Snake venom characteristic peptides: novel fingerprints for species identification by sheathless capillary electrophoresis-electrospray ionization-mass spectrometry

The Analyst ◽  
2020 ◽  
Vol 145 (14) ◽  
pp. 5027-5031
Author(s):  
Ying Liu ◽  
Xiao-Hui Zhang ◽  
Yue Yu ◽  
Hong-Xu Chen ◽  
Ying-Lin Zhou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Electrospray Ionization ◽  
Species Identification ◽  
Snake Venom ◽  
Electrospray Ionization Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Capillary Electrophoresis Mass Spectrometry

The first application of sheathless capillary electrophoresis-mass spectrometry combined with BLAST tools for snake characteristic peptide screening and species identification.

Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis–mass spectrometry

2016 ◽  
Vol 52 (13) ◽  
pp. 2698-2700 ◽  
Author(s):  
Fang Yuan ◽  
Xiao-Hui Zhang ◽  
Ji Nie ◽  
Hong-Xu Chen ◽  
Ying-Lin Zhou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Genomic Dna ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Mass Spectrometry System ◽  
Capillary Electrophoresis Mass Spectrometry

An ultrasensitive sheathless capillary electrophoresis–electrospray ionization–mass spectrometry system is developed for the determination of 5′-mdC and 5′-hmdC in only 125 pg (∼20 cells) genomic DNA samples.

scholarly journals Analysis of Inositol Phosphate Metabolism by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS)

2020 ◽  
Author(s):  
Danye Qiu ◽  
Miranda S. Wilson ◽  
Verena B. Eisenbeis ◽  
Robert K. Harmel ◽  
Esther Riemer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Inositol Phosphate ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Phosphate Metabolism ◽  
Esi Ms ◽  
Inositol Phosphate Metabolism

AbstractThe analysis of myo-inositol phosphates (InsPs) and myo-inositol pyrophosphates (PP-InsPs) is a daunting challenge due to the large number of possible isomers, the absence of a chromophore, the high charge density, the low abundance, and the instability of the esters and anhydrides. Given their importance in biology, an analytical approach to follow and understand this complex signaling hub is highly desirable. Here, capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry (ESI-MS) is implemented to analyze complex mixtures of InsPs and PP-InsPs with high sensitivity. Stable isotope labeled (SIL) internal standards allow for matrix-independent quantitative assignment. The method is validated in wild-type and knockout mammalian cell lines and in model organisms. SIL-CE-ESI-MS enables for the first time the accurate monitoring of InsPs and PP-InsPs arising from compartmentalized cellular synthesis pathways, by feeding cells with either [13C6]-myo-inositol or [13C6]-D-glucose. In doing so, we uncover that there must be unknown inositol synthesis pathways in mammals, highlighting the unique potential of this method to dissect inositol phosphate metabolism and signalling.

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