scholarly journals Suspension (S)-FISH, a New Technique for Interphase Nuclei

2002 ◽  
Vol 50 (12) ◽  
pp. 1697-1698 ◽  
Author(s):  
Ulf Steinhaeuser ◽  
Heike Starke ◽  
Angela Nietzel ◽  
Joerg Lindenau ◽  
Peter Ullmann ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Interphase Nucleus ◽  
New Technique ◽  
Interphase Nuclei ◽  
First Results ◽  
A New Technique

We describe a versatile method for performing fluorescence in situ hybridization (FISH) in suspension instead of on a slide as usually done. This so-called suspension-FISH (S-FISH) opens new possibilities for the analysis of shape and functions of the human interphase nucleus. The procedure is described and the first results using this approach are presented.

Fluorescence in situ hybridization of pap smears in management of cervical cancer: A new technique

1995 ◽  
Vol 84 (2) ◽  
pp. 153 ◽  
Author(s):  
Kristine L. Kingsley ◽  
Aurelia M. Meloni ◽  
Lisa A. U'Ren ◽  
Michael T. Medchill ◽  
Angelica M. Zaid ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
New Technique ◽  
Pap Smears ◽  
A New Technique

scholarly journals Procedure for whole mount fluorescence in situ hybridization of interphase nuclei on Arabidopsis thaliana

1994 ◽  
Vol 6 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Serge Bauwens ◽  
Katerina Katsanis ◽  
Marc Van Montagu ◽  
Patrick Van Oostveldt ◽  
Gilbert Engler
Keyword(s):  
Arabidopsis Thaliana ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Interphase Nuclei ◽  
Whole Mount

Use of fluorescence in situ hybridization to study the arrangement of DNA sequences in normal and disease-related chromosomes

1995 ◽  
Vol 53 ◽  
pp. 748-749
Author(s):  
Barbara J. F. Trask ◽  
Hillary Massa ◽  
Cynthia Friedman ◽  
Richard Esposito ◽  
Ger van den Engh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequences ◽  
Single Copy ◽  
Two Dimensions ◽  
Genetic Maps ◽  
Epifluorescence Microscopy ◽  
Metaphase Chromosomes ◽  
Interphase Nuclei

The sites of specific DNA sequences can be fluorescently tagged by fluorescence in situ hybridization (FISH). Different sequences can be labeled with different fluorochromes so that their arrangement can be studied using epifluorescence microscopy. The distances between points on the same or different chromosomes can be determined easily in a large number of interphase nuclei or metaphase chromosomes. A variety of probe types, ranging from single-copy sequences to highly repeated sequences can be employed. Our work has focussed on the analysis of hybridization patterns in two dimensions using conventional fluorescence microscopy.We have used FISH to study various aspects of genome organization that are difficult to study using other techniques. Examples of these applications will be presented.FISH is now the method of choice for determining the chromosomal location of DNA sequences. DNA sequences can be positioned in the genome with <1:1000 accuracy (to a 3-Mbp region within a 3000-Mbp genome). Through FISH, the cytogenetic, physical and genetic maps of chromosomes can be linked.

Analysis of the retinoblastoma gene in interphase nuclei by fluorescence in situ hybridization

1991 ◽  
Vol 56 (1) ◽  
pp. 98
Author(s):  
A. Kallioniemi ◽  
O-P. Kallioniemi ◽  
L-C. Yu ◽  
D. Pinkel ◽  
F. Waldman ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Retinoblastoma Gene ◽  
Interphase Nuclei
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