DNAzyme-powered nucleic acid release from solid supports

2020 ◽  
Vol 56 (4) ◽  
pp. 647-650 ◽  
Author(s):  
Ting Cao ◽  
Yongcheng Wang ◽  
Ye Tao ◽  
Lexiang Zhang ◽  
Ying-Lin Zhou ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Polyacrylamide Gel ◽  
Solid Supports ◽  
Site Specific ◽  
Gel Beads ◽  
Acid Release ◽  
Dependent Site

Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays.

Excitation dependent site-specific luminescence and structure-optical property correlation of Lu2Sn2O7:Eu3+ nanoparticles

2020 ◽  
Vol 109 ◽  
pp. 110357
Author(s):  
Santosh K. Gupta ◽  
Mitzy A. Penilla Garcia ◽  
Yuanbing Mao
Keyword(s):  
Optical Property ◽  
Site Specific ◽  
Property Correlation ◽  
Dependent Site

scholarly journals Metal-mediated base pairs in parallel-stranded DNA

2017 ◽  
Vol 13 ◽  
pp. 2671-2681 ◽  
Author(s):  
Jens Müller
Keyword(s):  
Nucleic Acid ◽  
Complex Formation ◽  
Transition Metal Ions ◽  
Dna Duplexes ◽  
Base Pairs ◽  
Experimental Conditions ◽  
Site Specific ◽  
Glycosidic Bonds ◽  
Double Helices ◽  
Significant Extension

In nucleic acid chemistry, metal-mediated base pairs represent a versatile method for the site-specific introduction of metal-based functionality. In metal-mediated base pairs, the hydrogen bonds between complementary nucleobases are replaced by coordinate bonds to one or two transition metal ions located in the helical core. In recent years, the concept of metal-mediated base pairing has found a significant extension by applying it to parallel-stranded DNA duplexes. The antiparallel-stranded orientation of the complementary strands as found in natural B-DNA double helices enforces a cisoid orientation of the glycosidic bonds. To enable the formation of metal-mediated base pairs preferring a transoid orientation of the glycosidic bonds, parallel-stranded duplexes have been investigated. In many cases, such as the well-established cytosine–Ag(I)–cytosine base pair, metal complex formation is more stabilizing in parallel-stranded DNA than in antiparallel-stranded DNA. This review presents an overview of all metal-mediated base pairs reported as yet in parallel-stranded DNA, compares them with their counterparts in regular DNA (where available), and explains the experimental conditions used to stabilize the respective parallel-stranded duplexes.

Unlockable Nanocomplexes with Self-Accelerating Nucleic Acid Release for Effective Staged Gene Therapy of Cardiovascular Diseases

2018 ◽  
Vol 30 (31) ◽  
pp. 1801570 ◽  
Author(s):  
Jing-Jun Nie ◽  
Bokang Qiao ◽  
Shun Duan ◽  
Chen Xu ◽  
Boya Chen ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Nucleic Acid ◽  
Cardiovascular Diseases ◽  
Acid Release

Development of simplified and rapid nucleic acid release protocol for PCR based detection of Potato viruses

2018 ◽  
Vol 46 (2) ◽  
pp. 255-262 ◽  
Author(s):  
Baswaraj Raigond ◽  
Ambika Verma ◽  
Tarvinder Kochhar ◽  
Shivani Roach ◽  
Sanjeev Sharma ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Potato Viruses ◽  
Acid Release

scholarly journals Site-specific conjugation of recognition tags to trastuzumab for peptide nucleic acid-mediated radionuclide HER2 pretargeting

Biomaterials ◽  
2019 ◽  
Vol 203 ◽  
pp. 73-85 ◽  
Author(s):  
Kristina Westerlund ◽  
Anzhelika Vorobyeva ◽  
Bogdan Mitran ◽  
Anna Orlova ◽  
Vladimir Tolmachev ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Site Specific

Comparison of site-specific coupling chemistry for antibody immobilization on different solid supports

1991 ◽  
Vol 542 ◽  
pp. 41-54 ◽  
Author(s):  
Lin Jinn-Nan ◽  
Chang I-Nan ◽  
Joseph D. Andrade ◽  
James N. Herron ◽  
Douglas A. Christensen
Keyword(s):  
Antibody Immobilization ◽  
Solid Supports ◽  
Site Specific
Sign in / Sign up

Export Citation Format

Share Document