The Equine Estrogen Metabolite 4-Hydroxyequilenin Causes DNA Single-Strand Breaks and Oxidation of DNA Bases in Vitro

1998 ◽  
Vol 11 (9) ◽  
pp. 1105-1111 ◽  
Author(s):  
Yumei Chen ◽  
Li Shen ◽  
Fagen Zhang ◽  
Serrine S. Lau ◽  
Richard B. van Breemen ◽  
...  
Keyword(s):  
Single Strand ◽  
Dna Bases ◽  
Strand Breaks ◽  
Estrogen Metabolite ◽  
Oxidation Of Dna ◽  
Single Strand Breaks

Chromosomal aberrations and deoxyribonucleic acid single-strand breaks in adipose-derived stem cells during long-term expansion in vitro

Cytotherapy ◽  
2013 ◽  
Vol 15 (7) ◽  
pp. 767-781 ◽  
Author(s):  
Katrin Froelich ◽  
Johannes Mickler ◽  
Gudrun Steusloff ◽  
Antje Technau ◽  
Mario Ramos Tirado ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chromosomal Aberrations ◽  
Deoxyribonucleic Acid ◽  
Single Strand ◽  
Adipose Derived Stem Cells ◽  
Strand Breaks ◽  
Single Strand Breaks

Uranyl Acetate Causes DNA Single Strand Breaks In Vitro in the Presence of Ascorbate (Vitamin C)

2003 ◽  
Vol 16 (4) ◽  
pp. 524-530 ◽  
Author(s):  
Monica Yazzie ◽  
Shania L. Gamble ◽  
Edgar R. Civitello ◽  
Diane M. Stearns
Keyword(s):  
Vitamin C ◽  
Uranyl Acetate ◽  
Single Strand ◽  
Strand Breaks ◽  
Single Strand Breaks

Toxicity, Clastogenicity and Genotoxicity of Theophylline and Pentoxifylline in Mammalian Cells Cultured In Vitro

1995 ◽  
Vol 23 (4) ◽  
pp. 504-512
Author(s):  
Darina Slameňová ◽  
Ivan Chalupa ◽  
Alena Gábelová ◽  
Eva Bozsakyová ◽  
Eva Horváthová ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Term Treatment ◽  
Single Strand ◽  
Toxic Effects ◽  
Short Term ◽  
Short Term Treatment ◽  
V79 Cells ◽  
Strand Breaks ◽  
Single Strand Breaks

— As part of a developmental study on theophylline and pentoxifylline, these drugs were tested for possible cytotoxic, mutagenic and clastogenic effects on V79 hamster cells and human lymphocytes cultured in vitro. After the short-term treatment of V79 cells with theophylline and pentoxifylline, the cells were relatively resistant to the toxic effects of these methylxanthines. Generally, only high concentrations of theophylline or pentoxifylline had toxic effects on exposed V79 cells. Short-term treatment of V79 cells with theophylline or pentoxifylline did not induce 6-thioguanine resistant mutations in either the presence or absence of S9 fractions. However, in the absence of an S9 fraction, elevated levels of single-strand breaks in DNA were induced. Both methylxanthines caused clastogenic effects in human lymphocytes and hamster V79 cells after long-term exposure. We suggest that theophylline and pentoxifylline are clastogenic but not genotoxic, and that the molecular mechanism for the induction of single-strand breaks in DNA and the induction of chromosomal aberrations in cells treated with theophylline and pentoxifylline is not the induction of lesions in the DNA but the inhibition of DNA chain elongation.

Sign in / Sign up

Export Citation Format

Share Document