scholarly journals Fluorophore ATCUN complexes: combining agent and probe for oxidative DNA cleavage

2015 ◽  
Vol 51 (62) ◽  
pp. 12395-12398 ◽  
Author(s):  
C. Wende ◽  
N. Kulak
Keyword(s):  
Dna Cleavage ◽  
Metal Ion ◽  
Fluorescent Reporter ◽  
Dna Cleaving ◽  
Oxidative Dna Cleavage

A Cu(ii)-based peptidic DNA cleaving agent equipped with a Cu(ii)-sensing fluorescent reporter allows monitoring the fate of the nucleolytic metal ion.

Oxidative DNA cleavage by copper ternary complexes of 1,10-phenanthroline and ethylenediamine-sulfonamide derivatives

Polyhedron ◽  
2009 ◽  
Vol 28 (13) ◽  
pp. 2537-2544 ◽  
Author(s):  
Andreea Bodoki ◽  
Adriana Hangan ◽  
Luminita Oprean ◽  
Gloria Alzuet ◽  
Alfonso Castiñeiras ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Ternary Complexes ◽  
Oxidative Dna Cleavage

On the Divalent Metal Ion Dependence of DNA Cleavage by Restriction Endonucleases of the EcoRI Family

2009 ◽  
Vol 393 (1) ◽  
pp. 140-160 ◽  
Author(s):  
Vera Pingoud ◽  
Wolfgang Wende ◽  
Peter Friedhoff ◽  
Monika Reuter ◽  
Jürgen Alves ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Metal Ion ◽  
Divalent Metal ◽  
Restriction Endonucleases ◽  
Divalent Metal Ion

scholarly journals Analysis of the HindIII-catalyzed reaction by time-resolved crystallography

2015 ◽  
Vol 71 (2) ◽  
pp. 256-265 ◽  
Author(s):  
Takashi Kawamura ◽  
Tomoki Kobayashi ◽  
Nobuhisa Watanabe
Keyword(s):  
Electron Density ◽  
Active Site ◽  
Dna Cleavage ◽  
Metal Ion ◽  
Manganese Ions ◽  
Metal Ion Binding ◽  
Time Resolved ◽  
Manganese Ion ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites

In order to investigate the mechanism of the reaction catalyzed by HindIII, structures of HindIII–DNA complexes with varying durations of soaking time in cryoprotectant buffer containing manganese ions were determined by the freeze-trap method. In the crystal structures of the complexes obtained after soaking for a longer duration, two manganese ions, indicated by relatively higher electron density, are clearly observed at the two metal ion-binding sites in the active site of HindIII. The increase in the electron density of the two metal-ion peaks followed distinct pathways with increasing soaking times, suggesting variation in the binding rate constant for the two metal sites. DNA cleavage is observed when the second manganese ion appears, suggesting that HindIII uses the two-metal-ion mechanism, or alternatively that its reactivity is enhanced by the binding of the second metal ion. In addition, conformational change in a loop near the active site accompanies the catalytic reaction.

scholarly journals The Inhibition of Phenylhydroquinone-Induced Oxidative DNA Cleavage by Constituents of Moutan Cortex and Paeoniae Radix.

2000 ◽  
Vol 23 (2) ◽  
pp. 199-203 ◽  
Author(s):  
Tomoko OKUBO ◽  
Fumiko NAGAI ◽  
Takako SETO ◽  
Kanako SATOH ◽  
Keiko USHIYAMA ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Moutan Cortex ◽  
Paeoniae Radix ◽  
Oxidative Dna Cleavage

scholarly journals Human topoisomerase II  uses a two-metal-ion mechanism for DNA cleavage

2008 ◽  
Vol 36 (15) ◽  
pp. 4883-4893 ◽  
Author(s):  
J. E. Deweese ◽  
A. B. Burgin ◽  
N. Osheroff
Keyword(s):  
Dna Cleavage ◽  
Metal Ion ◽  
Topoisomerase Ii
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