D3 dopamine receptor mRNA expression in lymphocytes: a peripheral marker for schizophrenia?

Background:Identification of schizophrenia, a common neuropsychiatric disorder, is based on clinical examination. An easily measurable peripheral marker, which may enable a more rapid and more accurate diagnosis, is not available. A possible candidate is the D3 dopamine receptor on lymphocytes.Objective:The D3 receptor is investigated for its clinical significance as a marker for diagnosing schizophrenia.Methods:From eight schizophrenic patients and eight controls lymphocyte RNA was isolated. A semiquantitative reverse transcription–polymerase chain reaction (RT-PCR) was carried out and the intensities of the specific D3 dopamine receptor bands of patients and controls were compared.Results:No difference could be seen between the intensities of the bands from patients and controls.Conclusion:An aberrant D3 dopamine receptor mRNA expression in lymphocytes of schizophrenics could not be demonstrated. This might be caused by down-regulation of D3 receptor production by antipsychotic drug treatment. At present, the D3 receptor seems to have no diagnostic value in schizophrenia.

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Oxytocin and oxytocin-receptor mRNA expression in the human gastrointestinal tract: a polymerase chain reaction study

Regulatory Peptides ◽

10.1016/j.regpep.2003.12.017 ◽

2004 ◽

Vol 119 (1-2) ◽

pp. 39-44 ◽

Cited By ~ 55

Author(s):

Hans-Jürg Monstein ◽

Niclas Grahn ◽

Mikael Truedsson ◽

Bodil Ohlsson

Keyword(s):

Polymerase Chain Reaction ◽

Gastrointestinal Tract ◽

Mrna Expression ◽

Oxytocin Receptor ◽

Human Gastrointestinal Tract ◽

Chain Reaction ◽

Reaction Study ◽

Receptor Mrna ◽

Polymerase Chain

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Evidence for dopamine D2 receptor mRNA expression by striatal astrocytes in culture: in situ hybridization and polymerase chain reaction studies

Molecular Brain Research ◽

10.1016/0169-328x(94)90227-5 ◽

1994 ◽

Vol 23 (3) ◽

pp. 204-212 ◽

Cited By ~ 62

Author(s):

Anna Bal ◽

Thomas Bachelot ◽

Marc Savasta ◽

Monique Manier ◽

Jean Marc Verna ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

In Situ Hybridization ◽

Mrna Expression ◽

Dopamine D2 Receptor ◽

D2 Receptor ◽

Chain Reaction ◽

Receptor Mrna ◽

Dopamine D2 ◽

Polymerase Chain

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Change of dopamine receptor mRNA expression in lymphocyte of schizophrenic patients

BMC Medical Genetics ◽

10.1186/1471-2350-2-3 ◽

2001 ◽

Vol 2 (1) ◽

Cited By ~ 58

Author(s):

Yong T Kwak ◽

Min-Seong Koo ◽

Chul-Hee Choi ◽

IN Sunwoo

Keyword(s):

Mrna Expression ◽

Dopamine Receptor ◽

Receptor Mrna ◽

Schizophrenic Patients

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Gene expressions of TS and MDR-1 are predictive factors for chemosensitivity and survival in esophageal cancer with fluoropyrimidine-based chemotherapy

Journal of Clinical Oncology ◽

10.1200/jco.2007.25.18_suppl.14120 ◽

2007 ◽

Vol 25 (18_suppl) ◽

pp. 14120-14120

Author(s):

K. Uchida ◽

K. Hayashi ◽

K. Kudo ◽

H. Kuramochi ◽

M. Yamamoto

Keyword(s):

Esophageal Cancer ◽

Mrna Expression ◽

Overall Response Rate ◽

Response Rate ◽

Rt Pcr ◽

Chain Reaction ◽

Gene Expressions ◽

Polymerase Chain ◽

Relative Mrna Expression ◽

Mdr 1

14120 Background: Fluoropyrimidines are widely used in chemotherapy regimens for esophageal cancer. To test the hypotheses of whether the relative mRNA expression of the enzyme TS, DPD and MDR-1 were associated with response to in esophageal cancer. We investigated the associations reported between intratumoral TS, DPD and MDR-1 gene expressions and the response with 5-FU based chemotherapy for patients with esophageal cancer. Methods: Twenty six pts with esophageal cancer were treated with 5-FU based chemotherapy (14 pts were treated with chemotherapy only, and 12 pts were chemoradiotherapy). mRNA was isolated from frozen tumor specimens, and relative expression levels of each gene/β-actin were measured using a quantitative reverse transcription polymerase chain reaction (RT-PCR) (Taqman®) system. Results: The overall response rate was 50.0%. Each mRNA expression was detectable in 26 patients. TS expressions were significantly lower in the responding tumors compared to the non-responders respectively (P=0.009). There were no significant associations between MDR-1 expression and the response. By setting up a cut-off level for TS and MDR-1 of Median, combining the two gene expression two-dimensionally revealed a relationship with the response (P=0.037). Additionally, MDR-1 expressions were statistically significant predictors of prolonged survival (P=0.0084). Conclusions: These results suggest that intratumoral TS and MDR-1 expressions are prognostic factors for survival after 5-FU based chemotherapy in esophageal cancer. No significant financial relationships to disclose.

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