Induction of embryogenic mango cultures as affected by genotype, explanting, 2,4-D and embryogenic nurse culture

1998 ◽  
Vol 53 (1) ◽  
pp. 13-18 ◽  
Author(s):  
R.E. Litz ◽  
R.C. Hendrix ◽  
P.A. Moon ◽  
V. M. Chavez
Keyword(s):  
Nurse Culture

High frequency plant regeneration from rice protoplasts by novel nurse culture methods

1987 ◽  
Vol 206 (3) ◽  
pp. 408-413 ◽  
Author(s):  
Junko Kyozuka ◽  
Yasuyuki Hayashi ◽  
Ko Shimamoto
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Culture Methods ◽  
Nurse Culture

The use of nurse culture in the development of haploid clones in tomato

Planta ◽  
1972 ◽  
Vol 104 (4) ◽  
pp. 357-361 ◽  
Author(s):  
W. R. Sharp ◽  
R. S. Raskin ◽  
H. E. Sommer
Keyword(s):  
Nurse Culture

Efficient plant regeneration from protoplasts of Arachis paraguariensis Chod. et Hassl. using a nurse culture method

1993 ◽  
Vol 34 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Zhijian Li ◽  
Robert L. Jarret ◽  
Roy N. Pittman ◽  
Kerry B. Dunbar ◽  
James W. Demski
Keyword(s):  
Plant Regeneration ◽  
Culture Method ◽  
Nurse Culture ◽  
Arachis Paraguariensis

scholarly journals Explant-nurse Culture of Protoplasts and Plant Regeneration in the Radish.

1996 ◽  
Vol 13 (2) ◽  
pp. 127-132
Author(s):  
Kui Nan CAI ◽  
Toshinori ABE ◽  
Takeo SASAHARA
Keyword(s):  
Plant Regeneration ◽  
Nurse Culture

Induction of callus formation from difficile date palm protoplasts by means of nurse culture

2007 ◽  
Vol 330 (5) ◽  
pp. 392-401 ◽  
Author(s):  
Djamila Chabane ◽  
Akym Assani ◽  
Nadia Bouguedoura ◽  
Robert Haïcour ◽  
Georges Ducreux
Keyword(s):  
Date Palm ◽  
Callus Formation ◽  
Nurse Culture

Comparative studies of protoplast development in jack pine (Pinus banksiana)

1990 ◽  
Vol 68 (8) ◽  
pp. 1774-1779 ◽  
Author(s):  
T. E. Tautorus ◽  
L. C. Fowke ◽  
D. I. Dunstan
Keyword(s):  
Cell Suspension ◽  
Comparative Studies ◽  
Pinus Banksiana ◽  
Culture Medium ◽  
Solid Medium ◽  
Jack Pine ◽  
Culture Conditions ◽  
Nurse Culture ◽  
Protoplasts Regeneration ◽  
Small Clusters

Protoplasts were isolated either from the cotyledons of 8- to 10-day-old seedlings, or from cell suspensions, initiated from seedlings of the same open-pollinated seedlot of jack pine (Pinus banksiana Lamb.). Protoplast yields were 2.0 – 7.0 × 105/g fresh wt. for cotyledon-derived, and 0.3–5 × 105/g fresh wt. for suspension-derived protoplasts. Cotyledon protoplasts (6.8%) divided after 5 to 8 days incubation in liquid Lainé medium C with 450 mM glucose as osmoticum. Small clusters of 6 to 10 cells, which were observed after approximately 2 weeks, formed microcalli upon further dilution of the culture medium. By 6 to 8 weeks, these microcalli could be transferred to solid medium using a nurse culture of jack pine cells. Protoplasts isolated from seven cell suspension lines differed in their responses to culture conditions. First divisions were only observed with two cell lines cultured in either Lainé medium C or one-half Litvay with 370 mM glucose, but this did not lead to callus development. Key words: jack pine, Pinus banksiana, cotyledons, protoplasts, regeneration, callus.

Sign in / Sign up

Export Citation Format

Share Document