transcriptome analysis
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2022 ◽  
Vol 295 ◽  
pp. 110824
Mengqian Lu ◽  
Yifan Li ◽  
Huiyan Jia ◽  
Zuguo Xi ◽  
Qijuan Gao ◽  

Aquaculture ◽  
2022 ◽  
Vol 549 ◽  
pp. 737792
Zhi Hu ◽  
Jie Feng ◽  
Hao Song ◽  
Cong Zhou ◽  
Zheng-Lin Yu ◽  

2022 ◽  
Vol 293 ◽  
pp. 110714
Shuang Jiang ◽  
Jun Luo ◽  
Xiaoqing Wang ◽  
Haishan An ◽  
Jiaying Zhang ◽  

2022 ◽  
Vol 176 ◽  
pp. 114353
Pooja Thapa ◽  
Bhuvnesh Sareen ◽  
Mohit Kumar Swarnkar ◽  
Anil Sood ◽  
Amita Bhattacharya

BMC Genomics ◽  
2022 ◽  
Vol 23 (1) ◽  
Yiqin Wang ◽  
Zhifen Yang ◽  
Luxi Shi ◽  
Rui Yang ◽  
Hao Guo ◽  

Abstract Background Drought stress severely restricts edible fungus production. The genus Auricularia has a rare drought tolerance, a rehydration capability, and is nutrient rich. Results The key genes and metabolic pathways involved in drought-stress and rehydration were investigated using a transcriptome analysis to clarify the relevant molecular mechanisms. In total, 173.93 Mb clean reads, 26.09 Gb of data bulk, and 52,954 unigenes were obtained. Under drought-stress and rehydration conditions, 14,235 and 8539 differentially expressed genes, respectively, were detected. ‘Tyrosine metabolic’, ‘caffeine metabolism’, ‘ribosome’, ‘phagosome’, and ‘proline and arginine metabolism’, as well as ‘peroxisome’ and ‘mitogen-activated protein kinase signaling’ pathways, had major roles in A. fibrillifera responses to drought stress. ‘Tyrosine’ and ‘caffeine metabolism’ might reveal unknown mechanisms for the antioxidation of A. fibrillifera under drought-stress conditions. During the rehydration process, ‘diterpenoid biosynthesis’, ‘butanoate metabolism’, ‘C5-branched dibasic acid’, and ‘aflatoxin biosynthesis’ pathways were significantly enriched. Gibberellins and γ-aminobutyric acid were important in the recovery of A. fibrillifera growth after rehydration. Many genes related to antibiotics, vitamins, and other health-related ingredients were found in A. fibrillifera. Conclusion These findings suggested that the candidate genes and metabolites involved in crucial biological pathways might regulate the drought tolerance or rehydration of Auricularia, shedding light on the corresponding mechanisms and providing new potential targets for the breeding and cultivation of drought-tolerant fungi.

2022 ◽  
Vol 12 ◽  
Qingping Ma ◽  
Laichao Song ◽  
Zhanhai Niu ◽  
Jingshan Li ◽  
Yu Wang ◽  

“Huangjinya” is a light-sensitive albino variety and is widely cultivated in China. It has been proved that red light could promote the vegetable growth of plants. However, the mechanism of “Huangjinya” in response to a red light is unclear. This study used high-throughput sequencing technology to analyze the transcriptome of tender shoots of “Huangjinya” under the white and red light supplement conditions. At the same time, liquid chromatography tandem mass spectrometry (LC-MS) was used to analyze metabolite changes under different light conditions. Transcriptome analysis revealed that a total of 174 differentially expressed genes (DEGs) were identified after the red light supplement. Kyoto encyclopedia of genes and genomes (KEGG) classification indicated that amino acid metabolism enriched the most DEGs. In addition, two phenylpropanoid metabolism-related genes and five glutathione S-transferase genes (CsGSTs) were found to be expressed differently. Metabolome analysis revealed that 193 differential metabolites were obtained. Being the same as transcriptome analysis, most differential metabolites were enriched in amino acids, sweet and umami tasting amino acids were increased, and bitter-tasting amino acids were decreased after the red light supplement. In summary, red light supplementary treatment may be propitious to the quality of “Huangjinya” due to its regulatory effect on amino acid metabolism. Also, CsGSTs involved phenylpropanoid metabolism contributed to tea quality changes in “Huangjinya.”

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