scholarly journals Single-particle Tracking as a Quantitative Microscopy-based Approach to Unravel Cell Entry Mechanisms of Viruses and Pharmaceutical Nanoparticles

2011 ◽  
Vol 19 (7) ◽  
pp. 1199-1211 ◽  
Author(s):  
Nadia Ruthardt ◽  
Don C Lamb ◽  
Christoph Bräuchle
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Cell Entry ◽  
Quantitative Microscopy

scholarly journals Dissecting the Cell Entry Pathway of Dengue Virus by Single-Particle Tracking in Living Cells

2008 ◽  
Vol 4 (12) ◽  
pp. e1000244 ◽  
Author(s):  
Hilde M. van der Schaar ◽  
Michael J. Rust ◽  
Chen Chen ◽  
Heidi van der Ende-Metselaar ◽  
Jan Wilschut ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Particle Tracking ◽  
Single Particle ◽  
Living Cells ◽  
Single Particle Tracking ◽  
Cell Entry ◽  
Entry Pathway

Single-Particle Tracking and Modulation of Cell Entry Pathways of a Tetrahedral DNA Nanostructure in Live Cells

2014 ◽  
Vol 53 (30) ◽  
pp. 7745-7750 ◽  
Author(s):  
Le Liang ◽  
Jiang Li ◽  
Qian Li ◽  
Qing Huang ◽  
Jiye Shi ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Cell Entry ◽  
Live Cells ◽  
Dna Nanostructure

Single-Particle Tracking and Modulation of Cell Entry Pathways of a Tetrahedral DNA Nanostructure in Live Cells

2014 ◽  
Vol 126 (30) ◽  
pp. 7879-7884 ◽  
Author(s):  
Le Liang ◽  
Jiang Li ◽  
Qian Li ◽  
Qing Huang ◽  
Jiye Shi ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Cell Entry ◽  
Live Cells ◽  
Dna Nanostructure

scholarly journals Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy

Entropy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 498
Author(s):  
Chen Zhang ◽  
Kevin Welsher
Keyword(s):  
Fisher Information ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Samples ◽  
Tracking System ◽  
Single Particle Tracking ◽  
Uniform Sampling ◽  
Single Dimension ◽  
3D Localization ◽  
Particle Localization

In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling can be achieved when a similar off-center pattern is used in 3D localization. Here, we systematically investigated the photon efficiency of different emission patterns in a diffraction-limited system and achieved higher precision than uniform sampling. The ability to maximize information from the limited number of photons demonstrated here is critical for particle tracking applications in biological samples, where photons may be limited.

Sign in / Sign up

Export Citation Format

Share Document