Sensitive and sustained imaging of intracellular microRNA in living cells by a high biocompatible liposomal vehicle introduced isothermal symmetric exponential amplification reaction

2019 ◽  
Vol 55 (75) ◽  
pp. 11251-11254 ◽  
Author(s):  
Wen Yin ◽  
Jun Chen ◽  
Huihui Yang ◽  
Yanfei Zhang ◽  
Zong Dai ◽  
...  
Keyword(s):  
Living Cells ◽  
Highly Efficient ◽  
Amplification Reaction

A biocompatible liposome was fabricated to introduce highly efficient oligonucleotide amplification in living cells for the sensitive and sustained imaging of microRNA.

scholarly journals Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

RSC Advances ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Xuekang Cai ◽  
Dan Wang ◽  
Yasi Gao ◽  
Long Yi ◽  
Xing Yang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Living Cells ◽  
Highly Efficient ◽  
Aromatic Azide

A fast strain-promoted azide–alkyne cycloaddition based on tetra-fluorinated aromatic azide was developed and applied to label proteins and living cells with high efficiency.

scholarly journals A Responsive “Nano String Light” for Highly Efficient mRNA Imaging in Living Cells via Accelerated DNA Cascade Reaction

ACS Nano ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 263-271 ◽  
Author(s):  
Kewei Ren ◽  
Yifan Xu ◽  
Ying Liu ◽  
Min Yang ◽  
Huangxian Ju
Keyword(s):  
Living Cells ◽  
Cascade Reaction ◽  
Highly Efficient

Circular DNA: a stable probe for highly efficient mRNA imaging and gene therapy in living cells

2018 ◽  
Vol 54 (8) ◽  
pp. 896-899 ◽  
Author(s):  
Jingying Li ◽  
Jie Zhou ◽  
Tong Liu ◽  
Shan Chen ◽  
Juan Li ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Graphene Oxide ◽  
Living Cells ◽  
Circular Dna ◽  
Highly Efficient

We integrated circular DNA with graphene oxide to fabricate improved platforms for highly efficient imaging and therapy in living cells.

scholarly journals Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene

2020 ◽  
Author(s):  
Linda S. Forero-Quintero ◽  
William Raymond ◽  
Tetsuya Handa ◽  
Matthew Saxton ◽  
Tatsuya Morisaki ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Single Molecule ◽  
Spatial Organization ◽  
Fluorescent Antibody ◽  
Single Gene ◽  
Single Copy ◽  
Living Cells ◽  
Live Cell ◽  
Highly Efficient

The carboxyl-terminal domain of RNA polymerase II is dynamically phosphorylated during transcription in eukaryotic cells. While residue-specific phosphorylation has been mapped with exquisite spatial resolution along the 1D genome in a population of fixed cells using immunoprecipitation-based assays, the timing, kinetics, and spatial organization of phosphorylation along a single-copy gene have not yet been measured in living cells. Here, we achieve this by combining multi-color, single-molecule microscopy with fluorescent antibody-based probes that specifically bind to unphosphorylated and phosphorylated forms of endogenous RNAP2 in living cells. Applying this methodology to a single-copy HIV-1 reporter gene provides live-cell evidence for heterogeneity in the distribution of RNAP2 along the length of the gene as well as clusters of Serine 5 phosphorylated RNAP2 that form around active genes and are separated in both space and time from nascent mRNA synthesis. Computational models fit to our data determine that 5 to 40 RNAP2 cluster around the promoter of a gene during typical transcriptional bursts. Nearly all RNAP2 either arrive with Serine 5 phosphorylation or acquire the modification within a minute. Transcription from the cluster appears to be highly efficient, with nearly half of the clustered RNAP2 ultimately escaping the promoter in a minute or so to elongate a full-length mRNA in approximately five minutes. The highly dynamic and spatially organized concentrations of RNAP2 we observe support the notion of highly efficient transcription clusters that form around promoters and contain high concentrations of RNAP2 phosphorylated at Serine 5.

Highly efficient approach for hypochlorous acid sensing in water samples and living cells based on acylhydrazone Schiff base functionalized fluorescent probes

2017 ◽  
Vol 41 (20) ◽  
pp. 12250-12258 ◽  
Author(s):  
Zheng Yang ◽  
Yuanlong He ◽  
Xiangrong Liu ◽  
Shunsheng Zhao ◽  
Zaiwen Yang ◽  
...  
Keyword(s):  
Schiff Base ◽  
Fluorescent Probes ◽  
Water Samples ◽  
High Selectivity ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Efficient Approach ◽  
Highly Efficient

Five HClO probes were present with high selectivity and sensitivity. The properties and mechanism were investigated both experimentally and theoretically.

Biomineralized Zeolitic Imidazolate Framework-8 Nanoparticles Enable Polymerase/Endonuclease Synergetic Amplification Reaction in Living Cells for Sensitive MicroRNA Imaging

2021 ◽  
Author(s):  
Ke Zhang ◽  
Xiayun Le ◽  
Qiaoqin Yu ◽  
Juan Zhang ◽  
Dandan Wang ◽  
...  
Keyword(s):  
Living Cells ◽  
Zeolitic Imidazolate Framework ◽  
Amplification Reaction

In this work, we have developed a simple-prepared nanocomplex probe, which could implement polymerase and endonuclease synergetic amplification reaction in living cells by the biomineralized ZIF-8 NPs for intracellular miRNA...

A Highly Efficient Chemiluminescence Probe for the Detection of Singlet Oxygen in Living Cells

2017 ◽  
Vol 129 (39) ◽  
pp. 11955-11958 ◽  
Author(s):  
Nir Hananya ◽  
Ori Green ◽  
Rachel Blau ◽  
Ronit Satchi-Fainaro ◽  
Doron Shabat
Keyword(s):  
Singlet Oxygen ◽  
Living Cells ◽  
Highly Efficient

Development of high-speed and highly efficient butanol production systems from butyric acid with high density of living cells of Clostridium saccharoperbutylacetonicum

2012 ◽  
Vol 157 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Shun-ichi Baba ◽  
Yukihiro Tashiro ◽  
Hideaki Shinto ◽  
Kenji Sonomoto
Keyword(s):  
Butyric Acid ◽  
High Speed ◽  
Production Systems ◽  
Living Cells ◽  
High Density ◽  
Butanol Production ◽  
Highly Efficient

Enhancing intracellular microRNA imaging: a new strategy combining double-channel exciting single colour fluorescence with the target cycling amplification reaction

2018 ◽  
Vol 54 (93) ◽  
pp. 13131-13134 ◽  
Author(s):  
Keying Zhang ◽  
Shuting Song ◽  
Lin Yang ◽  
Qianhao Min ◽  
Xingcai Wu ◽  
...  
Keyword(s):  
Living Cells ◽  
New Strategy ◽  
Single Colour ◽  
Amplification Reaction ◽  
Double Channel

Enhancing microRNA imaging in living cells using double-channel exciting single colour fluorescence coupled with the target cycling amplification reaction.

Two cyanoethylene-based fluorescence probes for highly efficient cyanide detection and practical applications in drinking water and living cells

Talanta ◽  
2021 ◽  
pp. 122615
Author(s):  
Ting Peng ◽  
Shining Li ◽  
Yuping Zhou ◽  
Ruiyuan Liu ◽  
Jinqing Qu
Keyword(s):  
Drinking Water ◽  
Living Cells ◽  
Fluorescence Probes ◽  
Practical Applications ◽  
Highly Efficient
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