scholarly journals iTRAQ-based proteomic technology revealed protein perturbations in intestinal mucosa from manganese exposure in rat models

RSC Advances ◽  
2017 ◽  
Vol 7 (50) ◽  
pp. 31745-31758 ◽  
Author(s):  
Hui Wang ◽  
Shengyi Wang ◽  
Dongan Cui ◽  
Shuwei Dong ◽  
Xin Tuo ◽  
...  
Keyword(s):  
Intestinal Mucosa ◽  
Metal Ion ◽  
Cellular Level ◽  
Essential Metal ◽  
Rat Models ◽  
Proteomic Technology ◽  
Manganese Exposure ◽  
Homeostatic Mechanisms

Manganese (Mn) is an essential metal ion as a biological cofactor, but in excess, it is toxic; however, the homeostatic mechanisms of Mn at the cellular level have not been identified.

scholarly journals The Dual Antimelanogenic and Antioxidant Activities of the Essential Oil Extracted from the Leaves ofAcorus macrospadiceus(Yamamoto) F. N. Wei et Y. K. Li

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Huey-Chun Huang ◽  
Hsiao-Fen Wang ◽  
Kuang-Hway Yih ◽  
Long-Zen Chang ◽  
Tsong-Min Chang
Keyword(s):  
Essential Oil ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Reducing Power ◽  
Cellular Level ◽  
Tyrosinase Activity ◽  
Oxygen Species ◽  
Melanin Content ◽  
Chelating Activity

The antimelanogenic and antioxidant activities of the essential oil extracted from the leaves ofAcorus macrospadiceus(Yamamoto) F. N. Wei et Y. K. Li have never been explored. The essential oil effectively inhibited mushroom tyrosinase activity (EC50= 1.57 mg/mL) and B16F10 tyrosinase activity (IC50= 1.01 mg/mL), decreased the melanin content (EC50= 1.04 mg/mL), and depleted the cellular level of the reactive oxygen species (ROS) (EC50= 1.87 mg/mL). The essential oil effectively scavenged 2,2-diphenyl-1-picryl-hydrazyl (DPPH) (EC50= 0.121 mg/mL) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) ABTS+radicals (EC50= 0.122 mg/mL). It also exhibited an apparent reducing power (EC50= 0.021 mg/mL) and metal-ion chelating activity (EC50= 0.029 mg/mL). The chemical constituents of the essential oil are ethers (55.73%), ketones (19.57%), monoterpenes (7.82%), alcohols (3.85%), esters (3.77%), sesquiterpenes (3.72%), and aromatic compounds (2.85%). The results confirm thatA. macrospadiceusessential oil is a natural antioxidant and inhibitor of melanogenesis.

Eliminating Competition: Characterizing and Eliminating Competitive Binding at Separate Sites between DAHP Synthase’s Essential Metal Ion and the Inhibitor DAHP Oxime

Biochemistry ◽  
2018 ◽  
Vol 57 (48) ◽  
pp. 6679-6687 ◽  
Author(s):  
Maren Heimhalt ◽  
Shan Jiang ◽  
Paul J. Berti
Keyword(s):  
Metal Ion ◽  
Competitive Binding ◽  
Essential Metal

scholarly journals Reconstitution of a thermophilic Cu+ importer in vitro reveals intrinsic high-affinity slow transport driving accumulation of an essential metal ion

2018 ◽  
Vol 293 (40) ◽  
pp. 15497-15512 ◽  
Author(s):  
Brandon L. Logeman ◽  
Dennis J. Thiele
Keyword(s):  
Metal Ion ◽  
Essential Metal ◽  
High Affinity ◽  
Slow Transport

scholarly journals Gestational Cd Exposure in the CD-1 Mouse Sex-Specifically Disrupts Essential Metal Ion Homeostasis

2021 ◽  
Author(s):  
Thomas W Jackson ◽  
Oliver Baars ◽  
Scott M Belcher
Keyword(s):  
Metal Ion ◽  
Ion Homeostasis ◽  
Female Offspring ◽  
Hepatic Insulin Resistance ◽  
Liver Iron ◽  
Metal Concentrations ◽  
Essential Metal ◽  
Metal Ion Homeostasis ◽  
Placental Efficiency ◽  
Metabolic Disruption

In CD-1 mice, gestational-only exposure to cadmium (Cd) causes female-specific hepatic insulin resistance, metabolic disruption, and obesity. To evaluate whether sex differences in cadmium uptake and changes in essential metal concentrations contribute to metabolic outcomes, placental and liver cadmium and essential metal concentrations were quantified in male and female offspring perinatally exposed to 500 ppb CdCl2. Exposure resulted in increased maternal liver Cd+2 concentrations (364 microgram/kg) similar to concentrations found in non-occupationally exposed human liver. At gestational day (GD) 18, placental cadmium and manganese concentrations were significantly increased in exposed males and females, and zinc was significantly decreased in females. Placental efficiency was significantly decreased in GD18 exposed males. Increases in hepatic Cd concentrations and a transient prenatal increase in zinc were observed in exposed female liver. Fetal and adult liver iron concentrations were decreased in both sexes, and decreases in hepatic zinc, iron, and manganese were observed in exposed females. Analysis of GD18 placental and liver metallothionein mRNA expression revealed significant Cd-induced upregulation of placental metallothionein in both sexes, and a significant decrease in fetal hepatic metallothionein in exposed females. In placenta, expression of metal ion transporters responsible for metal ion uptake was increased in exposed females. In liver of exposed adult female offspring, expression of the divalent cation importer (Slc39a14/Zip14) decreased, whereas expression of the primary exporter (Slc30a10) increased. These findings demonstrate that Cd can preferentially cross the female placenta, accumulate in the liver, and cause lifelong dysregulation of metal ion concentrations associated with metabolic disruption.

Bright lights down under: Metal ion complexes turning the spotlight on metabolic processes at the cellular level

2018 ◽  
Vol 375 ◽  
pp. 234-255 ◽  
Author(s):  
Todd A. Gillam ◽  
Martin J. Sweetman ◽  
Christie A. Bader ◽  
Janna L. Morrison ◽  
John D. Hayball ◽  
...  
Keyword(s):  
Metal Ion ◽  
Cellular Level ◽  
Metabolic Processes ◽  
Metal Ion Complexes

Age-related changes in small intestinal mucosa epithelium architecture and epithelial tight junction in rat models

2013 ◽  
Vol 26 (2) ◽  
pp. 183-191 ◽  
Author(s):  
Wei-ying Ren ◽  
Ke-fen Wu ◽  
Xi Li ◽  
Man Luo ◽  
Hong-chun Liu ◽  
...  
Keyword(s):  
Tight Junction ◽  
Intestinal Mucosa ◽  
Small Intestinal Mucosa ◽  
Rat Models ◽  
Age Related ◽  
Epithelial Tight Junction ◽  
Small Intestinal ◽  
Mucosa Epithelium ◽  
Age Related Changes

RNAs' uracil quartet model with a non-essential metal ion

2011 ◽  
Vol 47 (16) ◽  
pp. 4646 ◽  
Author(s):  
Miquel Barceló-Oliver ◽  
Carolina Estarellas ◽  
Àngel Terrón ◽  
Ángel García-Raso ◽  
Antonio Frontera
Keyword(s):  
Metal Ion ◽  
Essential Metal

scholarly journals Physical Interaction and Functional Coupling between ACDP4 and the Intracellular Ion Chaperone COX11, an Implication of the Role of ACDP4 in Essential Metal Ion Transport and Homeostasis

2005 ◽  
Vol 1 ◽  
pp. 1744-8069-1-15 ◽  
Author(s):  
Dehuang Guo ◽  
Jennifer Ling ◽  
Mong-Heng Wang ◽  
Jin-Xiong She ◽  
Jianguo Gu ◽  
...  
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Spinal Cord Dorsal Horn ◽  
Hek293 Cells ◽  
Functional Coupling ◽  
Essential Metal ◽  
Dorsal Horn Neurons ◽  
Copper Manganese ◽  
Spinal Cord Dorsal

Divalent metal ions such as copper, manganese, and cobalt are essential for cell development, differentiation, function and survival. These essential metal ions are delivered into intracellular domains as cofactors for enzymes involved in neuropeptide and neurotransmitter synthesis, superoxide metabolism, and other biological functions in a target specific fashion. Altering the homeostasis of these essential metal ions is known to connect to a number of human diseases including Alzheimer disease, amyotrophic lateral sclerosis, and pain. It remains unclear how these essential metal ions are delivered to intracellular targets in mammalian cells. Here we report that rat spinal cord dorsal horn neurons express ACDP4, a member of Ancient Conserved Domain Protein family. By screening a pretransformed human fetal brain cDNA library in a yeast two-hybrid system, we have identified that ACDP4 specifically interacts with COX11, an intracellular metal ion chaperone. Ectopic expression of ACDP4 in HEK293 cells resulted in enhanced toxicity to metal ions including copper, manganese, and cobalt. The metal ion toxicity became more pronounced when ACDP4 and COX11 were co-expressed ectopically in HEK293 cells, suggesting a functional coupling between them. Our results indicate a role of ACDP4 in metal ion homeostasis and toxicity. This is the first report revealing a functional aspect of this ancient conserved domain protein family. We propose that ACDP is a family of transporter protein or chaperone proteins for delivering essential metal ions in different mammalian tissues. The expression of ACDP4 on spinal cord dorsal horn neurons may have implications in sensory neuron functions under physiological and pathological conditions.

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