scholarly journals Single mutation in a novel bacterial LOV protein yields a singlet oxygen generator

2019 ◽  
Vol 18 (11) ◽  
pp. 2657-2660 ◽  
Author(s):  
Eleonora Consiglieri ◽  
Qianzhao Xu ◽  
Mikkel Bregnhøj ◽  
Michael Westberg ◽  
Peter R. Ogilby ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Blue Light ◽  
Single Mutation ◽  
Facultative Methylotroph ◽  
Blue Light Photoreceptor ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator

The first blue light photoreceptor isolated from Methylobacterium radiotolerans, a pink-pigmented facultative methylotroph, has been turned into an efficient singlet oxygen generator by introducing a single mutation.

scholarly journals Tailing miniSOG: Structural Bases of the Complex Photophysics of a Flavin-Binding Singlet Oxygen Photosensitizing Protein

2018 ◽  
Author(s):  
Joaquim Torra ◽  
Céline Lafaye ◽  
Luca Signor ◽  
Sylvain Aumonier ◽  
Cristina Flors ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Singlet Oxygen ◽  
Molecular Mechanism ◽  
Blue Light ◽  
Light And Electron Microscopy ◽  
Light Irradiation ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator ◽  
Flavin Binding

We have elucidated the molecular mechanism by which the photosensitizing protein miniSOG, initially a poor singlet oxygen generator, becomes an efficient singlet oxygen generator after prolonged blue-light irradiation and a useful tool in correlative light and electron microscopy (CLEM) experiments.<br>

scholarly journals Tailing miniSOG: Structural Bases of the Complex Photophysics of a Flavin-Binding Singlet Oxygen Photosensitizing Protein

2018 ◽  
Author(s):  
Joaquim Torra ◽  
Céline Lafaye ◽  
Luca Signor ◽  
Sylvain Aumonier ◽  
Cristina Flors ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Singlet Oxygen ◽  
Molecular Mechanism ◽  
Blue Light ◽  
Light And Electron Microscopy ◽  
Light Irradiation ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator ◽  
Flavin Binding

We have elucidated the molecular mechanism by which the photosensitizing protein miniSOG, initially a poor singlet oxygen generator, becomes an efficient singlet oxygen generator after prolonged blue-light irradiation and is a useful tool in correlative light and electron microscopy (CLEM) experiments.<br>

scholarly journals Bioengineering a light-responsive encapsulin nanoreactor: a potential tool for photodynamic therapy

2020 ◽  
Author(s):  
Dennis Diaz ◽  
Xavier Vidal ◽  
Anwar Sunna ◽  
Andrew Care
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Blue Light ◽  
Oxidation Reactions ◽  
Temporal Precision ◽  
Self Assembling ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator ◽  
Potential Tool

AbstractEncapsulins, a prokaryotic class of self-assembling protein nanocompartments, are being re-engineered to serve as ‘nanoreactors’ for the augmentation or creation of key biochemical reactions. However, approaches that allow encapsulin nanoreactors to be functionally activated with spatial and temporal precision is lacking. We report the construction of a light-responsive encapsulin nanoreactor for “on-demand” production of reactive oxygen species (ROS). Herein, encapsulins were loaded with the fluorescent flavoprotein mini-Singlet Oxygen Generator (miniSOG), a biological photosensitizer that is activated by blue-light to generate ROS, primarily singlet oxygen (1O2). We established that the nanocompartments stably encased miniSOG, and in response to blue-light were able to mediate the photoconversion of molecular oxygen into ROS. Using an in vitro model of lung cancer, ROS generated by the nanoreactor was shown to trigger photosensitized oxidation reactions that exerted a toxic effect on tumour cells, suggesting utility in photodynamic therapy. This encapsulin nanoreactor thus represents a platform for the light-controlled initiation and/or modulation of ROS-driven processes in biomedicine and biotechnology.

scholarly journals Tailing miniSOG: Structural Bases of the Complex Photophysics of a Flavin-Binding Singlet Oxygen Photosensitizing Protein

2018 ◽  
Author(s):  
Joaquim Torra ◽  
Céline Lafaye ◽  
Luca Signor ◽  
Sylvain Aumonier ◽  
Cristina Flors ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Singlet Oxygen ◽  
Molecular Mechanism ◽  
Blue Light ◽  
Light And Electron Microscopy ◽  
Light Irradiation ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator ◽  
Flavin Binding

We have elucidated the molecular mechanism by which the photosensitizing protein miniSOG, initially a poor singlet oxygen generator, becomes an efficient singlet oxygen generator after prolonged blue-light irradiation and a useful tool in correlative light and electron microscopy (CLEM) experiments.<br>

Parametric study of a mist jet-singlet oxygen generator.

2000 ◽  
Vol 12 (1/2) ◽  
pp. 136-137
Author(s):  
Shigeki MUTO ◽  
Yosuke KOBAYASHI ◽  
Yoshinobu TAKEKAWA ◽  
Masamori ENDO ◽  
Kenzo NANRI ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Parametric Study ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator

High pressure oxygen iodine laser based on twisted flow singlet oxygen generator

2001 ◽  
Author(s):  
B. Vyskubenko ◽  
A. Adamenkov ◽  
S. Ilyin ◽  
Yu. Kolobyanin ◽  
I. Krukovsky ◽  
...  
Keyword(s):  
High Pressure ◽  
Singlet Oxygen ◽  
Iodine Laser ◽  
Pressure Oxygen ◽  
Twisted Flow ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator ◽  
High Pressure Oxygen

ChemInform Abstract: Design and Properties of Porphyrin-Based Singlet Oxygen Generator

ChemInform ◽  
2016 ◽  
Vol 47 (18) ◽  
Author(s):  
Hyo-Geun Jeong ◽  
Myung-Seok Choi
Keyword(s):  
Singlet Oxygen ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator

Pressure scaling of an electro-discharge singlet oxygen generator (ED SOG)

2007 ◽  
Vol 40 (21) ◽  
pp. 6571-6582 ◽  
Author(s):  
O V Braginsky ◽  
A S Kovalev ◽  
D V Lopaev ◽  
O V Proshina ◽  
T V Rakhimova ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator

Numerical Investigation of Gas‐Liquid Reacting Flow in a Jet‐Type Singlet Oxygen Generator

2020 ◽  
Vol 43 (9) ◽  
pp. 1859-1865
Author(s):  
Chao Xu ◽  
Xi Chen ◽  
Tingting Liu ◽  
Ying Huai ◽  
Guangwen Chen
Keyword(s):  
Singlet Oxygen ◽  
Numerical Investigation ◽  
Reacting Flow ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator

Preliminary study of a centrifugal-flow singlet oxygen generator

2008 ◽  
Vol 38 (2) ◽  
pp. 199-203 ◽  
Author(s):  
W Shi ◽  
L Deng ◽  
H Yang ◽  
G Sha ◽  
C Zhang
Keyword(s):  
Singlet Oxygen ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator ◽  
Preliminary Study
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