Silver metallation of hen egg white lysozyme: X-ray crystal structure and NMR studies

A crystal of hen egg-white lysozyme was analyzed by means of energy-dispersive X-ray Laue diffraction with white synchrotron radiation at 2.7 Å resolution using a pnCCD detector. From Laue spots measured in a single exposure of the arbitrarily oriented crystal, the lattice constants of the tetragonal unit cell could be extracted with an accuracy of about 2.5%. Scanning across the sample surface, Laue images with split reflections were recorded at various positions. The corresponding diffraction patterns were generated by two crystalline domains with a tilt of about 1° relative to each other. The obtained results demonstrate the potential of the pnCCD for fast X-ray screening of crystals of macromolecules or proteins prior to conventional X-ray structure analysis. The described experiment can be automatized to quantitatively characterize imperfect single crystals or polycrystals.

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Preliminary X-ray data for two new forms of hen egg-white lysozyme

Acta Crystallographica ◽

10.1107/s0365110x67003378 ◽

1967 ◽

Vol 23 (4) ◽

pp. 666-666 ◽

Cited By ~ 7

Author(s):

D. J. Haas

Keyword(s):

Egg White ◽

Hen Egg White Lysozyme ◽

X Ray ◽

Egg White Lysozyme ◽

Hen Egg White ◽

Hen Egg

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Crystal distortion of monoclinic hen egg-white lysozyme crystals using X-ray digital topography

Journal of Crystal Growth ◽

10.1016/j.jcrysgro.2014.01.036 ◽

2014 ◽

Vol 401 ◽

pp. 238-241

Author(s):

Kei Wako ◽

Daiki Fujii ◽

Shiro Tsukashima ◽

Takeharu Kishi ◽

Masaru Tachibana ◽

...

Keyword(s):

Egg White ◽

Hen Egg White Lysozyme ◽

X Ray ◽

Egg White Lysozyme ◽

Hen Egg White ◽

Lysozyme Crystals ◽

Hen Egg

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Structure of amyloid fibrils of hen egg white lysozyme studied by microbeam X-ray diffraction

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2009.04.007 ◽

2009 ◽

Vol 45 (1) ◽

pp. 86-90 ◽

Cited By ~ 18

Author(s):

Naoto Yagi ◽

Noboru Ohta ◽

Tatsuhito Matsuo

Keyword(s):

Amyloid Fibrils ◽

Egg White ◽

Hen Egg White Lysozyme ◽

X Ray Diffraction ◽

X Ray ◽

Egg White Lysozyme ◽

Hen Egg White ◽

Hen Egg

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Synchrotron Radiation Small-Angle X-Ray Scattering Study of Structural Transition and Gelation of Hen Egg-White Lysozyme Depending on pH and Ionic Strength under Isothermal Heating.

KOBUNSHI RONBUNSHU ◽

10.1295/koron.55.653 ◽

1998 ◽

Vol 55 (10) ◽

pp. 653-660

Author(s):

Shigeki ARAI ◽

Mitsuhiro HIRAI

Keyword(s):

Structural Transition ◽

Egg White ◽

Hen Egg White Lysozyme ◽

Isothermal Heating ◽

X Ray ◽

Egg White Lysozyme ◽

X Ray Scattering ◽

Scattering Study ◽

Hen Egg White ◽

Hen Egg

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Hydrogen/deuterium exchange behavior in tetragonal hen egg-white lysozyme crystals affected by solution state

Journal of Applied Crystallography ◽

10.1107/s1600576720005488 ◽

2020 ◽

Vol 53 (3) ◽

pp. 837-840

Author(s):

Akiko Kita ◽

Yukio Morimoto

Keyword(s):

Egg White ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Hen Egg White Lysozyme ◽

Nmr Studies ◽

Egg White Lysozyme ◽

Hydrogen Deuterium ◽

Hen Egg White ◽

Lysozyme Crystals ◽

Hen Egg

Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period.

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