Differential staining of polytene chromosome bands in Chironomus by Giemsa banding methods

Chromosoma ◽  
1977 ◽  
Vol 59 (3) ◽  
pp. 207-216 ◽  
Author(s):  
Klaus H�gele
Keyword(s):  
Polytene Chromosome ◽  
Differential Staining ◽  
Giemsa Banding ◽  
Chromosome Bands

scholarly journals CYTOGENETIC ANALYSIS OF THE CHROMOSOMAL REGION IMMEDIATELY ADJACENT TO THE ROSY LOCUS IN DROSOPHILA MELANOGASTER

Genetics ◽  
1980 ◽  
Vol 95 (1) ◽  
pp. 95-110 ◽  
Author(s):  
Arthur J Hilliker ◽  
Stephen H Clark ◽  
Arthur Chovnick ◽  
William M Gelbart
Keyword(s):  
Drosophila Melanogaster ◽  
Polytene Chromosome ◽  
Structural Information ◽  
Genetic Regulation ◽  
Chromosome Band ◽  
Genetic Dissection ◽  
Genetic Unit ◽  
Complementation Groups ◽  
The Relationship ◽  
Chromosome Bands

ABSTRACT This report describes the genetic analysis of a region of the third chromosome of Drosophila melanogaster extending from 87D2-4 to 87E12-F1, an interval of 23 or 24 polytene chromosome bands. This region includes the rosy (ry, 3-52.0) locus, carrying the structural information for xanthine dehydrogenase (XDH). We have, in recent years, focused attention on the genetic regulation of the rosy locus and, therefore, wished to ascertain in detail the immediate genetic environmcnt of this locus. Specifically, we question if rosy is a solitary genetic unit or part of a larger complex genetic unit encompassing adjacent genes. Our data also provide opportunity to examine further the relationship between euchromatic gene distrihution and polytene chromosome structure.—The results of our genetic dissection of the rosy microregion substantiate the conclusion drawn earlier (SCHALET, KERNAGHAN and CHOVNICK 1964) that the rosy locus is the only gene in this region concerned with XDH activity and that all adjacent genetic units are functionally, as well as spatially, distinct Erom the rosy gene. Within the rosy micro-region, we observed a close correspondence between the number of complementation groups (21) and the number of polytene chromosome bands (23 or 24). Consideration of this latter observation in conjunction with those of similar studies of other chhromosomal regions supports the hypothesis that each polytene chromosome band corresponds to a single genetic unit.

scholarly journals LOCALIZATION OF MINUTES TO SPECIFIC POLYTENE CHROMOSOME BANDS BY MEANS OF OVERLAPPING DUPLICATIONS

Genetics ◽  
1982 ◽  
Vol 102 (1) ◽  
pp. 71-74
Author(s):  
David J Broderick ◽  
Paul A Roberts
Keyword(s):  
Polytene Chromosome ◽  
Chromosome Bands

ABSTRACT Overlapping duplications recovered as suppressors of Minute loci have been used to localize M(2)z and M(3)w  124 to specific polytene bands 25A1(2) and 95A1(2). The surprising efficiency of M localization by duplication may result from the tendency of M suppressors to be at least a visible fraction of a polytene band in length.

Relationship between DNA content of polytene chromosome bands and heterochromatisation in Drosophila

Nature ◽  
1974 ◽  
Vol 248 (5443) ◽  
pp. 55-57 ◽  
Author(s):  
J. M. WARGENT ◽  
I. J. HARTMANN-GOLDSTEIN ◽  
D. J. GOLDSTEIN
Keyword(s):  
Polytene Chromosome ◽  
Dna Content ◽  
Chromosome Bands

A combination of sister chromatid differential staining and giemsa banding

1975 ◽  
Vol 31 (8) ◽  
pp. 916-918 ◽  
Author(s):  
S. Pathak ◽  
A. D. Stock ◽  
A. Lusby
Keyword(s):  
Sister Chromatid ◽  
Differential Staining ◽  
Giemsa Banding

Chromosome pairing failure in an intersectional amphiploid of Bromus altissimus × B. arvensis

1985 ◽  
Vol 27 (6) ◽  
pp. 705-709 ◽  
Author(s):  
K. C. Armstrong
Keyword(s):  
Chromosome Pairing ◽  
Embryo Culture ◽  
Differential Staining ◽  
Root Tip ◽  
Giemsa Banding ◽  
Homologous Pairing ◽  
Hybrid Nature ◽  
Tip Cells ◽  
Root Tip Cells

An intersectional F1 hybrid between Bromus arvensis and B. altissimus was made with the aid of embryo culture. The hybrid nature of the F1 and the amphiploid were confirmed by karyotyping root-tip cells. Following Giemsa banding, the chromosomes of B. arvensis stained darker than those of B. altissimus. Very little chromosome pairing was observed in the F1 hybrid (11.60 I + 1.08 II + 0.10 III). Chromosome pairing in the amphiploid (2n = 28) varied from almost complete pairing to very little pairing in different samples. The chromosome pairing indicated that very little homology exists between the genomes of B. altissimus and B. arvensis. Pairing failure in the amphiploid may result from the action of pairing control genes which are strong enough to prevent homologous pairing but which are variable in expression because of a sensitivity to endogenous and exogenous factors.Key words: Bromus, amphiploid, chromosome pairing, differential staining.

scholarly journals Chromosomal Abnormalities in Endometrial and Ovarian Carcinomas

2007 ◽  
Vol 10 (2) ◽  
pp. 61-70 ◽  
Author(s):  
A Pazarbaşi ◽  
M Kasap ◽  
O Demirhan ◽  
M Vardar ◽  
D Suleymanova-Karahan ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Chromosomal Abnormalities ◽  
Banding Technique ◽  
Giemsa Banding ◽  
Specific Chromosome ◽  
Ovarian Carcinomas ◽  
Genetic Changes ◽  
Cytogenetic Studies ◽  
Chromosomal Instabilities ◽  
Chromosome Bands

Chromosomal Abnormalities in Endometrial and Ovarian CarcinomasDevelopment and progression of human malignancies involve multiple genetic changes including chromosomal instabilities such as translocations, deletions, and inversions. Chromosomal abnormalities were observed in 23 cases with ovarian and endometrial cancer by cytogenetic studies using a GTG (G bands by trypsin using Giemsa) banding technique. Specific chromosome bands were frequently involved, and were most frequent on chromosomes 1, 2, 3, 5, 12 and 17. Clonal alterations were observed at the cancer breakpoints, such as 1q21, 1q32, 3p21, 7q22, 11q23 in ovarian and 1p36, 1q32, 2p12, 3p21, 7q22, 9q34, 11p15, 11q23, 12q13, 14q11, 14q32, 16p13, 21q22 in endometrial cases. These findings provide evidence that multiple genetic lesions are associated with the pathogenesis of endometrial and ovarian cancer.

scholarly journals The Drosophila suppressor of sable protein binds to RNA and associates with a subset of polytene chromosome bands.

1997 ◽  
Vol 17 (4) ◽  
pp. 2291-2300 ◽  
Author(s):  
M V Murray ◽  
M A Turnage ◽  
K J Williamson ◽  
W R Steinhauer ◽  
L L Searles
Keyword(s):  
Salivary Gland ◽  
Polytene Chromosome ◽  
Consensus Sequence ◽  
Mrna Processing ◽  
Drosophila Embryo ◽  
High Affinity ◽  
Suppressor Of Sable ◽  
Exponential Enrichment ◽  
Chromosome Bands

Mutations of the Drosophila melanogaster suppressor of sable [su(s)] gene, which encodes a 150-kDa nuclear protein [Su(s)], increase the accumulation of specific transcripts in a manner that is not well understood but that appears to involve pre-mRNA processing. Here, we report biochemical analysis of purified, recombinant Su(s) [rSu(s)] expressed in baculovirus and in Escherichia coli as maltose binding protein (MBP) fusions and immunocytochemical analysis of endogenous Su(s). This work has shown that purified, baculovirus-expressed rSu(s) binds to RNA in vitro with a high affinity and limited specificity. Systematic evolution of ligands by exponential enrichment was used to identify preferred RNA targets of rSu(s), and a large proportion of RNAs isolated contain a full or partial match to the consensus sequence UCAGUAGUCU, which was confirmed to be a high-affinity rSu(s) binding site. An MBP-Su(s) fusion protein containing the N-terminal third of Su(s) binds RNAs containing this sequence with a higher specificity than full-length, baculovirus-expressed rSu(s). The consensus sequence resembles both a cryptic 5' splice site and a sequence that is found near the 5' end of some Drosophila transcripts. Immunolocalization studies showed that endogenous Su(s) is distributed in a reticulated pattern in Drosophila embryo and salivary gland nuclei. In salivary gland cells, Su(s) is found both in the nucleoplasm and in association with a subset of polytene chromosome bands. Considering these and previous results, we propose two models to explain how su(s) mutations affect nuclear pre-mRNA processing.

scholarly journals VIABILITY OF HOMOZYGOUS DEFICIENCIES IN SOMATIC CELLS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1979 ◽  
Vol 91 (3) ◽  
pp. 443-453
Author(s):  
Pedro Ripoll ◽  
A García-Bellido
Keyword(s):  
Drosophila Melanogaster ◽  
Cell Survival ◽  
Polytene Chromosome ◽  
Somatic Cells ◽  
Mitotic Recombination ◽  
Chromosome Bands

ABSTRACT The viability of cells made homozygous for different deficiencies by induced mitotic recombination was examined. The deficiencies varied in length from two to 30 polytene chromosome bands and were distributed over the five major chromosome arms. Among a sample of 30, ten deficiencies were cell viable. Our results show that 12% of the genome is necessary for cell survival, supporting previous estimates of about 5,000 genes in the genome of Drosophila.

Informational Content of Polytene Chromosome Bands and Puffs

1981 ◽  
Vol 11 (4) ◽  
pp. 303-340 ◽  
Author(s):  
Igor F. Zhimulev ◽  
Elena S. Belyaeva ◽  
Valery F. Semeshin ◽  
M. Ashburner
Keyword(s):  
Polytene Chromosome ◽  
Informational Content ◽  
Chromosome Bands
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