Characterization of the Major Viral Polypeptide in Cells Transformed by Wild-Type and Temperature-Sensitive Murine Sarcoma Virus

Intervirology ◽  
1974 ◽  
Vol 4 (2) ◽  
pp. 99-109
Author(s):  
Gad Spira ◽  
Gordon R. Dreesman ◽  
Matilda Benyesh-Melnick ◽  
Saul Kit ◽  
Kenneth D. Somers
Keyword(s):  
Temperature Sensitive ◽  
Wild Type ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells

scholarly journals Expression of the Major Internal Viral Polypeptide in Cells Transformed by Wild-Type and Temperature-Sensitive Murine Sarcoma Virus

1974 ◽  
Vol 14 (5) ◽  
pp. 1245-1252 ◽  
Author(s):  
Gad Spira ◽  
Gordon R. Dreesman ◽  
Matilda Benyesh-Melnick ◽  
Saul Kit ◽  
Kenneth D. Somers
Keyword(s):  
Temperature Sensitive ◽  
Wild Type ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells

A selective temperature-sensitive defect in viral RNA expression in cells infected with a ts transformation mutant of murine sarcoma virus

Cell ◽  
1981 ◽  
Vol 25 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Jacqueline Peltier Horn ◽  
T.Gordon Wood ◽  
Edwin C. Murphy ◽  
Donald G. Blair ◽  
Ralph B. Arlinghaus
Keyword(s):  
Viral Rna ◽  
Temperature Sensitive ◽  
Rna Expression ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells

Fucosylglycolipids in cells transformed by a temperature-sensitive mutant of murine sarcoma virus

Nature ◽  
1974 ◽  
Vol 248 (5450) ◽  
pp. 682-684 ◽  
Author(s):  
SHELDON M. STEINER ◽  
JOSEPH L. MELNICK ◽  
SAUL KIT ◽  
KENNETH D. SOMERS
Keyword(s):  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells

scholarly journals Characterization of Viral RNA in Cells Transformed by Various Isolates of Moloney Murine Sarcoma Virus

1983 ◽  
Vol 64 (9) ◽  
pp. 2057-2062 ◽  
Author(s):  
R. Hamelin ◽  
J. Devaux ◽  
N. Honore ◽  
M.-A. Auger-Buendia ◽  
A. Tavitian
Keyword(s):  
Viral Rna ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Moloney Murine Sarcoma Virus ◽  
Murine Sarcoma ◽  
In Cells

Altered junctional permeability between cells transformed by v-ras, v-mos, or v-src

1988 ◽  
Vol 255 (5) ◽  
pp. C674-C683 ◽  
Author(s):  
M. M. Atkinson ◽  
J. D. Sheridan
Keyword(s):  
Cell Junctions ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Cell Pair ◽  
Rous Sarcoma ◽  
Murine Sarcoma Virus ◽  
Junctional Permeability ◽  
Sarcoma Virus ◽  
A Cell ◽  
Murine Sarcoma

Junctional permeability in normal and transformed NRK cells was quantitatively assessed by microinjecting fluorescent dye into one cell of a pair, digitizing the changes of fluorescence intensity using video analysis techniques, and applying the digital values to a solution of Fick's diffusion equation. We show that this approach reliably estimates the junctional permeance of a cell pair. Cells that are temperature sensitive for transformation were shown to also be temperature sensitive vis-a-vis junctional permeance. Thus permeance values were reduced approximately 80-90% on transformation by either a mutant Rous sarcoma virus or a mutant Moloney murine sarcoma virus. Cells transformed by wild-type Kirsten sarcoma virus were also shown to possess levels of junctional permeance significantly lower than nontransformed controls. The transformed junctional phenotype could be observed as early as 15 min after shifting to transformation-permissive conditions. Our results suggest that the oncogenes src, ras, and mos exert their effects on NRK cell junctions via converging pathways, of which one may be phosphorylation of junctional proteins.

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