Optimization of the fluorescence in situ hybridization (FISH) technique for high detection efficiency of very small proportions of target interphase nuclei

2001 ◽  
Vol 58 (4) ◽  
pp. 309-318 ◽  
Author(s):  
J Yan ◽  
E Guilbault ◽  
J Massé ◽  
M Bronsard ◽  
P DeGrandpré ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Detection Efficiency ◽  
Interphase Nuclei ◽  
Fish Technique ◽  
High Detection ◽  
High Detection Efficiency

scholarly journals Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2106
Author(s):  
Barbara Kij-Mitka ◽  
Halina Cernohorska ◽  
Svatava Kubickova ◽  
Sylwia Prochowska ◽  
Wojciech Niżański ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosomes ◽  
Chromosomal Abnormalities ◽  
Molecular Cytogenetics ◽  
Molecular Probes ◽  
Domestic Cat ◽  
Fish Technique ◽  
Y Chromosomes

Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.

Optimal preparation of preimplantation embryo interphase nuclei for analysis by fluorescence in-situ hybridization

1994 ◽  
Vol 9 (3) ◽  
pp. 533-537 ◽  
Author(s):  
Edith Coonen ◽  
John C. M. Dumoulin ◽  
Frans C. S. Ramaekers ◽  
Anton H. N. Hopman
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Preimplantation Embryo ◽  
Interphase Nuclei

scholarly journals Suspension (S)-FISH, a New Technique for Interphase Nuclei

2002 ◽  
Vol 50 (12) ◽  
pp. 1697-1698 ◽  
Author(s):  
Ulf Steinhaeuser ◽  
Heike Starke ◽  
Angela Nietzel ◽  
Joerg Lindenau ◽  
Peter Ullmann ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Interphase Nucleus ◽  
New Technique ◽  
Interphase Nuclei ◽  
First Results ◽  
A New Technique

We describe a versatile method for performing fluorescence in situ hybridization (FISH) in suspension instead of on a slide as usually done. This so-called suspension-FISH (S-FISH) opens new possibilities for the analysis of shape and functions of the human interphase nucleus. The procedure is described and the first results using this approach are presented.

scholarly journals Application of Fluorescence In Situ Hybridization (FISH) Technique for the Detection of Genetic Aberration in Medical Science

Cureus ◽  
2017 ◽  
Author(s):  
Zubair Ahmed Ratan ◽  
Sojib Bin Zaman ◽  
Varshil Mehta ◽  
Mohammad Faisal Haidere ◽  
Nusrat Jahan Runa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Medical Science ◽  
Fish Technique ◽  
Genetic Aberration

scholarly journals Procedure for whole mount fluorescence in situ hybridization of interphase nuclei on Arabidopsis thaliana

1994 ◽  
Vol 6 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Serge Bauwens ◽  
Katerina Katsanis ◽  
Marc Van Montagu ◽  
Patrick Van Oostveldt ◽  
Gilbert Engler
Keyword(s):  
Arabidopsis Thaliana ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Interphase Nuclei ◽  
Whole Mount
Sign in / Sign up

Export Citation Format

Share Document