Oral pseudomembranous candidiasis, herpes simplex virus-1 infection, and oral mucositis in head and neck cancer patients receiving radiotherapy and granulocyte-macrophage colony-stimulating factor (GM-CSF) mouthwash

2001 ◽  
Vol 30 (8) ◽  
pp. 471-480 ◽  
Author(s):  
Ourania Nicolatou-Galitis ◽  
Konstantinos Dardoufas ◽  
Panagiotis Markoulatos ◽  
Anastasia Sotiropoulou-Lontou ◽  
Konstantinos Kyprianou ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Colony Stimulating Factor ◽  
Herpes Simplex Virus 1 ◽  
Gm Csf ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Simplex Virus ◽  
Stimulating Factor

scholarly journals The Development of Murine Plasmacytoid Dendritic Cell Precursors Is Differentially Regulated by FLT3-ligand and Granulocyte/Macrophage Colony-Stimulating Factor

2002 ◽  
Vol 195 (7) ◽  
pp. 953-958 ◽  
Author(s):  
Michel Gilliet ◽  
Andre Boonstra ◽  
Carine Paturel ◽  
Svetlana Antonenko ◽  
Xiu-Ling Xu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Herpes Simplex ◽  
Cpg Odn ◽  
Colony Stimulating Factor ◽  
Flt3 Ligand ◽  
Large Numbers ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Simplex Virus ◽  
Stimulating Factor

Plasmacytoid predendritic cells or type 1 interferon (IFN)-producing cells (IPCs) have recently been identified in mice. Although culture systems giving rise to different murine dendritic cell subsets have been established, the developmental regulation of murine plasmacytoid IPCs and the culture conditions leading to their generation remain unknown. Here we show that large numbers of over 40% pure CD11c+CD11b−B220+Gr-1+ IPCs can be generated from mouse bone marrow cultures with FLT3-ligand. By contrast GM-CSF or TNF-α, which promote the generation of CD11c+CD11b+B220− myeloid DCs, block completely the development of IPCs. IPCs generated display similar features to human IPCs, such as the plasmacytoid morphology, the ability to produce large amounts of IFN-α in responses to herpes simplex virus, and the capacity to respond to ligands for Toll-like receptor 9 (TLR-9; CpG ODN 1668), but not to ligands for TLR-4 (lipopolysaccharide [LPS]). Unlike human IPCs which produce little IL-12p70, mouse IPCs produce IL-12p70 in response to CpG ODN 1668 and herpes simplex virus. This study demonstrates that the development of murine CD11c+CD11b−B220+Gr-1+ IPCs and CD11c+CD11b+B220− myeloid DCs is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor. Human IPCs and mouse IPCs display different ability to produce IL-12p70. Large numbers of mouse IPCs can now be obtained from total bone marrow culture.

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