Fluorescence axial nanotomography with plasmonics

2015 ◽  
Vol 178 ◽  
pp. 371-381 ◽  
Author(s):  
Nicholas I. Cade ◽  
Gilbert O. Fruhwirth ◽  
Alexey V. Krasavin ◽  
Tony Ng ◽  
David Richards

We present a novel imaging technique with super-resolution axial sensitivity, exploiting the changes in fluorescence lifetime above a plasmonic substrate. Using conventional confocal fluorescence lifetime imaging, we show that it is possible to deliver down to 6 nm axial position sensitivity of fluorophores in whole biological cell imaging. We employ this technique to map the topography of the cellular membrane, and demonstrate its application in an investigation of receptor-mediated endocytosis in carcinoma cells.

1994 ◽  
Vol 4 (4) ◽  
pp. 291-294 ◽  
Author(s):  
Renata Sanders ◽  
Hans C. Gerritsen ◽  
Arie Draaijer ◽  
Piet M. Houpt ◽  
Yehudi K. Levine

Author(s):  
Zhaotai Gu ◽  
Cuifang Kuang ◽  
Shuai Li ◽  
Yi Xue ◽  
Zhenrong Zheng ◽  
...  

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