Structural Instability of Argillaceous Material

2004 ◽  
Vol 61 (5/6) ◽  
pp. 157-159
Author(s):  
E. I. Evtushenko ◽  
E. I. Kravtsov ◽  
I. Yu. Kashcheeva ◽  
O. K. Sysa
BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Janire Urrutia ◽  
Alejandra Aguado ◽  
Carolina Gomis-Perez ◽  
Arantza Muguruza-Montero ◽  
Oscar R. Ballesteros ◽  
...  

Abstract Background The amino acid sequence of proteins generally carries all the necessary information for acquisition of native conformations, but the vectorial nature of translation can additionally determine the folding outcome. Such consideration is particularly relevant in human diseases associated to inherited mutations leading to structural instability, aggregation, and degradation. Mutations in the KCNQ2 gene associated with human epilepsy have been suggested to cause misfolding of the encoded Kv7.2 channel. Although the effect on folding of mutations in some domains has been studied, little is known of the way pathogenic variants located in the calcium responsive domain (CRD) affect folding. Here, we explore how a Kv7.2 mutation (W344R) located in helix A of the CRD and associated with hereditary epilepsy interferes with channel function. Results We report that the epilepsy W344R mutation within the IQ motif of CRD decreases channel function, but contrary to other mutations at this site, it does not impair the interaction with Calmodulin (CaM) in vitro, as monitored by multiple in vitro binding assays. We find negligible impact of the mutation on the structure of the complex by molecular dynamic computations. In silico studies revealed two orientations of the side chain, which are differentially populated by WT and W344R variants. Binding to CaM is impaired when the mutated protein is produced in cellulo but not in vitro, suggesting that this mutation impedes proper folding during translation within the cell by forcing the nascent chain to follow a folding route that leads to a non-native configuration, and thereby generating non-functional ion channels that fail to traffic to proper neuronal compartments. Conclusions Our data suggest that the key pathogenic mechanism of Kv7.2 W344R mutation involves the failure to adopt a configuration that can be recognized by CaM in vivo but not in vitro.


Nanophotonics ◽  
2020 ◽  
Vol 9 (15) ◽  
pp. 4601-4608 ◽  
Author(s):  
Pengyu Zhuang ◽  
Hanyu Fu ◽  
Ning Xu ◽  
Bo Li ◽  
Jun Xu ◽  
...  

AbstractInterfacial solar vapor generation has revived the solar-thermal-based desalination due to its high conversion efficiency of solar energy. However, most solar evaporators reported so far suffer from severe salt-clogging problems during solar desalination, leading to performance degradation and structural instability. Here, we demonstrate a free-standing salt-rejecting reduced graphene oxide (rGO) membrane serving as an efficient, stable, and antisalt-fouling solar evaporator. The evaporation rate of the membrane reaches up to 1.27 kg m−2 h−1 (solar–thermal conversion efficiency ∼79%) under one sun, out of 3.5 wt% brine. More strikingly, due to the tailored narrow interlayer spacing, the rGO membrane can effectively reject ions, preventing salt accumulation even for high salinity brine (∼8 wt% concentration). With enabled salt-antifouling capability, flexibility, as well as stability, our rGO membrane serves as a promising solar evaporator for high salinity brine treatment.


2021 ◽  
pp. 219256822110308
Author(s):  
Yogesh Kishorkant Pithwa ◽  
Vikrant Sinha Roy

Study Design: Prospective Observational Study. Objectives: To assess the feasibility of utilizing SINS score, originally suggested for neoplastic conditions, to assess structural instability in spinal tuberculosis. Methods: Patients with an established diagnosis of spinal tuberculosis were included in the study. Based on SINS scoring, patients classified as those with “indeterminate stability” were managed with or without surgery based on other parameters including neurological status, severity of pain, medical comorbidities, etc. Results: Eighty [39 males, 41 females] patients prospectively evaluated with mean age 46.74 ± 17.3 years. Classification done into stable [n = 7], indeterminate [n = 45] and unstable [n = 28] groups based on SINS scoring. All the patients in unstable group were treated with surgical stabilization whereas none in the stable group required surgical stabilization. In the indeterminate group, 26 patients underwent surgical stabilization, while 19 treated non-operatively. Major determinants predisposing to surgical intervention in “indeterminate group” were pain [14 of 26 patients] and neurological status [11 of 26 patients]. Mean follow-up 38.5 ± 22.61 months with minimum follow-up being 24 months. Preoperative VAS score for pain improved from median of 9/10 to 1/10 following surgery [ P < .0001]. In the non-operative group, the improvement was from median score of 6/10 to 1/10 [ P < .0001]. Preoperative ODI improved in non-operative and operative group from median of 42% and 70%, respectively to 10% and 12%, respectively in the postoperative period [ P < .0001 for both groups]. Conclusions: SINS scoring can be a helpful tool in surgical decision-making even in spinal tuberculosis. Further refinement of the score can be done with a larger, multicenter study.


Mathematics ◽  
2021 ◽  
Vol 9 (13) ◽  
pp. 1519
Author(s):  
Mikulas Huba ◽  
Pavol Bistak ◽  
Damir Vrancic ◽  
Katarina Zakova

The article reviews the results of a number of recent papers dealing with the revision of the simplest approaches to the control of first-order time-delayed systems. The concise introductory review is extended by an analysis of two discrete-time approaches to dead-time compensation control of stable, integrating, and unstable first-order dead-time processes including simple diagnostics of the model used and focusing on the possibility of simplified but reliable plant modelling. The first approach, based on the first historically known dead-time compensator (DTC) with possible dead-beat performance, is based on the reconstruction of the actual process variables and the compensation of input disturbances by an extended state observer (ESO). Such solutions play an important role both in a disturbance observer (DOB) based control and in an active disturbance rejection control (ADRC). The second approach considered comes from the Smith predictor with two degrees of freedom, which combines feedforward control with output disturbance reconstruction and compensation by the parallel plant model. It is shown that these two approaches offer advantageous properties in the case of actuator limitations, in contrast to the commonly used PID controllers. However, when applied to integrating and unstable first-order systems, the unconstrained and possibly unobservable output disturbance signal of the second solution must be eliminated from the control loop, due to the hidden structural instability of the Smith predictor-like solutions. The modified solutions, usually referred to as filtered Smith predictor (FSP), then no longer provide a disturbance signal and thus no longer fully fit into the concept of Industry 4.0, which is focused on further optimization, predictive maintenance in dynamic systems, diagnosis, fault detection and fault identification of dynamic processes and forms the basis for the digitalization of smart production. Nevertheless, the detailed analysis of the elimination of the unstable disturbance response mode is also worth mentioning in terms of other possible solutions. The application of both approaches to the control of a thermal process shows almost equivalent quality, but with different dependencies on the tuning parameters used. It is confirmed that a more detailed identification of the controlled process and the resulting higher complexity of the control algorithms does not necessarily lead to an increase in the resulting quality of the transients, which underlines the importance of the simplified plant modelling for practice.


2016 ◽  
Vol 2 ◽  
pp. 477-484 ◽  
Author(s):  
Yurii Meshcheryakov ◽  
Alexandre Divakov ◽  
Natali Zhigacheva ◽  
Grigorii Konovalov

2014 ◽  
Vol 938 ◽  
pp. 253-256
Author(s):  
Hashlina Rusdi ◽  
Norlida Kamarulzaman ◽  
Rusdi Roshidah ◽  
Kelimah Elong ◽  
Abd Rahman Azilah

Layered LiNi1-xCoxO2 is one of the promising cathode materials for Li-ion battery application. However, the Ni rich cathode materials exhibit low capacity and bad capacity retention. This is due to factors such as disorder and structural instability when Li is removed during charge-discharge. Overlithiation of cathode materials is expected to improve the cation ordering and structural stability. Good cation ordering will increase the battery capacity. During charge-discharge, the irreversible Li+ loss can be replaced to a certain extent by the interstitial Li+ ions in the lattice of the LixNi0.8Co0.2O2 material. This helps reduce capacity fading of the cathode materials. In this work the overlithiation of LiNi0.8Co0.2O2 is done by interstitially doping Li+ in the LiNi0.8Co0.2O2 materials producing Li1.05Ni0.8Co0.2O2 and Li1.1Ni0.8Co0.2O2. Results showthat the performance of the overlithiated LiNi0.8Co0.2O2 materials is better than pure LiNi0.8Co0.2O2.


2012 ◽  
Vol 86 (10) ◽  
Author(s):  
Daigorou Hirai ◽  
F. von Rohr ◽  
R. J. Cava

2012 ◽  
Vol 85 (11) ◽  
pp. 939-948 ◽  
Author(s):  
K. Roleder ◽  
A. Bussmann-Holder ◽  
M. Górny ◽  
K. Szot ◽  
A.M. Glazer

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