Regulation of MAP4K4 gene expression by RNA interference through an engineered theophylline-dependent hepatitis delta virus ribozyme switch

2016 ◽  
Vol 12 (11) ◽  
pp. 3370-3376 ◽  
Author(s):  
Hui Cheng ◽  
Yuanyuan Zhang ◽  
Hongyan Wang ◽  
Na Sun ◽  
Min Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Interference ◽  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
New Approach ◽  
Effector Domain ◽  
Hdv Ribozyme ◽  
Gene Regulatory ◽  
Inducible Rnai ◽  
Delta Virus

We chose the HDV ribozyme with an embedded theophylline aptamer as the sensor domain and the pri-miRNA as the effector domain to engineer a gene-regulatory device, and developed a new approach for inducible RNAi in the control of MAP4K4 gene expression.

scholarly journals Structural Landmarks of the Hepatitis Delta Virus (HDV) Ribozyme

2011 ◽  
Vol 100 (3) ◽  
pp. 236a
Author(s):  
Kamali Sripathi ◽  
Pavel Banáš ◽  
Jiří Šponer ◽  
Michal Otyepka ◽  
Nils Walter
Keyword(s):  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
Hdv Ribozyme ◽  
Delta Virus

scholarly journals Site-specific modification of functional groups in genomic hepatitis delta virus (HDV) ribozyme

2002 ◽  
Vol 269 (23) ◽  
pp. 5792-5803 ◽  
Author(s):  
Fumiko Nishikawa ◽  
Miho Shirai ◽  
Satoshi Nishikawa
Keyword(s):  
Functional Groups ◽  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
Site Specific ◽  
Specific Modification ◽  
Hdv Ribozyme ◽  
Delta Virus

scholarly journals Controlling Mammalian Gene Expression by Allosteric Hepatitis Delta Virus Ribozymes

2013 ◽  
Vol 2 (12) ◽  
pp. 684-689 ◽  
Author(s):  
Yoko Nomura ◽  
Linlin Zhou ◽  
Anh Miu ◽  
Yohei Yokobayashi
Keyword(s):  
Gene Expression ◽  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
Mammalian Gene ◽  
Mammalian Gene Expression ◽  
Delta Virus

Mechanistic Analysis of the Hepatitis Delta Virus (HDV) Ribozyme: Methods for RNA Preparation, Structure Mapping, Solvent Isotope Effects, and Co-transcriptional Cleavage

2012 ◽  
pp. 21-40 ◽  
Author(s):  
Durga M. Chadalavada ◽  
Andrea L. Cerrone-Szakal ◽  
Jennifer L. Wilcox ◽  
Nathan A. Siegfried ◽  
Philip C. Bevilacqua
Keyword(s):  
Hepatitis Delta Virus ◽  
Isotope Effects ◽  
Structure Mapping ◽  
Hepatitis Delta ◽  
Mechanistic Analysis ◽  
Hdv Ribozyme ◽  
Solvent Isotope ◽  
Solvent Isotope Effects ◽  
Delta Virus

scholarly journals Infectious Clones of Tomato Chlorosis Virus: Toward Increasing Efficiency by Introducing the Hepatitis Delta Virus Ribozyme

2021 ◽  
Vol 12 ◽  
Author(s):  
Elisa Navas-Hermosilla ◽  
Elvira Fiallo-Olivé ◽  
Jesús Navas-Castillo
Keyword(s):  
Hepatitis Delta Virus ◽  
Reverse Genetic ◽  
Genetic Studies ◽  
Hepatitis Delta ◽  
Tomato Plants ◽  
Infectious Clones ◽  
Viral Progeny ◽  
Hdv Ribozyme ◽  
Tomato Chlorosis Virus ◽  
Delta Virus

Tomato chlorosis virus (ToCV) is an emergent plant pathogen that causes a yellow leaf disorder in tomato and other solanaceous crops. ToCV is a positive-sense, single stranded (ss)RNA bipartite virus with long and flexuous virions belonging to the genus Crininivirus (family Closteroviridae). ToCV is phloem-limited, transmissible by whiteflies, and causes symptoms of interveinal chlorosis, bronzing, and necrosis in the lower leaves of tomato accompanied by a decline in vigor and reduction in fruit yield. The availability of infectious virus clones is a valuable tool for reverse genetic studies that has been long been hampered in the case of closterovirids due to their genome size and complexity. Here, attempts were made to improve the infectivity of the available agroinfectious cDNA ToCV clones (isolate AT80/99-IC from Spain) by adding the hepatitis delta virus (HDV) ribozyme fused to the 3′ end of both genome components, RNA1 and RNA2. The inclusion of the ribozyme generated a viral progeny with RNA1 3′ ends more similar to that present in the clone used for agroinoculation. Nevertheless, the obtained clones were not able to infect tomato plants by direct agroinoculation, like the original clones. However, the infectivity of the clones carrying the HDV ribozyme in Nicotiana benthamiana plants increased, on average, by two-fold compared with the previously available clones.

scholarly journals Prevalence of hepatitis delta virus among chronic hepatitis B carriers in a large tertiary center in the Netherlands

2021 ◽  
pp. 104870
Author(s):  
Boris J.B. Beudeker ◽  
Jolanda J.C. Voermans ◽  
Corine.H. GeurtsvanKessel ◽  
Robert J. de Knegt ◽  
Tamara Kuhlemann ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
The Netherlands ◽  
Chronic Hepatitis B ◽  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
Tertiary Center ◽  
Delta Virus

scholarly journals Replicating hepatitis delta virus RNA is edited in the nucleus by the small form of ADAR1

2002 ◽  
Vol 99 (23) ◽  
pp. 15118-15123 ◽  
Author(s):  
S. K. Wong ◽  
D. W. Lazinski
Keyword(s):  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
Small Form ◽  
Virus Rna ◽  
Delta Virus

scholarly journals Analysis of Multiple Liver Explant Pieces Reveals that Levels of Hepatitis Delta Virus RNA Are Independent of Hepatitis B Virus Expression

2021 ◽  
Author(s):  
Kasthuri Prakash ◽  
Simon B. Larsson ◽  
Gustaf E. Rydell ◽  
Johan Ringlander ◽  
Catarina Skoglund ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
Virus Rna ◽  
B Virus ◽  
Virus Expression ◽  
Delta Virus
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