RIT1 suppresses esophageal squamous cell carcinoma growth and metastasis and predicts good prognosis

Abstract Background Tumor associated macrophages and tumor infiltrating lymphocytes contribute significantly to the development of immunosuppressive properties of tumor. In this study we performed immunohistochemical analysis of immune cells of esophageal tumors stroma. Methods Paraffin‑embedded tissue specimens from 48 esophageal squamous cell carcinoma patients were retrospectively collected for immunohistochemical analysis of stromal cells. For staining of macrophages, CD68, CD163, CD206, PU.1 and iNOS were used. For T-cells detection CD8, CD3, FOXP3 were used. As well we performed staining for PD-L1 that can be expressed on tumor associated macrophagesand tumor cells. Clinicopathological and survival data were collected and analyzed using the χ2 and Fisher exact tests, Kaplan–Meier curves, and the log‑rank test. The correlation analysis was performed with Spearman correlation coefficient. Results The level of CD206 expression was associated with histological grade (p = 0.034), FOXP3 expression was associated with sex and age (p = 0.041, p = 0.003 respectively) and iNOS expression was associated with the disease stage (p = 0.044). In addition, FOXP3 and CD163 appeared to be markers of good prognosis (HR = 0.5407, p = 0.0462; HR = 0.4447, p = 0.0456 respectively). Significant association between PU.1 + and CD68 + macrophages (r = 0.752; P = 0.000) and between PU.1 + and CD163 + macrophages (r = 0.585; P = 0.000) was established, positive association between PU.1 and CD206 expression was also observed (r = 0.424; P = 0.001). Conclusions Large amounts of CD163 + macrophages and FOXP3+ Т-cells appear to be markers of good prognosis of esophageal squamous cell carcinoma. The number of PU.1 + macrophages strongly correlate with the number of CD68 + macrophages therefore usage of PU.1 as a potential macrophage marker can be recommended esophageal tumors.

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506 TO STUDY THE CLINICAL IMPACT AND PROGNOSTIC VALUE OF TUMOR INFILTRATING LYMPHOCYTES IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA

Diseases of the Esophagus ◽

10.1093/dote/doaa087.132 ◽

2020 ◽

Vol 33 (Supplement_1) ◽

Author(s):

N Uma Baskaran ◽

A M ◽

S Rajendiran

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Tumor Infiltrating Lymphocytes ◽

Good Prognosis ◽

Tumour Tissue ◽

Stromal Compartment ◽

Tumour Infiltrating Lymphocytes ◽

Infiltrating Lymphocytes

Abstract Esophageal cancer is the sixth most common cause of death due to cancer worldwide, of which Esophageal Squamous cell carcinoma (ESCC) is the most prevalent type. The presence of Tumour Infiltrating Lymphocytes (TILs) in a tumour indicates a good prognosis of the cancer following immunotherapy. Numeral immunological inhibitors have been developed to treat a variety of cancers, however the role of TILs in ESCC has not been substantiated with proper evidence. Methods The study enrolled 27 patients of ESCC between the years 2014–2019.The evaluation was based on the criteria laid down by the International TILs Working Group 2014 (recorded in percentage based on the area of stromal compartment invaded by the mononuclear inflammatory cells). Scoring was done in 400x field as follows: Score 0- No infiltrating lymphocytes. Score 1- Mild increase in infiltrating lymphocytes in the tumour nest or stroma. Score 2- Increase in infiltrating lymphocytes interwoven with the tumour tissue. Score 3- Prominent infiltrating lymphocytes incorporated in the tumour tissue. Results A total of 27 cases were studied, of which 17 were males (63%) and 10 were females (37%). The commonest age group was 60–70 years (12 cases- 44.4%). 22 cases had tumours sized between 3-7 cm (81.4%). pT1–1 case (0.03%). pT2–10 cases (37.03%). pT3–16 cases (59.25%). TILs were further scored based on the standard scoring system, and the results were as follows: Score 0 in 3 cases (11.11%). Score 1 in 14 cases (51.8%). Score 2 in 4 cases (14.8%). Score 3 in 6 cases (22.22%). Conclusion In conclusion, the commonest score of 1 was seen in 51.8% of the cases and that of score 3 was seen in 22.22% of the cases. Tumour Infiltrating Lymphocytes can hence serve as a predictor for good prognosis of Esophageal Squamous Cell Carcinoma following surgery or radiotherapy/chemotherapy.

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CLINICAL IMPACT AND PROGNOSTIC VALUE OF TUMOR INFILTRATING LYMPHOCYTES IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA

10.36106/gjra/8901058 ◽

2020 ◽

pp. 1-4

Author(s):

Nikitha Uma Baskaran ◽

Arthi M ◽

Swaminathan Rajendiran

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Tumor Infiltrating Lymphocytes ◽

Good Prognosis ◽

Age Group ◽

Tumour Infiltrating Lymphocytes ◽

Infiltrating Lymphocytes

Background: Esophageal Squamous cell carcinoma is the most prevalent type of Esophageal cancer in the world. The presence of Tumour infiltrating lymphocytes indicates a good prognosis of the cancer and this may suggest a good response to immunotherapy. Numerous immunological inhibitors have been developed to treat a variety of cancers; however, the role of Tumour Infiltrating Lymphocytes in Esophageal Squamous Cell Carcinoma has not been substantiated with proper evidence. Methodology: The study enrolled 27 patients of Esophageal Squamous Cell Carcinoma between the years 2014-2019. The evaluation was based on the criteria laid down by the International Tumour Infiltrating Lymphocytes Working Group 2014 and scoring of the same was done in 400x field and were reported. Results: A total of 27 cases were studied, of which 17 were males and 10 were females. The commonest age group was 60-70 years. 22 cases had tumours sized between 3-7cm. Tumour grading of pT1 was seen in 1 case, grading of pT2 in 10 cases and pT3 in 16 cases. Tumour Infiltrating Lymphocytes were scored based on the standard scoring system, and the results showed that 3 cases were of Score 0, 14 cases of Score 1, 4 cases of Score 2, and 6 cases of Score 3. Conclusion: The commonest score of 1 was seen in 51.8% of the cases and that of score 3 was seen in 22.22% of the cases. Tumour Infiltrating Lymphocytes can hence serve as a predictor for good prognosis of Esophageal Squamous Cell Carcinoma following surgery or radiotherapy/chemotherapy.

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