Antipyrine elimination and hepatic microsomal enzyme activity in patients with liver disease

1. Albino rats were fed on diets containing 30, 120 or 200 g protein/kg with or without the incorporation of dichlorodiphenyltrichloroethane (DDT) or hexachlorocyclohexane (HCH) at 100 mg/kg diet for 4 weeks.2. The activities of the liver microsomal enzymes NADPH-cytochrome reductase (EC 1.6.2.4), flavoprotein- linked monooxygenase (EC 1.14.14.1)and O-demethylase were significantly greater in animals fed on 120 and 200 g protein/kg diet compared with those fed on 30 g protein/kg diet.3. The inclusion of DDT or HCH at all protein intakes led to further significant rises in microsomal enzyme activities but the increases were much greater for animals receiving the 120 and 200 g protein/kg diets than for those receiving the 30 g protein/kg diet.4. The results imply that detoxification of DDT or HCH was carried out more effectively at the higher protein intakes.

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Prevention of the acute ethanol-induced fatty liver by antihistamines and stimulants of hepatic microsomal enzyme activity

Toxicology and Applied Pharmacology ◽

10.1016/0041-008x(68)90030-6 ◽

1968 ◽

Vol 12 (2) ◽

pp. 186-193 ◽

Cited By ~ 8

Author(s):

W.R. Wooles

Keyword(s):

Enzyme Activity ◽

Fatty Liver ◽

Microsomal Enzyme ◽

Hepatic Microsomal Enzyme ◽

Acute Ethanol

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Effect of cannabichromene on hepatic microsomal enzyme activity in the mouse

General Pharmacology The Vascular System ◽

10.1016/0306-3623(83)90044-7 ◽

1983 ◽

Vol 14 (3) ◽

pp. 361-363 ◽

Cited By ~ 5

Author(s):

J.C. Kapeghian ◽

A.B. Jones ◽

J.C. Murphy ◽

M.A. Elsohly ◽

C.E. Turner

Keyword(s):

Enzyme Activity ◽

Microsomal Enzyme ◽

Hepatic Microsomal Enzyme

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Increased D-glucaric acid excretion by jaundiced patients.

Clinical Chemistry ◽

10.1093/clinchem/24.10.1759 ◽

1978 ◽

Vol 24 (10) ◽

pp. 1759-1761 ◽

Cited By ~ 3

Author(s):

M Herzberg ◽

M H Wiener

Keyword(s):

Enzyme Activity ◽

Liver Disease ◽

Obstructive Jaundice ◽

Glucuronic Acid ◽

Liver Function Tests ◽

Acid Excretion ◽

Glucaric Acid ◽

Gamma Glutamyltransferase ◽

Hepatic Microsomal Enzyme ◽

Udp Glucuronosyltransferase

Abstract The urinary excretion of D-glucaric acid, a catabolite of glucuronic acid, is considered to be a reliable index of the state of hepatic microsomal enzyme activity. Because enzyme activity may be altered in liver disease, we examined the effect of liver disease on the excretion of this metabolite and its correlation with liver function tests. We studied 89 patients with nonhemolytic jaundice, 39 with viral hepatitis, 33 with obstructive jaundice, six with cirrhosis, and 11 patients with jaundice of mixed etiology. Glucaric acid excretion was significantly increased in all these patients as compared to controls, most pronounced in the obstructive jaundice group. No correlation was found between glucaric acid excretion and concentrations of bilirubin, albumin, globulin, aspartate aminotransferase, alkaline phosphatase, cholesterol, or gamma-glutamyltransferase in serum, even though the concentrations of these analytes did vary with the type of liver disease. We suggest that this increase in glucaric acid excretion is an indication of normal or even increased glucuronidation (UDP-glucuronosyltransferase activity), which occurs in liver disease.

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