Enhancing intracellular microRNA imaging: a new strategy combining double-channel exciting single colour fluorescence with the target cycling amplification reaction

2018 ◽  
Vol 54 (93) ◽  
pp. 13131-13134 ◽  
Author(s):  
Keying Zhang ◽  
Shuting Song ◽  
Lin Yang ◽  
Qianhao Min ◽  
Xingcai Wu ◽  
...  
Keyword(s):  
Living Cells ◽  
New Strategy ◽  
Single Colour ◽  
Amplification Reaction ◽  
Double Channel

Enhancing microRNA imaging in living cells using double-channel exciting single colour fluorescence coupled with the target cycling amplification reaction.

Sensitive and sustained imaging of intracellular microRNA in living cells by a high biocompatible liposomal vehicle introduced isothermal symmetric exponential amplification reaction

2019 ◽  
Vol 55 (75) ◽  
pp. 11251-11254 ◽  
Author(s):  
Wen Yin ◽  
Jun Chen ◽  
Huihui Yang ◽  
Yanfei Zhang ◽  
Zong Dai ◽  
...  
Keyword(s):  
Living Cells ◽  
Highly Efficient ◽  
Amplification Reaction

A biocompatible liposome was fabricated to introduce highly efficient oligonucleotide amplification in living cells for the sensitive and sustained imaging of microRNA.

Ultrafast and reversibly cyclization/cycloreversion of 4-imino-4H-Quinolizine-1-Carbonitrile: A new strategy for fluorescence modulation in pH sensing in living cells

2021 ◽  
pp. 109694
Author(s):  
Tao Yang ◽  
Huidong Li ◽  
Zhixin Cao ◽  
Yang Yang ◽  
Dasheng Zheng ◽  
...  
Keyword(s):  
Living Cells ◽  
Ph Sensing ◽  
New Strategy ◽  
Fluorescence Modulation

Biomineralized Zeolitic Imidazolate Framework-8 Nanoparticles Enable Polymerase/Endonuclease Synergetic Amplification Reaction in Living Cells for Sensitive MicroRNA Imaging

2021 ◽  
Author(s):  
Ke Zhang ◽  
Xiayun Le ◽  
Qiaoqin Yu ◽  
Juan Zhang ◽  
Dandan Wang ◽  
...  
Keyword(s):  
Living Cells ◽  
Zeolitic Imidazolate Framework ◽  
Amplification Reaction

In this work, we have developed a simple-prepared nanocomplex probe, which could implement polymerase and endonuclease synergetic amplification reaction in living cells by the biomineralized ZIF-8 NPs for intracellular miRNA...

Tricking Enzymes In Living Cells: A Mechanism-Based Strategy For Design of DNA Topoisomerase Biosensors

2021 ◽  
Author(s):  
Sai Ba ◽  
Guangpeng Gao ◽  
Tianhu Li ◽  
Hao Zhang
Keyword(s):  
Design Strategy ◽  
Living Cells ◽  
Molecular Probes ◽  
Chemical Bonds ◽  
Dna Topoisomerase ◽  
Dna Topoisomerases ◽  
Catalytic Activities ◽  
New Strategy ◽  
Clinical Tools ◽  
Catalytic Cycles

Abstract Most activity-based molecular probes are designed to target enzymes that catalyze the breaking of chemical bonds and the conversion of a unimolecular substrate into bimolecular products. However, DNA topoisomerases are a class of enzymes that alter DNA topology without producing any molecular segments during catalysis, which hinders the development of practical methods for diagnosing these key biomarkers in living cells. Here, we established a new strategy for the effective sensing of the expression levels and catalytic activities of topoisomerases in cell-free systems and human cells. Using our newly designed biosensors, we tricked DNA topoisomerases within their catalytic cycles to switch on fluorescence and resume new rounds of catalysis. Considering that human topoisomerases have been widely recognized as biomarkers for multiple cancers and identified as promising targets for several anticancer drugs, we believe that these DNA-based biosensors and our design strategy would greatly benefit the future development of clinical tools for cancer diagnosis and treatment.

scholarly journals Tricking enzymes in living cells: a mechanism-based strategy for design of DNA topoisomerase biosensors

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sai Ba ◽  
Guangpeng Gao ◽  
Tianhu Li ◽  
Hao Zhang
Keyword(s):  
Design Strategy ◽  
Living Cells ◽  
Molecular Probes ◽  
Chemical Bonds ◽  
Dna Topoisomerase ◽  
Dna Topoisomerases ◽  
Catalytic Activities ◽  
New Strategy ◽  
Clinical Tools ◽  
Catalytic Cycles

AbstractMost activity-based molecular probes are designed to target enzymes that catalyze the breaking of chemical bonds and the conversion of a unimolecular substrate into bimolecular products. However, DNA topoisomerases are a class of enzymes that alter DNA topology without producing any molecular segments during catalysis, which hinders the development of practical methods for diagnosing these key biomarkers in living cells. Here, we established a new strategy for the effective sensing of the expression levels and catalytic activities of topoisomerases in cell-free systems and human cells. Using our newly designed biosensors, we tricked DNA topoisomerases within their catalytic cycles to switch on fluorescence and resume new rounds of catalysis. Considering that human topoisomerases have been widely recognized as biomarkers for multiple cancers and identified as promising targets for several anticancer drugs, we believe that these DNA-based biosensors and our design strategy would greatly benefit the future development of clinical tools for cancer diagnosis and treatment. Graphical Abstract

A new strategy to improve the water solubility of an organic fluorescent probe using silicon nanodots and fabricate two-photon SiND-ANPA-N3 for visualizing hydrogen sulfide in living cells and onion tissues

2020 ◽  
Vol 8 (7) ◽  
pp. 1422-1431 ◽  
Author(s):  
Yu-Jia Fu ◽  
San-San Shen ◽  
Xiao-Feng Guo ◽  
Hong Wang
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Water Solubility ◽  
Aqueous Media ◽  
Living Cells ◽  
Water Soluble ◽  
Two Photon ◽  
New Strategy

A water-soluble fluorescent probe based on SiNDs for H2S detection can be used in both fully aqueous media and living cells.

A new strategy for fabricating organic photovoltaic devices with stable D/A double-channel network to enhance performance using self-assembling all-conjugated diblock copolymer

Nano Energy ◽  
2015 ◽  
Vol 13 ◽  
pp. 103-116 ◽  
Author(s):  
Yi-Huan Lee ◽  
Wei-Chih Chen ◽  
Chi-Ju Chiang ◽  
Kuo-Chang Kau ◽  
Wei-Shin Liou ◽  
...  
Keyword(s):  
Diblock Copolymer ◽  
Organic Photovoltaic ◽  
Photovoltaic Devices ◽  
Organic Photovoltaic Devices ◽  
Channel Network ◽  
Self Assembling ◽  
New Strategy ◽  
Double Channel

Monitoring Telomerase Activity in Living Cells with High Sensitivity Using Cascade Amplification Reaction-Based Nanoprobe

2019 ◽  
Vol 91 (20) ◽  
pp. 13143-13151 ◽  
Author(s):  
Huanhuan Fan ◽  
Huarong Bai ◽  
Qin Liu ◽  
Hang Xing ◽  
Xiao-Bing Zhang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Telomerase Activity ◽  
Living Cells ◽  
Cascade Amplification ◽  
Amplification Reaction

A remarkable colorimetric probe for fluorescent ratiometric and ON-OFF discriminative detection of Hg2+ and Cu2+ by double-channel imaging in living cells

2019 ◽  
Vol 171 ◽  
pp. 107782 ◽  
Author(s):  
Wen-Jing Shi ◽  
Chun-Feng Li ◽  
Yan Huang ◽  
Hui-ya Tan ◽  
Yong-Feng Wei ◽  
...  
Keyword(s):  
Living Cells ◽  
Colorimetric Probe ◽  
Double Channel
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