Functional analysis of macrophages, B cells and splenic dendritic cells as antigen-presenting cells in West Nile virus-specific murine T lymphocyte proliferation

1991 ◽  
Vol 69 (2) ◽  
pp. 71-80 ◽  
Author(s):  
A. B. KULKARNI ◽  
A. MÜLLBACHER ◽  
R. V. BLANDEN
Keyword(s):  
Functional Analysis ◽  
Dendritic Cells ◽  
West Nile Virus ◽  
B Cells ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Antigen Presenting Cells ◽  
West Nile ◽  
T Lymphocyte Proliferation ◽  
Antigen Presenting

scholarly journals CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4+T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Tomasz Maj ◽  
Anna Slawek ◽  
Anna Chelmonska-Soyta
Keyword(s):  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Costimulatory Molecule ◽  
Antigen Presenting Cells ◽  
Costimulatory Molecules ◽  
Cytokine Response ◽  
Cytokine Balance ◽  
T Lymphocyte Proliferation ◽  
Antigen Presenting

Immune phenomena during the preimplantation period of pregnancy are poorly understood. The aim of our study was to assess the capacity for antigen presentation of splenic antigen-presenting cells (APCs) derived from pregnant and pseudopregnant mice inin vitroconditions. Therefore, sorted CD11c+dendritic cells and macrophages F4/80+and CD11b+presenting ovalbumin (OVA) were cocultured with CD4+T cells derived from OT-II mice’s (C57BL6/J-Tg(TcraTcrb)1100Mjb/J) spleen. After 132 hours of cell culture, proliferation of lymphocytes (ELISA-BrdU), activation of these cells (flow cytometry), cytokine profile (ELISA), and influence of costimulatory molecules blocking on these parameters were measured. We did not detect any differences in regulation of Th1/Th2 cytokine balance. CD86 seems to be the main costimulatory molecule involved in the proliferation response but CD80 is the main costimulatory molecule influencing cytokine secretion in pregnant mice. In conclusion, this study showed that CD80 and CD86 costimulatory molecules regulate OT-II CD4+T lymphocyte proliferation and cytokine response in cocultures with antigen-presenting cells derived from pregnant and pseudopregnant mice. The implications of these changes still remain unclear.

scholarly journals Follicular dendritic cells help resting B cells to become effective antigen-presenting cells: induction of B7/BB1 and upregulation of major histocompatibility complex class II molecules.

1993 ◽  
Vol 178 (6) ◽  
pp. 2055-2066 ◽  
Author(s):  
M H Kosco-Vilbois ◽  
D Gray ◽  
D Scheidegger ◽  
M Julius
Keyword(s):  
Dendritic Cells ◽  
Major Histocompatibility Complex ◽  
B Cells ◽  
Major Histocompatibility Complex Class ◽  
B Cell ◽  
Antigen Presenting Cells ◽  
Follicular Dendritic Cells ◽  
Complex Class ◽  
Histocompatibility Complex ◽  
Antigen Presenting

This study was designed to investigate whether follicular dendritic cells (FDC) can activate B cells to a state in which they can function as effective antigen-presenting cells (APC). High buoyant density (i.e., resting) B cells specific for 2,4-dinitro-fluorobenzene (DNP) were incubated with DNP-ovalbumin (OVA) bearing FDC, after which their capacity to process and present to an OVA-specific T cell clone was assessed. The efficacies of alternative sources of antigen and activation signals in the induction of B cell APC function were compared with those provided by FDC. Only FDC and Sepharose beads coated with anti-immunoglobulin (Ig)kappa monoclonal antibody provided the necessary stimulus. FDC carrying inappropriate antigens also induced B cell APC function in the presence of exogenous DNP-OVA. However, in circumstances where soluble DNP-OVA was limiting, FDC bearing complexes containing DNP, which could crosslink B cell Ig receptors, induced the most potent APC function. Analysis by flow cytometry revealed that within 24 h of coculture with FDC, a significant percentage of B cells increased in size and expressed higher levels of major histocompatibility complex class II. By 48 h, an upregulation of the costimulatory molecule, B7/BB1, occurred, but only when exposed to the FDC bearing DNP. Taken together, the results demonstrate that FDC have the capacity to activate resting B cells to a state in which they can function as APC for T cells. The stimuli that FDC provide may include: (a) an antigen-dependent signal that influences the upregulation of B7/BB1; and (b) possibly a signal independent of crosslinking mIg that results in Ig internalization. The relevance of these findings to the formation of germinal centers and maintenance of the humoral response is discussed.

scholarly journals Lymphocyte recruitment into the aortic wall before and during development of atherosclerosis is partially L-selectin dependent

2006 ◽  
Vol 203 (5) ◽  
pp. 1273-1282 ◽  
Author(s):  
Elena Galkina ◽  
Alexandra Kadl ◽  
John Sanders ◽  
Danielle Varughese ◽  
Ian J. Sarembock ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Lymphocyte ◽  
Lymphoid Tissue ◽  
Lymphocyte Proliferation ◽  
Aortic Wall ◽  
Immune Cell ◽  
T Lymphocyte Proliferation ◽  
Deficient Mice ◽  
Progression Of Atherosclerosis ◽  
B And T Lymphocytes

Atherosclerosis is an inflammatory disease of large arteries. Flow cytometry of aortic cell suspensions showed that B and T lymphocytes and some macrophages and dendritic cells are already present in the adventitia of normal/noninflamed mouse aortas. Adoptively transferred lymphocytes constitutively homed to the aorta and resided within the adventitia up to 7 d after transfer. Lymphocyte trafficking into normal/noninflamed or atherosclerosis-prone aortas was partially L-selectin dependent. Antigen-activated dendritic cells induced increased T lymphocyte proliferation within the aorta 72 h after adoptive transfer. During progression of atherosclerosis in apolipoprotein-E–deficient mice, the total number of macrophages, T cells, and dendritic cells, but not B cells, increased significantly. This alteration in immune cell composition was accompanied by the formation of tertiary lymphoid tissue in the adventitia of atherosclerotic aortas. These results demonstrate that lymphocytes already reside within the normal/noninflamed aorta before the onset atherosclerosis as a consequence of constitutive trafficking. Atherosclerosis induces the recruitment of macrophages and dendritic cells that support antigen presentation.

Dysfunction and infection of freshly isolated blood myeloid and plasmacytoid dendritic cells in patients infected with HIV-1

Blood ◽  
2003 ◽  
Vol 101 (11) ◽  
pp. 4505-4511 ◽  
Author(s):  
Heather Donaghy ◽  
Brian Gazzard ◽  
Frances Gotch ◽  
Steven Patterson
Keyword(s):  
Dendritic Cells ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Antiretroviral Treatment ◽  
Secondary Infection ◽  
T Lymphocyte Proliferation ◽  
Treatment Naïve ◽  
Plasmacytoid Dcs ◽  
Polymerase Chain ◽  
Hiv 1

Abstract Recently it has been shown that the 2 populations of blood dendritic cells (DCs), termed plasmacytoid (pcDCs) and myeloid (myDCs), are reduced in HIV-1 infection. This study aimed to determine whether these 2 populations are targets for HIV-1 infection and whether their ability to stimulate T-lymphocyte proliferation is affected. Highly purified populations of myDCs and pcDCs were isolated from the blood of antiretroviral treatment–naive patients and assessed for the level of HIV provirus by polymerase chain reaction (PCR). We show that both populations are targets for HIV-1 infection as indicated by the presence of provirus in 12 of 14 pcDC and 13 of 14 myDC samples tested. A proportion of this provirus is integrated in myDCs. The ability of both myDCs and pcDCs from HIV-1–infected patients to stimulate allogeneic T-lymphocyte proliferation in a 6-day mixed leukocyte reaction was severely impaired, but was not mediated by secondary infection of T lymphocytes. Thus, in addition to depletion, both myeloid and plasmacytoid DCs are infected and show impaired functional capacity. These findings suggest that infection, depletion, and dysfunction of dendritic cells may contribute to the immunosuppression associated with HIV-1 disease.

Accessory cell dependent T lymphocyte proliferation: potent activity of dendritic cells

Immunobiology ◽  
1987 ◽  
Vol 174 (1) ◽  
pp. 30-42 ◽  
Author(s):  
Estelle M. Goodell ◽  
Janet K. Stoltenborg ◽  
W.E. Bowers
Keyword(s):  
Dendritic Cells ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Accessory Cell ◽  
T Lymphocyte Proliferation
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