DNA binding and oxidative DNA cleavage activity of (μ-oxo)diiron(iii) complexes in visible light

2009 ◽  
pp. 1024-1033 ◽  
Author(s):  
Mithun Roy ◽  
Ramkumar Santhanagopal ◽  
Akhil R. Chakravarty
Keyword(s):  
Dna Binding ◽  
Visible Light ◽  
Dna Cleavage ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Oxidative Dna Cleavage

New ternary copper(II) complexes of l-alanine and heterocyclic bases: DNA binding and oxidative DNA cleavage activity

2009 ◽  
Vol 362 (13) ◽  
pp. 4692-4698 ◽  
Author(s):  
P.R. Chetana ◽  
Ramakrishna Rao ◽  
Mithun Roy ◽  
Ashis K. Patra
Keyword(s):  
Dna Binding ◽  
Dna Cleavage ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Heterocyclic Bases ◽  
Oxidative Dna Cleavage

Enhanced Oxidative DNA Cleavage Activity of Iron Complex of Pentadentate Mono-carboxamide Ligand Having Spermine as DNA Binding Domain

2020 ◽  
Vol 49 (11) ◽  
pp. 1353-1355
Author(s):  
Akiko Nomura ◽  
Masahito Kodera ◽  
Yutaka Hitomi
Keyword(s):  
Dna Binding ◽  
Dna Cleavage ◽  
Iron Complex ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Oxidative Dna Cleavage

scholarly journals Correction to Metal-Based Netropsin Mimics Showing AT-Selective DNA Binding and DNA Cleavage Activity at Red Light

2019 ◽  
Vol 58 (14) ◽  
pp. 9514-9514
Author(s):  
Ashis K. Patra ◽  
Tuhin Bhowmick ◽  
Suryanarayanarao Ramakumar ◽  
Akhil R. Chakravarty
Keyword(s):  
Dna Binding ◽  
Dna Cleavage ◽  
Red Light ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Selective Dna Binding

Metal-Based Netropsin Mimics Showing AT-Selective DNA Binding and DNA Cleavage Activity at Red Light

2007 ◽  
Vol 46 (22) ◽  
pp. 9030-9032 ◽  
Author(s):  
Ashis K. Patra ◽  
Tuhin Bhowmick ◽  
Suryanarayanarao Ramakumar ◽  
Akhil R. Chakravarty
Keyword(s):  
Dna Binding ◽  
Dna Cleavage ◽  
Red Light ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Selective Dna Binding

scholarly journals DNA binding induces a cis-to-trans switch in Cre recombinase to enable intasome assembly

2020 ◽  
Vol 117 (40) ◽  
pp. 24849-24858
Author(s):  
Aparna Unnikrishnan ◽  
Carlos Amero ◽  
Deepak Kumar Yadav ◽  
Kye Stachowski ◽  
Devante Potter ◽  
...  
Keyword(s):  
Dna Binding ◽  
Protein Interactions ◽  
Dna Cleavage ◽  
Conformational Dynamics ◽  
Dna Recombination ◽  
Cre Recombinase ◽  
Protein Protein Interactions ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Synaptic Complexes

Mechanistic understanding of DNA recombination in the Cre-loxP system has largely been guided by crystallographic structures of tetrameric synaptic complexes. Those studies have suggested a role for protein conformational dynamics that has not been well characterized at the atomic level. We used solution nuclear magnetic resonance (NMR) spectroscopy to discover the link between intrinsic flexibility and function in Cre recombinase. Transverse relaxation-optimized spectroscopy (TROSY) NMR spectra show the N-terminal and C-terminal catalytic domains (CreNTD and CreCat) to be structurally independent. Amide 15N relaxation measurements of the CreCat domain reveal fast-timescale dynamics in most regions that exhibit conformational differences in active and inactive Cre protomers in crystallographic tetramers. However, the C-terminal helix αN, implicated in assembly of synaptic complexes and regulation of DNA cleavage activity via trans protein–protein interactions, is unexpectedly rigid in free Cre. Chemical shift perturbations and intra- and intermolecular paramagnetic relaxation enhancement (PRE) NMR data reveal an alternative autoinhibitory conformation for the αN region of free Cre, wherein it packs in cis over the protein DNA binding surface and active site. Moreover, binding to loxP DNA induces a conformational change that dislodges the C terminus, resulting in a cis-to-trans switch that is likely to enable protein–protein interactions required for assembly of recombinogenic Cre intasomes. These findings necessitate a reexamination of the mechanisms by which this widely utilized gene-editing tool selects target sites, avoids spurious DNA cleavage activity, and controls DNA recombination efficiency.

Novel iron phenanthroline-based photosensitizers for antimicrobial PDT: synthesis, DNA binding and photo-induced DNA cleavage activity

2017 ◽  
Vol 26 (6) ◽  
pp. 1160-1169 ◽  
Author(s):  
Chittanahalli N. Sudhamani ◽  
Halehatty S. Bhojya Naik ◽  
Kalligundi R. Sangeetha Gowda ◽  
Manju Giridhar ◽  
Dugganna Girija ◽  
...  
Keyword(s):  
Dna Binding ◽  
Dna Cleavage ◽  
Cleavage Activity ◽  
Dna Cleavage Activity

scholarly journals Ternary iron(ii) complex with an emissive imidazopyridine arm from Schiff base cyclizations and its oxidative DNA cleavage activity

2005 ◽  
pp. 349 ◽  
Author(s):  
Arindam Mukherjee ◽  
Shanta Dhar ◽  
Munirathinam Nethaji ◽  
Akhil R. Chakravarty
Keyword(s):  
Schiff Base ◽  
Dna Cleavage ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Oxidative Dna Cleavage

New Insights into the Visible-Light-Induced DNA Cleavage Activity of Dipyridoquinoxaline Complexes of Bivalent 3d-Metal Ions

2007 ◽  
Vol 46 (26) ◽  
pp. 11122-11132 ◽  
Author(s):  
Mithun Roy ◽  
Biswarup Pathak ◽  
Ashis K. Patra ◽  
Eluvathingal D. Jemmis ◽  
Munirathinam Nethaji ◽  
...  
Keyword(s):  
Visible Light ◽  
Metal Ions ◽  
Dna Cleavage ◽  
Cleavage Activity ◽  
Dna Cleavage Activity
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