Tethering of spherical DOTAP liposome gold nanoparticles on cysteamine monolayer for sensitive label free electrochemical detection of DNA and transfection

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2467-2475 ◽  
Author(s):  
Mohanlal Bhuvana ◽  
Venkataraman Dharuman

Cysteamine monolayer supported spherical DOTAP–AuNP on gold electrode is developed for DNA label free sensing and transfection.

RSC Advances ◽  
2015 ◽  
Vol 5 (70) ◽  
pp. 56583-56589 ◽  
Author(s):  
Yulan Wang ◽  
Dan Wu ◽  
Yong Zhang ◽  
Xiang Ren ◽  
Yaoguang Wang ◽  
...  

In this work, a novel and ultrasensitive label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP).


2013 ◽  
Vol 310 ◽  
pp. 177-182
Author(s):  
Song Bai Zhang ◽  
Bing Jun Zhang ◽  
Qian Liu ◽  
Xia Hu ◽  
Li Ying Zheng ◽  
...  

A label-free electrochemical biosensing strategy based on gold nanoparticle involved layer-by-layer self assembly for the detection of protein is proposed using platelet derived growth factor-BB dimer (PDGF-BB) as the model analyte. Utilizing the strong sulfur-Au affinity, ethanthiol and capture probe modified gold nanoparticles are self-assembled onto the surface of gold electrode successively. The aptamer probe for target protein hybridizes with the capture probe and the biosensor is fabricated. By measuring ac current voltammetry, the target protein can be sensitively detected in a linear dynamic range from 1-1000 ng/mL with a low detection limit of 0.5 ng/mL. Making use of self-assembled gold nanoparticles layer, a large amount of capture probes can be modified onto the gold electrode, supporting the high sensitivity of the proposed strategy. In addition, good reproducibility, high selectivity and stability are achieved. In particular, the biosensor can be easily regenerated by melting in hot water, making it reusable.


2018 ◽  
Vol 120 ◽  
pp. 122-128 ◽  
Author(s):  
Shokoufeh Hassani ◽  
Milad Rezaei Akmal ◽  
Armin Salek-Maghsoudi ◽  
Soheila Rahmani ◽  
Mohammad Reza Ganjali ◽  
...  

2003 ◽  
Vol 5 (10) ◽  
pp. 887-891 ◽  
Author(s):  
Kagan Kerman ◽  
Yasutaka Morita ◽  
Yuzuru Takamura ◽  
Eiichi Tamiya

2011 ◽  
Vol 173 (3-4) ◽  
pp. 299-305 ◽  
Author(s):  
Ping Tong ◽  
Shurong Tang ◽  
Yu He ◽  
Yanhong Shao ◽  
Lan Zhang ◽  
...  

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