CHAPTER 14. Oxygen Reduction and Proton Translocation by Respiratory Cytochrome c Oxidase

Author(s):  
Mårten Wikström ◽  
Vivek Sharma
2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S227
Author(s):  
Tatsuhito Nishiguchi ◽  
Masahide Hikita ◽  
Kyoko Shinzawa-Itoh ◽  
Shinya Yoshikawa ◽  
Satoru Nakashima ◽  
...  

1979 ◽  
Vol 182 (1) ◽  
pp. 149-156 ◽  
Author(s):  
R P Casey ◽  
J B Chappell ◽  
A Azzi

We have investigated ferrocytochrome c-induced proton ejection from reconstituted cytochrome c oxidase-containing vesicles using careful control of the number of enzyme turnovers. Ferrocytochrome c caused the appearance of protons at the vesicle exterior, and this could be abolished by using a protonophore. In addition, its decay was dependent on the permeability of the vesicle membranes to protons and the number of turnovers of the oxidase. These observations indicate that the ejection of protons was the result of genuine translocation. The possibility of this translocation occurring via a Mitchellian loop as a result of the presence of a reduced hydrogen carrier contaminating the enzyme was considered and excluded. Proton-translocating activity in this reconstituted system depended critically on the ratio of enzyme to lipid used in the reconstitution process and we propose a rationale to account for this. We conclude that our data provide strong support for the proposal that cytochrome c oxidase acts as a proton pump and that approx. 0.9 H+ is excluded per ferrocytochrome c molecule oxidized.


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