Mass spectrometric investigation of the role of the linking polypeptide chain in DNA polymerase I

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2432-2439 ◽  
Author(s):  
Taeho Yeom ◽  
Jungyoon Lee ◽  
Seonghyun Lee ◽  
Sunah Kang ◽  
Kyung Rok Kim ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Polypeptide Chain ◽  
Mass Spectrometric ◽  
Spectrometric Investigation ◽  
Mass Spectrometric Investigation ◽  
Dna Polymerase I ◽  
Maldi Tof ◽  
Pol I ◽  
Polymerase I

MALDI-TOF analysis elucidates the functions of two domains in pol I.

scholarly journals DNA Polymerase I Is Not Required for Replication of Linear Chromosomes in Streptomyces

2007 ◽  
Vol 190 (2) ◽  
pp. 755-758 ◽  
Author(s):  
Tzu-Wen Huang ◽  
Carton W. Chen
Keyword(s):  
Dna Polymerase ◽  
Double Mutant ◽  
Slow Growth ◽  
Dna Polymerase I ◽  
Uv Sensitivity ◽  
Pol I ◽  
Linear Chromosomes ◽  
Polymerase I

ABSTRACT Both polA (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the ΔpolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes.

THE INFLUENCE OF THE PO1A1 MUTATION UPON RECOMBINATION IN ESCHERICHIA COLI K12

1979 ◽  
Vol 21 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Barry W. Glickman ◽  
Tineke Rutgers
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Genetic Recombination ◽  
Dna Polymerase I ◽  
Multiple Gene ◽  
E Coli ◽  
Recombinational Process ◽  
K 12 ◽  
Polymerase I

Genetic recombination in Escherichia coli is a highly regulated process involving multiple gene products. We have investigated the role of DNA polymerase I in this process by studying the effect of the po1A1 mutation upon DNA transfer and conjugation in otherwise isogenic suppressor-free strains of E. coli K-12. It was found that the po1A1 mutation greatly reduces recombination in Hfr crosses (a factor of 20 in Po1+ × Po1A1 crosses and more than a factor of 100 in Po1A1 × Po1A1 crosses). However, since the po1A1 mutation reduces the strains capacity to act as a recipient for an F-prime and the analysis of recombination transfer gradients revealed no differences between Po1+ and Po1− strains, it is concluded that DNA polymerase I probably affects the transfer and/or stability of donor DNA rather than the recombinational process itself.

scholarly journals Role of High-Fidelity Escherichia coli DNA Polymerase I in Replication Bypass of a Deoxyadenosine DNA-Peptide Cross-Link

2011 ◽  
Vol 193 (15) ◽  
pp. 3815-3821 ◽  
Author(s):  
K. Yamanaka ◽  
I. G. Minko ◽  
S. E. Finkel ◽  
M. F. Goodman ◽  
R. S. Lloyd
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
High Fidelity ◽  
Cross Link ◽  
Dna Polymerase I ◽  
Polymerase I

scholarly journals DNA polymerase I (Klenow fragment): Role of the structure and length of a template in enzyme recognition

FEBS Letters ◽  
1989 ◽  
Vol 248 (1-2) ◽  
pp. 97-100 ◽  
Author(s):  
T.I. Kolocheva ◽  
G.A. Nevinsky ◽  
V.A. Volchkova ◽  
A.S. Levina ◽  
V.V. Khomov ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Klenow Fragment ◽  
Dna Polymerase I ◽  
Polymerase I ◽  
Enzyme Recognition

Biochemical characterization of human DNA polymerase i provides clues to its biological function

2001 ◽  
Vol 29 (2) ◽  
pp. 183-187 ◽  
Author(s):  
A. Tissier ◽  
E. G. Frank ◽  
J. P. McDonald ◽  
A. Vaisman ◽  
A. R. Fernàndez deHenestrosa Henestrosa ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Biochemical Characterization ◽  
Short Review ◽  
Biochemical Properties ◽  
Dna Polymerase I ◽  
Polymerase I

The human RAD30B gene has recently been shown to encode a novel DNA polymerase, DNA polymerase i (poli). The role of poli within the cell is presently unknown, and the only clues to its cellular function come from its biochemical characterization in vitro. The aim of this short review is, therefore, to summarize the known enzymic activities of poli and to speculate as to how these biochemical properties might relate to its in vivo function.

Role of the 5´ → 3´ exonuclease and Klenow fragment of Escherichia coli DNA polymerase I in base mismatch repair

2007 ◽  
Vol 278 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Masaru Imai ◽  
Yu-ichiro Tago ◽  
Makoto Ihara ◽  
Masakado Kawata ◽  
Kazuo Yamamoto
Keyword(s):  
Escherichia Coli ◽  
Mismatch Repair ◽  
Dna Polymerase ◽  
Klenow Fragment ◽  
Dna Polymerase I ◽  
Base Mismatch ◽  
Polymerase I
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