Effect of nurse culture and conditioned medium on colony formation and plant regeneration from Lolium perenne protoplasts

Plant Science ◽  
1995 ◽  
Vol 108 (2) ◽  
pp. 229-239 ◽  
Author(s):  
Marianne Folling ◽  
Sten Madsen ◽  
Annette Olesen
Keyword(s):  
Plant Regeneration ◽  
Conditioned Medium ◽  
Lolium Perenne ◽  
Colony Formation ◽  
Nurse Culture

scholarly journals Inhibition against CFU-C and CFU-E colony formation by soluble factor(s) derived from hairy cells

Blood ◽  
1989 ◽  
Vol 73 (4) ◽  
pp. 907-913 ◽  
Author(s):  
N Taniguchi ◽  
H Kuratsune ◽  
A Kanamaru ◽  
Y Tokumine ◽  
S Tagawa ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Colony Formation ◽  
Inhibitory Effect ◽  
Lymphocytic Leukemia ◽  
Hairy Cell ◽  
Cell Leukemia ◽  
Hairy Cells ◽  
Cell Conditioned Medium ◽  
Normal Controls

Abstract The inhibitory effect by hairy cell conditioned medium (HCCM) on the growth of granulocyte and erythrocyte colony forming cells was studied in vitro. The percent inhibition of CFU-C formation by HCCM from four hairy cell leukemia (HCL) patients ranged from 36% to 76%, while no inhibition was observed with conditioned medium (CM) obtained from three B-cell chronic lymphocytic leukemia (B-CLL) patients nor from two normal controls. HCCM inhibited specially the growth of rG-CSF responding stem cells. The hairy cell-derived colony inhibitory factor from HCCM was nondialyzable, fairly stable to heat treatment, and trypsin sensitive. Its maximal inhibitory activity against granulopoiesis was observed in the fractions of 5,000 to 6,000 daltons. Moreover HCCM inhibited CFU-E colony formation but not BFU-E. These results indicate that hairy cells produce a factor that inhibits granulopoiesis and erythropoiesis in vitro. This factor may play a role in neutropenia and anemia observed in HCL.

Plant regeneration from suspension cultures and protoplasts of Lolium perenne L

Plant Science ◽  
1989 ◽  
Vol 63 (2) ◽  
pp. 167-176 ◽  
Author(s):  
J. Creemers-Molenaar ◽  
P. Van Der Valk ◽  
J.P.M. Loeffen ◽  
M.A.C.M. Zaal
Keyword(s):  
Plant Regeneration ◽  
Lolium Perenne ◽  
Suspension Cultures ◽  
Lolium Perenne L

High frequency plant regeneration from rice protoplasts by novel nurse culture methods

1987 ◽  
Vol 206 (3) ◽  
pp. 408-413 ◽  
Author(s):  
Junko Kyozuka ◽  
Yasuyuki Hayashi ◽  
Ko Shimamoto
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Culture Methods ◽  
Nurse Culture

scholarly journals Selective stimulation by mouse spleen cell conditioned medium of human eosinophil colony formation

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 999-1005
Author(s):  
D Metcalf ◽  
RL Cutler ◽  
NA Nicola
Keyword(s):  
Spleen Cell ◽  
Conditioned Medium ◽  
Colony Formation ◽  
Culture System ◽  
Mouse Spleen ◽  
Mouse Spleen Cell ◽  
Human Eosinophil ◽  
Eosinophil Colonies ◽  
Cell Conditioned Medium ◽  
Marrow Cells

Stimulation of unfractionated or nonadherent human marrow cells in agar culture by pokeweed-mitogen-stimulated BALB/c mouse spleen cell conditioned medium (SCM) led, in most cultures, to the exclusive formation of eosinophil colonies. The culture system exhibited linearity of eosinophil colony formation with varying numbers of cells cultured, and the absolute numbers and size of SCM-stimulated eosinophil colonies approximated those in cultures stimulated by human placental conditioned medium. The active factor in SCM for human eosinophil colony formation was not clearly separable from the factors stimulating granulocyte-macrophage and eosinophil colony formation by mouse marrow cells on ammonium sulfate and phenyl boronate chromatography, but was of larger size than the mouse-active factors and separable from them by phenyl sepharose chromatography. This selective culture system for eosinophil colony formation should be of value for studies on human eosinophil progenitor and maturing cell populations in a variety of disease states.

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