scholarly journals Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Kar Wey Yong ◽  
Belinda Pingguan-Murphy ◽  
Feng Xu ◽  
Wan Abu Bakar Wan Abas ◽  
Jane Ru Choi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Functional Characterization ◽  
Adipose Derived Stem Cells

Metabolic and Functional Characterization of Human Adipose-Derived Stem Cells in Tissue Engineering

2008 ◽  
Vol 122 (3) ◽  
pp. 725-738 ◽  
Author(s):  
Blaine T. Mischen ◽  
Keith E. Follmar ◽  
Kurtis E. Moyer ◽  
Ben Buehrer ◽  
Kevin C. Olbrich ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Functional Characterization ◽  
Adipose Derived Stem Cells

scholarly journals Effects of age on biological and functional characterization of adipose-derived stem cells from patients with end-stage liver disease

2017 ◽  
Vol 16 (3) ◽  
pp. 3510-3518 ◽  
Author(s):  
Yanyu Jin ◽  
Lei Yang ◽  
Yanyan Zhang ◽  
Wei Gao ◽  
Zhi Yao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Liver Disease ◽  
Functional Characterization ◽  
Adipose Derived Stem Cells ◽  
End Stage Liver Disease ◽  
End Stage

scholarly journals In vitro enhancement and functional characterization of neurite outgrowth by undifferentiated adipose-derived stem cells

2018 ◽  
Author(s):  
Vesna Bucan ◽  
Malte Fliess ◽  
Reinhild Schnabel ◽  
Claas‑Tido Peck ◽  
Desiree Vaslaitis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neurite Outgrowth ◽  
Functional Characterization ◽  
Adipose Derived Stem Cells

scholarly journals Long term culture and differentiation of endothelial progenitor like cells from rat adipose derived stem cells

2017 ◽  
Vol 70 (1) ◽  
pp. 397-413 ◽  
Author(s):  
Monire Amerion ◽  
Mojtaba Rezazadeh Valojerdi ◽  
Saeid Abroun ◽  
Mehdi Totonchi
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Endothelial Progenitor ◽  
Long Term Culture

Characterization of Adipose-Derived Stem Cells: An Update

2010 ◽  
Vol 5 (2) ◽  
pp. 95-102 ◽  
Author(s):  
Alexander M. Bailey ◽  
Sahil Kapur ◽  
Adam J. Katz
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells

Functional Characterization of Des-IGF-1 Action at Excitatory Synapses in the CA1 Region of Rat Hippocampus

2005 ◽  
Vol 94 (1) ◽  
pp. 247-254 ◽  
Author(s):  
Melinda M. Ramsey ◽  
Michelle M. Adams ◽  
Olusegun J. Ariwodola ◽  
William E. Sonntag ◽  
Jeff L. Weiner
Keyword(s):  
Synaptic Transmission ◽  
Hippocampal Slices ◽  
Functional Characterization ◽  
Excitatory Postsynaptic Potential ◽  
Excitatory Synapses ◽  
Aged Rats ◽  
Cognitive Improvement ◽  
Ca1 Region

Insulin-like growth factor-1 (IGF-1) and growth hormone play a major role in the growth and development of tissues throughout the mammalian body. Plasma IGF-1 concentrations peak during puberty and decline with age. We have determined that chronic treatments to restore plasma IGF-1 concentrations to adult levels attenuate spatial learning deficits in aged rats, but little is known of the acute actions of IGF-1 in the brain. To this end, we utilized hippocampal slices from young Sprague-Dawley rats to characterize the acute effects of des-IGF-1 on excitatory synaptic transmission in the CA1 region. We observed a 40% increase in field excitatory postsynaptic potential (fEPSP) slope with application of des-IGF-1 (40 ng/ml) and used whole cell patch-clamp recordings to determine that this enhancement was due to a postsynaptic mechanism involving α-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate (AMPA) but not N-methyl-d-aspartate receptors. Furthermore, the enhancement was completely blocked by the broad-spectrum tyrosine kinase inhibitor, genistein (220 μM), and significantly reduced by the PI3K blockers wortmannin (1 μM) and 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (10 μM), suggesting that the effect was predominantly dependent on PI3K activation. This characterization of the acute actions of des-IGF-1 at hippocampal excitatory synapses may provide insight into the mechanism by which long-term increases in plasma IGF-1 impart cognitive benefits in aged rats. Increases in AMPA receptor-mediated synaptic transmission may contribute directly to cognitive improvement or initiate long-term changes in synthesis of proteins such as brain-derived neurotrophic factor that are important to learning and memory.

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