Fucosylglycolipids in cells transformed by a temperature-sensitive mutant of murine sarcoma virus

Nature ◽  
1974 ◽  
Vol 248 (5450) ◽  
pp. 682-684 ◽  
Author(s):  
SHELDON M. STEINER ◽  
JOSEPH L. MELNICK ◽  
SAUL KIT ◽  
KENNETH D. SOMERS
Keyword(s):  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells

scholarly journals Dissociation between transformed and differentiated phenotype in rat thyroid epithelial cells after transformation with a temperature-sensitive mutant of the Kirsten murine sarcoma virus.

1983 ◽  
Vol 3 (11) ◽  
pp. 2099-2109 ◽  
Author(s):  
G Colletta ◽  
A Pinto ◽  
P P Di Fiore ◽  
A Fusco ◽  
M Ferrentino ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Functional Markers ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Phenotypic Properties ◽  
Temperature Sensitive Mutant ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Rat Thyroid ◽  
Murine Sarcoma

Differentiated rat thyroid epithelial cells, infected in vitro with a temperature-sensitive mutant of the Kirsten murine sarcoma virus, expressed at the permissive temperature (33 degrees C) some phenotypic properties typical of transformed cells, including morphological features, colony formation in agar, and induction of tumors in newborn animals. Specific functional markers of these differentiated cells, i.e., synthesis/secretion of thyroglobulin, synthesis of thyroglobulin mRNA and iodide uptake, were blocked during growth at 33 degrees C. Normal morphology, failure to grow in agar, and the requirement of hormones for optimal growth were all restored after shifting to the temperature nonpermissive for transformation (39 degrees C), though the typical differentiated functions remained blocked. Infection with a leukemia helper virus clone (Moloney or Kirsten murine leukemia virus) did not lead to the loss of the differentiated phenotype of rat epithelial thyroid cells, thus demonstrating that the loss of the differentiated phenotype is caused by the sarcoma virus component. These results indicate that the expression of some of the phenotypic properties of transformed differentiated rat thyroid epithelial cells is under the direct control of the p21 thermosensitive activity, whereas the block in the expression of two typical differentiation markers of thyroid epithelial cells is irreversible and probably controlled by different mechanisms.

Dissociation between transformed and differentiated phenotype in rat thyroid epithelial cells after transformation with a temperature-sensitive mutant of the Kirsten murine sarcoma virus

1983 ◽  
Vol 3 (11) ◽  
pp. 2099-2109
Author(s):  
G Colletta ◽  
A Pinto ◽  
P P Di Fiore ◽  
A Fusco ◽  
M Ferrentino ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Functional Markers ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Phenotypic Properties ◽  
Temperature Sensitive Mutant ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Rat Thyroid ◽  
Murine Sarcoma

Differentiated rat thyroid epithelial cells, infected in vitro with a temperature-sensitive mutant of the Kirsten murine sarcoma virus, expressed at the permissive temperature (33 degrees C) some phenotypic properties typical of transformed cells, including morphological features, colony formation in agar, and induction of tumors in newborn animals. Specific functional markers of these differentiated cells, i.e., synthesis/secretion of thyroglobulin, synthesis of thyroglobulin mRNA and iodide uptake, were blocked during growth at 33 degrees C. Normal morphology, failure to grow in agar, and the requirement of hormones for optimal growth were all restored after shifting to the temperature nonpermissive for transformation (39 degrees C), though the typical differentiated functions remained blocked. Infection with a leukemia helper virus clone (Moloney or Kirsten murine leukemia virus) did not lead to the loss of the differentiated phenotype of rat epithelial thyroid cells, thus demonstrating that the loss of the differentiated phenotype is caused by the sarcoma virus component. These results indicate that the expression of some of the phenotypic properties of transformed differentiated rat thyroid epithelial cells is under the direct control of the p21 thermosensitive activity, whereas the block in the expression of two typical differentiation markers of thyroid epithelial cells is irreversible and probably controlled by different mechanisms.

A selective temperature-sensitive defect in viral RNA expression in cells infected with a ts transformation mutant of murine sarcoma virus

Cell ◽  
1981 ◽  
Vol 25 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Jacqueline Peltier Horn ◽  
T.Gordon Wood ◽  
Edwin C. Murphy ◽  
Donald G. Blair ◽  
Ralph B. Arlinghaus
Keyword(s):  
Viral Rna ◽  
Temperature Sensitive ◽  
Rna Expression ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells

Modification of gap junctions in cells transformed by a temperature-sensitive mutant of Rous sarcoma virus

1986 ◽  
Vol 91 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Michael M. Atkinson ◽  
Susan K. Anderson ◽  
Judson D. Sheridan
Keyword(s):  
Gap Junctions ◽  
Rous Sarcoma Virus ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
In Cells

Characterization of the Major Viral Polypeptide in Cells Transformed by Wild-Type and Temperature-Sensitive Murine Sarcoma Virus

Intervirology ◽  
1974 ◽  
Vol 4 (2) ◽  
pp. 99-109
Author(s):  
Gad Spira ◽  
Gordon R. Dreesman ◽  
Matilda Benyesh-Melnick ◽  
Saul Kit ◽  
Kenneth D. Somers
Keyword(s):  
Temperature Sensitive ◽  
Wild Type ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells

scholarly journals Expression of the Major Internal Viral Polypeptide in Cells Transformed by Wild-Type and Temperature-Sensitive Murine Sarcoma Virus

1974 ◽  
Vol 14 (5) ◽  
pp. 1245-1252 ◽  
Author(s):  
Gad Spira ◽  
Gordon R. Dreesman ◽  
Matilda Benyesh-Melnick ◽  
Saul Kit ◽  
Kenneth D. Somers
Keyword(s):  
Temperature Sensitive ◽  
Wild Type ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells
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