Amplified electrochemical genotyping of single-nucleotide polymorphisms using a graphene–gold nanoparticles modified glassy carbon platform

A triple signal amplification strategy for the single nucleotide polymorphisms (SNPs) genotyping is reported using the graphene–gold nanoparticles (GR–AuNPs) as a sensitive platform and monobase-modified silver and gold nanoparticles (M-NPs).

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Ligase chain reaction-based electrochemical biosensor for the ultrasensitive and specific detection of single nucleotide polymorphisms

New Journal of Chemistry ◽

10.1039/c9nj03994e ◽

2019 ◽

Vol 43 (36) ◽

pp. 14327-14335 ◽

Cited By ~ 3

Author(s):

Wancun Zhang ◽

Fang Hu ◽

Xianwei Zhang ◽

Wei Meng ◽

Yaodong Zhang ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Signal Amplification ◽

Electrochemical Biosensor ◽

Nucleotide Polymorphisms ◽

Specific Detection ◽

Chain Reaction ◽

Single Nucleotide ◽

Ligase Chain Reaction ◽

Amplification Strategy

In this study, a sensitive electrochemical biosensor for universally, robustly, specifically, and sensitively detecting SNPs was developed by using LCR as a signal amplification strategy.

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Visual detection of single-nucleotide polymorphisms and DNA methyltransferase based on cation-exchange of CuS nanoparticles and click chemistry of functionalized gold nanoparticles

Chemical Communications ◽

10.1039/c6cc06735b ◽

2016 ◽

Vol 52 (90) ◽

pp. 13261-13264 ◽

Cited By ~ 13

Author(s):

X. R. Zhang ◽

Y. Zhang ◽

F. T. Chen ◽

Y. Li ◽

S. S. Zhang

Keyword(s):

Gold Nanoparticles ◽

Single Nucleotide Polymorphisms ◽

Cation Exchange ◽

Click Chemistry ◽

Dna Methyltransferase ◽

Visual Detection ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Functionalized Gold Nanoparticles

A novel biosensor was developed based on the cation-exchange of CuS nanoparticles (NPs) and Cu(i)-based click chemistry of functionalized gold nanoparticles (AuNPs).

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Gold Nanoparticles - based Colorimetric Single Nucleotide Polymorphisms Genotyping Utilizing Allele-specific PCR

IFMBE Proceedings - 5th European Conference of the International Federation for Medical and Biological Engineering ◽

10.1007/978-3-642-23508-5_276 ◽

2011 ◽

pp. 1062-1065

Author(s):

Ye Lim Jung ◽

Cheulhee Jung ◽

Harshala Parab ◽

Hyun Gyu Park

Keyword(s):

Gold Nanoparticles ◽

Single Nucleotide Polymorphisms ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Specific Pcr ◽

Allele Specific ◽

Allele Specific Pcr

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A gold nanoparticles-based colorimetric test to detect single nucleotide polymorphisms for improvement of personalized therapy of psoriasis

10.1117/12.2225930 ◽

2016 ◽

Cited By ~ 2

Author(s):

Alessandra Marsella ◽

Paola Valentini ◽

Paolo Tarantino ◽

Maurizio Congedo ◽

Pier Paolo Pompa

Keyword(s):

Gold Nanoparticles ◽

Single Nucleotide Polymorphisms ◽

Personalized Therapy ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Colorimetric Test

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Study of Single-Stranded DNA Binding Protein–Nucleic Acids Interactions using Unmodified Gold Nanoparticles and Its Application for Detection of Single Nucleotide Polymorphisms

Analytical Chemistry ◽

10.1021/ac200525a ◽

2011 ◽

Vol 83 (11) ◽

pp. 4251-4257 ◽

Cited By ~ 58

Author(s):

Yen Nee Tan ◽

Kwai Han Lee ◽

Xiaodi Su

Keyword(s):

Gold Nanoparticles ◽

Dna Binding ◽

Single Nucleotide Polymorphisms ◽

Nucleic Acids ◽

Binding Protein ◽

Dna Binding Protein ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Single Stranded Dna

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Reusable Electrochemical Biosensor for Sensitive Detection of Single Nucleotide Polymorphisms Based on Gold Nanoparticle Self-Assembly

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.651-653.293 ◽

2014 ◽

Vol 651-653 ◽

pp. 293-296

Author(s):

Song Bai Zhang ◽

Chun Jiao Tang ◽

Liao Yong Luo ◽

Na Liu ◽

Qin Li Sun ◽

...

Keyword(s):

Gold Nanoparticles ◽

Single Nucleotide Polymorphisms ◽

Gold Nanoparticle ◽

Self Assembly ◽

Gold Electrode ◽

Sensitive Detection ◽

Capture Probe ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Target Dna

A reusable electrochemical biosensing strategy based on gold nanoparticle involved layer-by-layer self-assembly for sensitive detection of single nucleotide polymorphisms is proposed in this study. Making use of the strong sulfur-Au affinity, ethanthiol and capture probe modified gold nanoparticles are self-assembled onto the surface of gold electrode successively. The target DNA hybridizes with the capture probe and ferrocene labeled signaling probe successively via sandwich hybridization reaction. By measuring ac current voltammetry, the target DNA can be sensitively detected in a linear dynamic range from 4.1-410 nM with a low detection limit of 2 nM. Making use of self-assembled gold nanoparticles layer, a large amount of capture probes can be modified onto the gold electrode, supporting the high sensitivity of the proposed strategy. In addition, good reproducibility, high selectivity and stability are achieved. In particular, the biosensor can be easily regenerated by melting in hot water, making it reusable.

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