Relationship between DNA content of polytene chromosome bands and heterochromatisation in Drosophila

Nature ◽  
1974 ◽  
Vol 248 (5443) ◽  
pp. 55-57 ◽  
Author(s):  
J. M. WARGENT ◽  
I. J. HARTMANN-GOLDSTEIN ◽  
D. J. GOLDSTEIN
Genetics ◽  
1980 ◽  
Vol 95 (1) ◽  
pp. 95-110 ◽  
Author(s):  
Arthur J Hilliker ◽  
Stephen H Clark ◽  
Arthur Chovnick ◽  
William M Gelbart

ABSTRACT This report describes the genetic analysis of a region of the third chromosome of Drosophila melanogaster extending from 87D2-4 to 87E12-F1, an interval of 23 or 24 polytene chromosome bands. This region includes the rosy (ry, 3-52.0) locus, carrying the structural information for xanthine dehydrogenase (XDH). We have, in recent years, focused attention on the genetic regulation of the rosy locus and, therefore, wished to ascertain in detail the immediate genetic environmcnt of this locus. Specifically, we question if rosy is a solitary genetic unit or part of a larger complex genetic unit encompassing adjacent genes. Our data also provide opportunity to examine further the relationship between euchromatic gene distrihution and polytene chromosome structure.—The results of our genetic dissection of the rosy microregion substantiate the conclusion drawn earlier (SCHALET, KERNAGHAN and CHOVNICK 1964) that the rosy locus is the only gene in this region concerned with XDH activity and that all adjacent genetic units are functionally, as well as spatially, distinct Erom the rosy gene. Within the rosy micro-region, we observed a close correspondence between the number of complementation groups (21) and the number of polytene chromosome bands (23 or 24). Consideration of this latter observation in conjunction with those of similar studies of other chhromosomal regions supports the hypothesis that each polytene chromosome band corresponds to a single genetic unit.


Genetics ◽  
1982 ◽  
Vol 102 (1) ◽  
pp. 71-74
Author(s):  
David J Broderick ◽  
Paul A Roberts

ABSTRACT Overlapping duplications recovered as suppressors of Minute loci have been used to localize M(2)z and M(3)w  124 to specific polytene bands 25A1(2) and 95A1(2). The surprising efficiency of M localization by duplication may result from the tendency of M suppressors to be at least a visible fraction of a polytene band in length.


1997 ◽  
Vol 17 (4) ◽  
pp. 2291-2300 ◽  
Author(s):  
M V Murray ◽  
M A Turnage ◽  
K J Williamson ◽  
W R Steinhauer ◽  
L L Searles

Mutations of the Drosophila melanogaster suppressor of sable [su(s)] gene, which encodes a 150-kDa nuclear protein [Su(s)], increase the accumulation of specific transcripts in a manner that is not well understood but that appears to involve pre-mRNA processing. Here, we report biochemical analysis of purified, recombinant Su(s) [rSu(s)] expressed in baculovirus and in Escherichia coli as maltose binding protein (MBP) fusions and immunocytochemical analysis of endogenous Su(s). This work has shown that purified, baculovirus-expressed rSu(s) binds to RNA in vitro with a high affinity and limited specificity. Systematic evolution of ligands by exponential enrichment was used to identify preferred RNA targets of rSu(s), and a large proportion of RNAs isolated contain a full or partial match to the consensus sequence UCAGUAGUCU, which was confirmed to be a high-affinity rSu(s) binding site. An MBP-Su(s) fusion protein containing the N-terminal third of Su(s) binds RNAs containing this sequence with a higher specificity than full-length, baculovirus-expressed rSu(s). The consensus sequence resembles both a cryptic 5' splice site and a sequence that is found near the 5' end of some Drosophila transcripts. Immunolocalization studies showed that endogenous Su(s) is distributed in a reticulated pattern in Drosophila embryo and salivary gland nuclei. In salivary gland cells, Su(s) is found both in the nucleoplasm and in association with a subset of polytene chromosome bands. Considering these and previous results, we propose two models to explain how su(s) mutations affect nuclear pre-mRNA processing.


Genetics ◽  
1979 ◽  
Vol 91 (3) ◽  
pp. 443-453
Author(s):  
Pedro Ripoll ◽  
A García-Bellido

ABSTRACT The viability of cells made homozygous for different deficiencies by induced mitotic recombination was examined. The deficiencies varied in length from two to 30 polytene chromosome bands and were distributed over the five major chromosome arms. Among a sample of 30, ten deficiencies were cell viable. Our results show that 12% of the genome is necessary for cell survival, supporting previous estimates of about 5,000 genes in the genome of Drosophila.


1981 ◽  
Vol 11 (4) ◽  
pp. 303-340 ◽  
Author(s):  
Igor F. Zhimulev ◽  
Elena S. Belyaeva ◽  
Valery F. Semeshin ◽  
M. Ashburner

2016 ◽  
Vol 466 (1) ◽  
pp. 57-60 ◽  
Author(s):  
I. F. Zhimulev ◽  
L. V. Boldyreva ◽  
O. V. Demakova ◽  
G. V. Poholkova ◽  
V. A. Khoroshko ◽  
...  

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