Visual detection of single-nucleotide polymorphisms and DNA methyltransferase based on cation-exchange of CuS nanoparticles and click chemistry of functionalized gold nanoparticles

2016 ◽  
Vol 52 (90) ◽  
pp. 13261-13264 ◽  
Author(s):  
X. R. Zhang ◽  
Y. Zhang ◽  
F. T. Chen ◽  
Y. Li ◽  
S. S. Zhang
Keyword(s):  
Gold Nanoparticles ◽  
Single Nucleotide Polymorphisms ◽  
Cation Exchange ◽  
Click Chemistry ◽  
Dna Methyltransferase ◽  
Visual Detection ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Functionalized Gold Nanoparticles

A novel biosensor was developed based on the cation-exchange of CuS nanoparticles (NPs) and Cu(i)-based click chemistry of functionalized gold nanoparticles (AuNPs).

Amplified electrochemical genotyping of single-nucleotide polymorphisms using a graphene–gold nanoparticles modified glassy carbon platform

RSC Advances ◽  
2015 ◽  
Vol 5 (37) ◽  
pp. 29285-29293 ◽  
Author(s):  
Seyyed Mehdi Khoshfetrat ◽  
Masoud A. Mehrgardi
Keyword(s):  
Gold Nanoparticles ◽  
Single Nucleotide Polymorphisms ◽  
Glassy Carbon ◽  
Signal Amplification ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Silver And Gold Nanoparticles ◽  
Amplification Strategy

A triple signal amplification strategy for the single nucleotide polymorphisms (SNPs) genotyping is reported using the graphene–gold nanoparticles (GR–AuNPs) as a sensitive platform and monobase-modified silver and gold nanoparticles (M-NPs).

Reusable Electrochemical Biosensor for Sensitive Detection of Single Nucleotide Polymorphisms Based on Gold Nanoparticle Self-Assembly

2014 ◽  
Vol 651-653 ◽  
pp. 293-296
Author(s):  
Song Bai Zhang ◽  
Chun Jiao Tang ◽  
Liao Yong Luo ◽  
Na Liu ◽  
Qin Li Sun ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Single Nucleotide Polymorphisms ◽  
Gold Nanoparticle ◽  
Self Assembly ◽  
Gold Electrode ◽  
Sensitive Detection ◽  
Capture Probe ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Target Dna

A reusable electrochemical biosensing strategy based on gold nanoparticle involved layer-by-layer self-assembly for sensitive detection of single nucleotide polymorphisms is proposed in this study. Making use of the strong sulfur-Au affinity, ethanthiol and capture probe modified gold nanoparticles are self-assembled onto the surface of gold electrode successively. The target DNA hybridizes with the capture probe and ferrocene labeled signaling probe successively via sandwich hybridization reaction. By measuring ac current voltammetry, the target DNA can be sensitively detected in a linear dynamic range from 4.1-410 nM with a low detection limit of 2 nM. Making use of self-assembled gold nanoparticles layer, a large amount of capture probes can be modified onto the gold electrode, supporting the high sensitivity of the proposed strategy. In addition, good reproducibility, high selectivity and stability are achieved. In particular, the biosensor can be easily regenerated by melting in hot water, making it reusable.

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