Reprogramming of Human Hair Follicle Dermal Papilla Cells into Induced Pluripotent Stem Cells

In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintain proliferation and differentiation of the epithelial stem cells that produce the hair fibre. In view of their regulatory properties, in this study, we investigated the interaction between hair follicle dermal cells (DP and DS) and embryonic stem cells (ESCs); induced pluripotent stem cells (iPSCs); and haematopoietic stem cells. We found that coculture of follicular dermal cells with ESCs or iPSCs supported their prolonged maintenance in an apparently undifferentiated state as established by differentiation assays, immunocytochemistry, and RT-PCR for markers of undifferentiated ESCs. We further showed that cytokines that are involved in ESC support are also expressed by cultured follicle dermal cells, providing a possible explanation for maintenance of ES cell stemness in cocultures. The same cytokines were expressed within folliclesin situin a pattern more consistent with a role in follicle growth activities than stem cell maintenance. Finally, we show that cultured mouse follicle dermal cells provide good stromal support for haematopoiesis in an established coculture model. Human follicular dermal cells represent an accessible and readily propagated source of feeder cells for pluripotent and haematopoietic cells and have potential for use in clinical applications.

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Faculty Opinions recommendation of Oct4 and klf4 reprogram dermal papilla cells into induced pluripotent stem cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1497956.1046054 ◽

2010 ◽

Author(s):

Jiro Kishimoto

Keyword(s):

Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Dermal Papilla ◽

Dermal Papilla Cells ◽

Induced Pluripotent

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Induced pluripotent stem cells from human hair follicle keratinocytes as a potential source for in vitro hair follicle cloning

PeerJ ◽

10.7717/peerj.2695 ◽

2016 ◽

Vol 4 ◽

pp. e2695 ◽

Cited By ~ 6

Author(s):

Sheng Jye Lim ◽

Shu Cheow Ho ◽

Pooi Ling Mok ◽

Kian Lee Tan ◽

Alan H.K. Ong ◽

...

Keyword(s):

Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Hair Follicle ◽

Pluripotent Stem Cells ◽

Human Hair ◽

Hair Follicles ◽

Reverse Transcription Pcr ◽

Pluripotency Markers ◽

Induced Pluripotent

Background Human hair follicles are important for the renewal of new hairs and their development. The generation of induced pluripotent stem cells (iPSCs) from hair follicles is easy due to its accessibility and availability. The pluripotent cells derived from hair follicles not only have a higher tendency to re-differentiate into hair follicles, but are also more suited for growth in hair scalp tissue microenvironment. Methods In this study, human hair follicular keratinocytes were used to generate iPSCs, which were then further differentiated in vitro into keratinocytes. The derived iPSCs were characterised by using immunofluorescence staining, flow cytometry, and reverse-transcription PCR to check for its pluripotency markers expression. Results The iPSC clones expressed pluripotency markers such as TRA-1-60, TRA-1-81, SSEA4, OCT4, SOX2, NANOG, LEFTY, and GABRB. The well-formed three germ layers were observed during differentiation using iPSCs derived from hair follicles. The successful formation of keratioctyes from iPSCs was confirmed by the expression of cytokeratin 14 marker. Discussion Hair follicles represent a valuable keratinocytes source for in vitro hair cloning for use in treating hair balding or grafting in burn patients. Our significant findings in this report proved that hair follicles could be used to produce pluripotent stem cells and suggested that the genetic and micro-environmental elements of hair follicles might trigger higher and more efficient hair follicles re-differentiation.

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