Identification of a DNA primase template tracking site redefines the geometry of primer synthesis

2008 ◽  
Vol 15 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Jacob E Corn ◽  
Jeffrey G Pelton ◽  
James M Berger
Keyword(s):  
Dna Primase ◽  
Template Tracking

DNA-Polymerases α, δ and ε from T-Cell Spontaneous Lymphoblastic Leukemia of Sprague-Dawley Inbred Rat: Isolation and Characterization

1995 ◽  
Vol 60 (9) ◽  
pp. 1555-1572 ◽  
Author(s):  
Pavel Kramata ◽  
Jaroslav Černý ◽  
Gabriel Birkuš ◽  
Ivan Votruba ◽  
Berta Otová ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Lymphoblastic Leukemia ◽  
Dna Polymerases ◽  
Sedimentation Coefficient ◽  
Nuclear Antigen ◽  
Exonuclease Activity ◽  
Sprague Dawley ◽  
Dna Primase ◽  
Isolation And Characterization ◽  
Inbred Rat

Using a single isolation procedure and selective assays for the determination of enzyme activity, highly purified DNA-polymerases α, δ and ε were isolated from the lymphoma of Sprague-Dawley inbred rats. For pol α the subunit composition was 170, 70, 57 and 53 kDa with sedimentation coefficient 8.7 S for the native molecule; pol delta consists of two polypeptides (133 and 46 kDa; sedimentation coefficient 8.2 S), while pol ε is a single polypeptide (140 kDa) and its sedimentation coefficient is 7.0 S. Comparison of the interaction of individual enzymes with known inhibitors and proliferating cell nuclear antigen (PCNA) using the template-primer poly dA-oligo dT12-18, gave the following data: (i) pol α is selectively inhibited by N2-(p-butylphenyl)-2'-deoxyguanosine 5'-triphosphate (BuPdGTP) and stimulated by dimethyl sulfoxide; (ii) all the enzymes are inhibited by N-ethylmaleimide and aphidicolin; (iii) PCNA stimulates pol δ approximately 50 times while pol ε is moderately inhibited; (iv) pol α exhibits considerably higher DNA-primase activity with poly dC as template than with poly dT, and negligible 3'-5'-exonuclease activity whereas pol δ and pol ε, which do not exert any DNA-primase activity have approximately the same 3'-5'-exonuclease activity. The ability of individual polymerases to utilize poly dT-oligo dA12-18 as a template-primer at different pH values, ionic strengths and Mg2+-concentrations was also investigated. In comparison to poly dA-oligo dT12-18 template-primer, pol α has 140% of enzyme activity on poly dT-oligo dA12-18 under optimal conditions, whereas the activity of pol ε and pol δ is 4 times and 10 times lower, respectively.

Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) Subunit Genes of the Human DNA Primase to Chromosome Bands 1q44 and 6p11.1–p12

Genomics ◽  
1997 ◽  
Vol 44 (2) ◽  
pp. 251-252
Author(s):  
Akiko Shiratori ◽  
Katsuzumi Okumura ◽  
Masahiro Nogami ◽  
Hiroshi Taguchi ◽  
Tokiro Onozaki ◽  
...  
Keyword(s):  
Dna Primase ◽  
Human Dna ◽  
Chromosome Bands

scholarly journals A DNA primase from yeast. Purification and partial characterization.

1985 ◽  
Vol 260 (8) ◽  
pp. 4995-5002
Author(s):  
S M Jazwinski ◽  
G M Edelman
Keyword(s):  
Partial Characterization ◽  
Dna Primase

scholarly journals Tight association of DNA primase with a subspecies of mouse DNA polymerase alpha.

1983 ◽  
Vol 258 (11) ◽  
pp. 6698-6700 ◽  
Author(s):  
T Yagura ◽  
S Tanaka ◽  
T Kozu ◽  
T Seno ◽  
D Korn
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase Alpha ◽  
Dna Primase ◽  
Tight Association

scholarly journals DNA primase. Processivity and the primase to polymerase alpha activity switch.

1990 ◽  
Vol 265 (27) ◽  
pp. 16158-16165 ◽  
Author(s):  
R D Kuchta ◽  
B Reid ◽  
L M Chang
Keyword(s):  
Alpha Activity ◽  
Dna Primase

scholarly journals DNA primase-DNA polymerase alpha from simian cells. Modulation of RNA primer synthesis by ribonucleoside triphosphates.

1985 ◽  
Vol 260 (10) ◽  
pp. 6254-6263
Author(s):  
M Yamaguchi ◽  
E A Hendrickson ◽  
M L DePamphilis
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase Alpha ◽  
Dna Primase
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