DNA translocation to giant unilamellar vesicles during electroporation is independent of DNA size

DNA delivery into GUVs during electroporation is governed by bulk electrophoretic mobility implying a mechanism in which DNA molecules enter in their coiled conformation, as opposed to stochastic threading, through electro-pores.

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Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles

Chemical Communications ◽

10.1039/c9cc03277k ◽

2019 ◽

Vol 55 (62) ◽

pp. 9084-9087 ◽

Cited By ~ 3

Author(s):

Yusuke Sato ◽

Ken Komiya ◽

Ibuki Kawamata ◽

Satoshi Murata ◽

Shin-ichiro M. Nomura

Keyword(s):

Isothermal Amplification ◽

Dna Molecules ◽

Giant Unilamellar Vesicles ◽

Unilamellar Vesicles ◽

Artificial Dna

An artificial DNA circuit in giant unilamellar vesicles allows isothermal amplification of DNA by over 5000-fold within several hours.

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Elucidating the Membrane Dynamics and Encapsulation Mechanism of Large DNA Molecules Under Molecular Crowding Conditions Using Giant Unilamellar Vesicles

ACS Synthetic Biology ◽

10.1021/acssynbio.0c00360 ◽

2020 ◽

Vol 9 (10) ◽

pp. 2819-2827

Author(s):

Mamiko Tsugane ◽

Hiroaki Suzuki

Keyword(s):

Membrane Dynamics ◽

Molecular Crowding ◽

Dna Molecules ◽

Giant Unilamellar Vesicles ◽

Unilamellar Vesicles

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Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽

10.3390/life11070634 ◽

2021 ◽

Vol 11 (7) ◽

pp. 634

Author(s):

Ylenia Miele ◽

Gábor Holló ◽

István Lagzi ◽

Federico Rossi

Keyword(s):

Phospholipid Fatty Acid ◽

Enzymatic Reaction ◽

Stimuli Responsive ◽

Membrane Composition ◽

Giant Unilamellar Vesicles ◽

Physical Processes ◽

Unilamellar Vesicles ◽

Division Process ◽

The Origin Of Life ◽

Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

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Efficient Production of Monodisperse Giant Unilamellar Vesicles by Transferring Across the W-O Interface

2021 IEEE 34th International Conference on Micro Electro Mechanical Systems (MEMS) ◽

10.1109/mems51782.2021.9375159 ◽

2021 ◽

Author(s):

Ryota Ushiyama ◽

Hiroaki Suzuki

Keyword(s):

Efficient Production ◽

Giant Unilamellar Vesicles ◽

Unilamellar Vesicles

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Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir ◽

10.1021/acs.langmuir.0c02843 ◽

2021 ◽

Vol 37 (3) ◽

pp. 1082-1088

Author(s):

Chiho Kataoka-Hamai ◽

Kohsaku Kawakami

Keyword(s):

Giant Unilamellar Vesicles ◽

Unilamellar Vesicles

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Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals

Soft Matter ◽

10.1039/d0sm01684e ◽

2021 ◽

Author(s):

Peng Bao ◽

Daniel A. Paterson ◽

Sally A. Peyman ◽

J. Cliff Jones ◽

Jonathan A. T. Sandoe ◽

...

Keyword(s):

Liquid Crystals ◽

Nematic Liquid Crystals ◽

Double Emulsion ◽

Lyotropic Liquid Crystals ◽

Giant Unilamellar Vesicles ◽

Unilamellar Vesicles ◽

Emulsion Droplets ◽

Double Emulsion Droplets

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).

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