Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain
AbstractAlthough prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.
2002 ◽
Vol 44
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pp. 449-460
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1999 ◽
Vol 96
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pp. 1580-1584
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1985 ◽
Vol 260
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pp. 2301-2306
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1982 ◽
Vol 257
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pp. 6102-6105
2021 ◽
Vol 548
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pp. 39-46
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