Major basic protein, but not eosinophil cationic protein or eosinophil protein X, is related to atopy in cystic fibrosis

Abstract We evaluated the levels of mRNAs encoding cationic proteins in peripheral blood eosinophils (PBE) purified from patients with eosinophilia and in eosinophils differentiated from cord blood cells (CBC) by culture with recombinant human interleukin-3 (rhIL-3), rhGM- CSF, and rhIL-5. Messenger RNAs encoding eosinophil peroxidase (EPO), major basic protein (MBP), eosinophil-derived neurotoxin (EDN), and eosinophil cationic protein (ECP) were detected by Northern blot hybridization with the respective specific oligonucleotide probes. In mature PBE, MBP mRNA appeared to be absent, whereas EPO mRNA was barely detectable in only 5 of the 19 patients. In contrast, EDN and ECP mRNAs were observed in the PBE of all patients. In CE, EPO, and MBP, mRNAs were abundant in immature eosinophils and their amounts decreased after differentiation toward eosinophils. ECP and EDN mRNAs followed the same patterns, but mRNAs were less abundant at all timepoints studied. Study of mRNA t1/2 during the time course of differentiation indicated that changes in the stability of the different mRNAs were not responsible for the variations observed in the steady-state levels. Together, these results suggest that regulation of expression differs among EPO, MBP, EDN, and ECP mRNAs during the time course of eosinophil differentiation.

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Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique.

Journal of Histochemistry & Cytochemistry ◽

10.1177/34.11.3772075 ◽

1986 ◽

Vol 34 (11) ◽

pp. 1399-1403 ◽

Cited By ~ 48

Author(s):

A Egesten ◽

J Alumets ◽

C von Mecklenburg ◽

M Palmegren ◽

I Olsson

Keyword(s):

Basic Protein ◽

Intracellular Localization ◽

Protein A ◽

Eosinophil Cationic Protein ◽

Specific Marker ◽

Microscopic Technique ◽

Major Basic Protein ◽

Cationic Protein ◽

Eosinophil Peroxidase ◽

The Matrix

An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.

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Decreased expression of eosinophil peroxidase and major basic protein messenger RNAs during eosinophil maturation

Blood ◽

10.1182/blood.v79.10.2592.bloodjournal79102592 ◽

1992 ◽

Vol 79 (10) ◽

pp. 2592-2597 ◽

Cited By ~ 38

Author(s):

V Gruart ◽

MJ Truong ◽

J Plumas ◽

M Zandecki ◽

JP Kusnierz ◽

...

Keyword(s):

Time Course ◽

Basic Protein ◽

Blot Hybridization ◽

Eosinophil Cationic Protein ◽

Northern Blot Hybridization ◽

Messenger Rnas ◽

Major Basic Protein ◽

Regulation Of Expression ◽

Cationic Protein ◽

Eosinophil Peroxidase

We evaluated the levels of mRNAs encoding cationic proteins in peripheral blood eosinophils (PBE) purified from patients with eosinophilia and in eosinophils differentiated from cord blood cells (CBC) by culture with recombinant human interleukin-3 (rhIL-3), rhGM- CSF, and rhIL-5. Messenger RNAs encoding eosinophil peroxidase (EPO), major basic protein (MBP), eosinophil-derived neurotoxin (EDN), and eosinophil cationic protein (ECP) were detected by Northern blot hybridization with the respective specific oligonucleotide probes. In mature PBE, MBP mRNA appeared to be absent, whereas EPO mRNA was barely detectable in only 5 of the 19 patients. In contrast, EDN and ECP mRNAs were observed in the PBE of all patients. In CE, EPO, and MBP, mRNAs were abundant in immature eosinophils and their amounts decreased after differentiation toward eosinophils. ECP and EDN mRNAs followed the same patterns, but mRNAs were less abundant at all timepoints studied. Study of mRNA t1/2 during the time course of differentiation indicated that changes in the stability of the different mRNAs were not responsible for the variations observed in the steady-state levels. Together, these results suggest that regulation of expression differs among EPO, MBP, EDN, and ECP mRNAs during the time course of eosinophil differentiation.

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