Non-invasive study of the three-dimensional structure of nanoporous triblock terpolymer membranes

Soft Matter ◽  
2018 ◽  
Vol 14 (48) ◽  
pp. 9750-9754 ◽  
Author(s):  
Daniel Zalami ◽  
Oliver Grimm ◽  
Felix H. Schacher ◽  
Uwe Gerken ◽  
Jürgen Köhler
Keyword(s):  
Single Particle ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Three Dimensional Structure ◽  
Non Invasive ◽  
3D Morphology ◽  
Particle Orbit ◽  
Orbit Tracking ◽  
Nanoporous Structures

Single-particle orbit tracking for characterising the 3d morphology of liquid-filled nanoporous structures.

The Three-dimensional structure of frozen-hydrated nudaurelia capensis β virus

1987 ◽  
Vol 45 ◽  
pp. 650-651
Author(s):  
N. H. Olson ◽  
T. S. Baker ◽  
Wu Bo Mu ◽  
J. E. Johnson ◽  
D. A. Hendry
Keyword(s):  
South African ◽  
Rna Virus ◽  
Carbon Film ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Electron Dose ◽  
Total Electron ◽  
Three Dimensional Structure ◽  
Negative Stain ◽  
Dimensional Reconstruction

Nudaurelia capensis β virus (NβV) is an RNA virus of the South African Pine Emperor moth, Nudaurelia cytherea capensis (Lepidoptera: Saturniidae). The NβV capsid is a T = 4 icosahedron that contains 60T = 240 subunits of the coat protein (Mr = 61,000). A three-dimensional reconstruction of the NβV capsid was previously computed from visions embedded in negative stain suspended over holes in a carbon film. We have re-examined the three-dimensional structure of NβV, using cryo-microscopy to examine the native, unstained structure of the virion and to provide a initial phasing model for high-resolution x-ray crystallographic studiesNβV was purified and prepared for cryo-microscopy as described. Micrographs were recorded ∼1 - 2 μm underfocus at a magnification of 49,000X with a total electron dose of about 1800 e-/nm2.

Sign in / Sign up

Export Citation Format

Share Document