3-6. Conversely, the dewax mutant showed a greater ability to accumulate waxes under cold acclimation. Fourier Transform Infrared Spectroscopy (FTIR) also supported observations in cuticular wax deposition under cold acclimation. Our data indicate cuticular alkane waxes along with alcohols and fatty acids can facilitate avoidance of both ice formation and leaf water loss under dehydration stress and are promising genetic targets of interest.

Proteome analysis of actin filament-associated proteins in the postnatal rat cerebellum

Neuroscience ◽  
2012 ◽  
Vol 227 ◽  
pp. 90-101 ◽  
Author(s):  
N. Shi ◽  
C. Tian ◽  
X. Liang ◽  
P. Jiang ◽  
L. Liang ◽  
...  
Keyword(s):  
Actin Filament ◽  
Proteome Analysis ◽  
Rat Cerebellum ◽  
Associated Proteins

Low Temperature Induced Biochemical Mechanisms: Implications for Cold Acclimation and De-Acclimation

1993 ◽  
pp. 647-657 ◽  
Author(s):  
C. Stushnoff ◽  
R. L. Remmele ◽  
V. Essensee ◽  
M. McNeil
Keyword(s):  
Low Temperature ◽  
Cold Acclimation ◽  
Biochemical Mechanisms

Proteome Analysis of Cold Acclimation in Sunflower

2011 ◽  
Vol 10 (5) ◽  
pp. 2330-2346 ◽  
Author(s):  
Tiago S. Balbuena ◽  
Joaquín J. Salas ◽  
Enrique Martínez-Force ◽  
Rafael Garcés ◽  
Jay J. Thelen
Keyword(s):  
Cold Acclimation ◽  
Proteome Analysis

Extraction and Proteome Analysis of Starch Granule-Associated Proteins in Mature Wheat Kernel (Triticum aestivumL.)

2010 ◽  
Vol 9 (6) ◽  
pp. 3299-3310 ◽  
Author(s):  
Emmanuelle Bancel ◽  
Hélène Rogniaux ◽  
Clément Debiton ◽  
Christophe Chambon ◽  
Gérard Branlard
Keyword(s):  
Starch Granule ◽  
Proteome Analysis ◽  
Wheat Kernel ◽  
Associated Proteins

scholarly journals Independent Activation of Cold Acclimation by Low Temperature and Short Photoperiod in Hybrid Aspen

2002 ◽  
Vol 129 (4) ◽  
pp. 1633-1641 ◽  
Author(s):  
Annikki Welling ◽  
Thomas Moritz ◽  
E. Tapio Palva ◽  
Olavi Junttila
Keyword(s):  
Low Temperature ◽  
Cold Acclimation ◽  
Short Photoperiod ◽  
Hybrid Aspen

Cold Acclimation and Prolonged Low Temperature Survival of Filth Fly Parasites (Hymenoptera: Pteromalidae)

1986 ◽  
Vol 15 (4) ◽  
pp. 936-942 ◽  
Author(s):  
D. R. Guzman ◽  
J. J. Petersen
Keyword(s):  
Low Temperature ◽  
Cold Acclimation

scholarly journals Adaptation of Bacillus subtilis to growth at low temperature: a combined transcriptomic and proteomic appraisal

Microbiology ◽  
2006 ◽  
Vol 152 (3) ◽  
pp. 831-853 ◽  
Author(s):  
Ina Budde ◽  
Leif Steil ◽  
Christian Scharf ◽  
Uwe Völker ◽  
Erhard Bremer
Keyword(s):  
Bacillus Subtilis ◽  
Low Temperature ◽  
Response Regulator ◽  
Proteome Analysis ◽  
Atp Synthesis ◽  
Fine Tuning ◽  
Transcriptional Responses ◽  
Natural Habitats ◽  
Regulatory Circuit ◽  
Proteomic Approach

The soil bacterium Bacillus subtilis frequently encounters a reduction in temperature in its natural habitats. Here, a combined transcriptomic and proteomic approach has been used to analyse the adaptational responses of B. subtilis to low temperature. Propagation of B. subtilis in minimal medium at 15 °C triggered the induction of 279 genes and the repression of 301 genes in comparison to cells grown at 37 °C. The analysis thus revealed profound adjustments in the overall gene expression profile in chill-adapted cells. Important transcriptional changes in low-temperature-grown cells comprise the induction of the SigB-controlled general stress regulon, the induction of parts of the early sporulation regulons (SigF, SigE and SigG) and the induction of a regulatory circuit (RapA/PhrA and Opp) that is involved in the fine-tuning of the phosphorylation status of the Spo0A response regulator. The analysis of chill-stress-repressed genes revealed reductions in major catabolic (glycolysis, oxidative phosphorylation, ATP synthesis) and anabolic routes (biosynthesis of purines, pyrimidines, haem and fatty acids) that likely reflect the slower growth rates at low temperature. Low-temperature repression of part of the SigW regulon and of many genes with predicted functions in chemotaxis and motility was also noted. The proteome analysis of chill-adapted cells indicates a major contribution of post-transcriptional regulation phenomena in adaptation to low temperature. Comparative analysis of the previously reported transcriptional responses of cold-shocked B. subtilis cells with this data revealed that cold shock and growth in the cold constitute physiologically distinct phases of the adaptation of B. subtilis to low temperature.

Proteome analysis of microtubule-associated proteins and their interacting partners from mammalian brain

Amino Acids ◽  
2010 ◽  
Vol 41 (2) ◽  
pp. 363-385 ◽  
Author(s):  
Frank Kozielski ◽  
Tahira Riaz ◽  
Salvatore DeBonis ◽  
Christian J. Koehler ◽  
Mario Kroening ◽  
...  
Keyword(s):  
Proteome Analysis ◽  
Mammalian Brain ◽  
Microtubule Associated Proteins ◽  
Associated Proteins

scholarly journals Characterization of the Novel Mitochondrial Genome Segregation Factor TAP110 in Trypanosoma brucei

2021 ◽  
pp. jcs.254300
Author(s):  
Simona Amodeo ◽  
Ana Kalichava ◽  
Albert Fradera-Sola ◽  
Eloïse Bertiaux-Lequoy ◽  
Paul Guichard ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Trypanosoma Brucei ◽  
Proteome Analysis ◽  
Protozoan Parasite ◽  
Eukaryotic Cell ◽  
The Novel ◽  
Precise Position ◽  
Associated Proteins ◽  
First Time

Proper mitochondrial genome inheritance is important for eukaryotic cell survival. Trypanosoma brucei, a protozoan parasite, contains a singular mitochondrial genome, the kDNA. The kDNA is anchored to the basal body via the tripartite attachment complex (TAC) to ensure proper segregation. Several components of the TAC have been described. However, the connection of the TAC to the kDNA remains elusive. Here, we characterize the TAC associated protein TAP110. Depletion as well as overexpression of TAP110 leads to a delay in the separation of the replicated kDNA networks. Proteome analysis after TAP110 overexpression identified several kDNA associated proteins including a TEX-like protein that dually localizes to the nucleus and the kDNA potentially linking replication/segregation in the two compartments. The assembly of TAP110 into the TAC region seems to require the TAC but not the kDNA itself, however once TAP110 has been assembled it also interacts with the kDNA. Finally, for the first time we use ultrastructure expansion microscopy in trypanosomes to reveal the precise position of TAP110 between TAC102 and the kDNA, showcasing the potential of this approach.

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