Transcriptome analysis in Hevea brasiliensis latex revealed changes in hormone signalling pathways during ethephon stimulation and consequent Tapping Panel Dryness

Ethylene is a hormone known for its involvement in the process of latex harvesting in Hevea brasiliensis. It facilitates latex flow by activation of endogenous metabolism in the anastomosed latex cells called laticifers. In regard to its ambivalent role, ethylene is both favourable to the latex production and unfavourable, to a certain level, to the apparition of a physiological disease termed as tapping panel dryness (TPD). Comprehensive researches have been carried out to reveal the molecular actors in ethylene biosynthesis and signalling pathways in Hevea brasiliensis. One of the most important superfamily implicated as the last transcription factor known in plant ethylene signalling is the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF). Currently, 114 unique sequences related to the Hevea AP2/ERF gene superfamily have been identified and characterized. Specific characterizations under the condition of harvesting stress and the occurrence of TPD have identified 36 gene expression markers (GEMs). Eighteen of these GEMs were predicted as ortholog with 19 Arabidopsis AP2/ERF genes. The characterization was mainly focused on transcriptional regulation, whilst potential post-transcriptional and post-translational regulations of HbAP2/ERF genes were formerly predicted. Three HbERF groups (HbERF-VII, HbERF-VIII and HbERF-IX) were hypothesized to have an important role in Hevea tolerance during latex production as they highly accumulated in laticifers and in response to multiple abiotic stresses. Further functional analysis of several key genes is suggested in order to fully understand the regulation of HbAP2/ERFs. Finally, the molecular markers for future Hevea breeding could be possibly developed from this superfamily.

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The Hevea brasiliensis AP2/ERF superfamily: from ethylene signalling to latex harvesting and physiological disease response

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v84i1.201 ◽

2016 ◽

Vol 84 (1) ◽

Cited By ~ 1

Author(s):

Riza Arief PUTRANTO ◽

Pascal MONTORO

Keyword(s):

Hevea Brasiliensis ◽

Ethylene Biosynthesis ◽

Signalling Pathways ◽

Ethylene Response Factor ◽

Disease Response ◽

Ethylene Response ◽

Key Genes ◽

Tapping Panel Dryness ◽

Ethylene Signalling ◽

Latex Production

Ethylene is a hormone known for its involvement in the process of latex harvesting in Hevea brasiliensis. It facilitates latex flow by activation of endogenous metabolism in the anastomosed latex cells called laticifers. In regard to its ambivalent role, ethylene is both favourable to the latex production and unfavourable, to a certain level, to the apparition of a physiological disease termed as tapping panel dryness (TPD). Comprehensive researches have been carried out to reveal the molecular actors in ethylene biosynthesis and signalling pathways in Hevea brasiliensis. One of the most important superfamily implicated as the last transcription factor known in plant ethylene signalling is the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF). Currently, 114 unique sequences related to the Hevea AP2/ERF gene superfamily have been identified and characterized. Specific characterizations under the condition of harvesting stress and the occurrence of TPD have identified 36 gene expression markers (GEMs). Eighteen of these GEMs were predicted as ortholog with 19 Arabidopsis AP2/ERF genes. The characterization was mainly focused on transcriptional regulation, whilst potential post-transcriptional and post-translational regulations of HbAP2/ERF genes were formerly predicted. Three HbERF groups (HbERF-VII, HbERF-VIII and HbERF-IX) were hypothesized to have an important role in Hevea tolerance during latex production as they highly accumulated in laticifers and in response to multiple abiotic stresses. Further functional analysis of several key genes is suggested in order to fully understand the regulation of HbAP2/ERFs. Finally, the molecular markers for future Hevea breeding could be possibly developed from this superfamily.

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Latex-specific transcriptome analysis reveals mechanisms for latex metabolism and natural rubber biosynthesis in laticifers of Hevea brasiliensis

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113835 ◽

2021 ◽

Vol 171 ◽

pp. 113835

Author(s):

Xiangyu Long ◽

Yongjun Fang ◽

Yunxia Qin ◽

Jianghua Yang ◽

Xiaohu Xiao

Keyword(s):

Natural Rubber ◽

Transcriptome Analysis ◽

Hevea Brasiliensis ◽

Rubber Biosynthesis ◽

Natural Rubber Biosynthesis

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Latex production with depolymerizing compounds of actin cytoskeleton in rubber trees

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2008000200018 ◽

2008 ◽

Vol 43 (2) ◽

pp. 275-279 ◽

Cited By ~ 1

Author(s):

Gao Zheng-Quan ◽

Meng Chun-Xiao ◽

Ye Nai-Hao

Keyword(s):

Actin Cytoskeleton ◽

Potassium Iodide ◽

Hevea Brasiliensis ◽

Total Solid ◽

Inorganic Phosphorus ◽

Latrunculin A ◽

Tapping Panel Dryness ◽

Latex Production

The objective of this work was to assess stimulated latex flow from rubber trees (Hevea brasiliensis) with saturated macrolide (latrunculin A), 1, 5, and 10% potassium iodide in 2% methylcellulose compared with 0.3% ethylene in 2% methylcellulose (check) and 2% methylcellulose (blank). Latex output and contents of pure rubber, total solid, sucrose, inorganic phosphorus, thiol, and Mg2+ were measured. The treatments containing 1% KI or saturated macrolide increased latex yields compared to the blank with 2% methylcellulose alone. The 1% KI or saturated macrolide treatments were equal to that of 0.3% ethylene check treatment. However, 5 and 10% KI were harmful to bark of rubber trees, even caused prolonged tapping panel dryness.

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