Fluorescence In Situ Hybridization (FISH) Technique for the Micronucleus Test

2013 ◽  
pp. 237-244 ◽  
Author(s):  
Ilse Decordier ◽  
Micheline Kirsch-Volders
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Micronucleus Test ◽  
Fish Technique

scholarly journals Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2106
Author(s):  
Barbara Kij-Mitka ◽  
Halina Cernohorska ◽  
Svatava Kubickova ◽  
Sylwia Prochowska ◽  
Wojciech Niżański ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosomes ◽  
Chromosomal Abnormalities ◽  
Molecular Cytogenetics ◽  
Molecular Probes ◽  
Domestic Cat ◽  
Fish Technique ◽  
Y Chromosomes

Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.

Cytogenetic toxicity of cycloplatam in human lymphocytes: detection by the micronucleus test and fluorescence in situ hybridization

2006 ◽  
Vol 17 (3) ◽  
pp. 289-295 ◽  
Author(s):  
Armen Nersesyan ◽  
Emanuela Perrone ◽  
Paola Roggieri ◽  
Claudia Bolognesi
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Micronucleus Test ◽  
Human Lymphocytes

scholarly journals Application of Fluorescence In Situ Hybridization (FISH) Technique for the Detection of Genetic Aberration in Medical Science

Cureus ◽  
2017 ◽  
Author(s):  
Zubair Ahmed Ratan ◽  
Sojib Bin Zaman ◽  
Varshil Mehta ◽  
Mohammad Faisal Haidere ◽  
Nusrat Jahan Runa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Medical Science ◽  
Fish Technique ◽  
Genetic Aberration

Her2/neu gene amplification in breast carcinoma patients: Our experience with fluorescence in situ hybridization (FISH) technique

2012 ◽  
Vol 9 (2) ◽  
pp. 146-150
Author(s):  
R.N. Makroo ◽  
M. Chowdhry ◽  
A. Fauzdar ◽  
M. Mishra ◽  
P. Srivastava ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Breast Carcinoma ◽  
Fluorescence In Situ Hybridization ◽  
Gene Amplification ◽  
Fish Technique
Sign in / Sign up

Export Citation Format

Share Document