Volumetric dried blood spot sampling by coordinated burst action of hydrophobic burst valves

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Dries Vloemans ◽  
Lorenz Van Hileghem ◽  
Wannes Verbist ◽  
Debby Thomas ◽  
Francesco Dal Dosso ◽  
...  
Keyword(s):  
Dried Blood Spot ◽  
Sample Collection ◽  
Collection Method ◽  
Blood Spot

Dried blood spot (DBS) sampling by finger-pricking has recently gained a lot of interest as alternative sample collection method. The reduced invasiveness, requirement of lower sample volumes and suitability for...

scholarly journals Validation and Clinical Evaluation of a Novel Method To Measure Miltefosine in Leishmaniasis Patients Using Dried Blood Spot Sample Collection

2016 ◽  
Vol 60 (4) ◽  
pp. 2081-2089 ◽  
Author(s):  
A. E. Kip ◽  
H. Rosing ◽  
M. J. X. Hillebrand ◽  
S. Blesson ◽  
B. Mengesha ◽  
...  
Keyword(s):  
Venous Blood ◽  
Dried Blood Spot ◽  
Patient Specific ◽  
Pharmacokinetic Studies ◽  
Sample Collection ◽  
Limit Of Quantification ◽  
Simple Method ◽  
Linear Effect ◽  
Combination Treatments ◽  
Blood Spot

ABSTRACTTo facilitate future pharmacokinetic studies of combination treatments against leishmaniasis in remote regions in which the disease is endemic, a simple cheap sampling method is required for miltefosine quantification. The aims of this study were to validate a liquid chromatography-tandem mass spectrometry method to quantify miltefosine in dried blood spot (DBS) samples and to validate its use with Ethiopian patients with visceral leishmaniasis (VL). Since hematocrit (Ht) levels are typically severely decreased in VL patients, returning to normal during treatment, the method was evaluated over a range of clinically relevant Ht values. Miltefosine was extracted from DBS samples using a simple method of pretreatment with methanol, resulting in >97% recovery. The method was validated over a calibration range of 10 to 2,000 ng/ml, and accuracy and precision were within ±11.2% and ≤7.0% (≤19.1% at the lower limit of quantification), respectively. The method was accurate and precise for blood spot volumes between 10 and 30 μl and for Ht levels of 20 to 35%, although a linear effect of Ht levels on miltefosine quantification was observed in the bioanalytical validation. DBS samples were stable for at least 162 days at 37°C. Clinical validation of the method using paired DBS and plasma samples from 16 VL patients showed a median observed DBS/plasma miltefosine concentration ratio of 0.99, with good correlation (Pearson'sr= 0.946). Correcting for patient-specific Ht levels did not further improve the concordance between the sampling methods. This successfully validated method to quantify miltefosine in DBS samples was demonstrated to be a valid and practical alternative to venous blood sampling that can be applied in future miltefosine pharmacokinetic studies with leishmaniasis patients, without Ht correction.

scholarly journals Dried blood spot and mini-tube blood sample collection kits for postal HIV testing services: a comparative review of successes in a real-world setting

2018 ◽  
Vol 95 (1) ◽  
pp. 43-45 ◽  
Author(s):  
Matthew Page ◽  
Sowsan F Atabani ◽  
Martyn Wood ◽  
Erasmus Smit ◽  
Steven Wilson ◽  
...  
Keyword(s):  
Blood Sample ◽  
False Positive ◽  
Community Setting ◽  
Dried Blood Spot ◽  
Fisher Exact Test ◽  
Sample Collection ◽  
Transmitted Infection ◽  
North West ◽  
Comparative Review ◽  
Blood Spot

ObjectivesThis is a comparative review between using dried blood spot (DBS) and mini-tube (MT) HIV sampling kits as part of an online sexually transmitted infection (STI) postal testing service. England has recently seen increases in internet-based and postal (eHealth) STI services. Expanding accessibility and testing for patients, cost implications and narrowing the HIV undiagnosed margin are drivers for this.MethodsIn 2017, data were reviewed from an online postal STI kit requesting service at a time of transitioning from MT to DBS. We compared the STI postal kit and HIV blood sample return rates, and the successful processing/analysis rates of the DBS and MT kits. Descriptive statistics were applied to participant characteristics, with Pearson’s χ2 or Fisher exact test used to demonstrate statistical differences. We also describe and calculate a ‘request-to-result ratio’ (RRR) for both kit types. The RRR is defined as the number of online kit requests required to produce one successfully analysed result.Results550 STI postal kit requests from a North-West of England region were reviewed from 13 June 2017 to 22 September 2017 (275 MT, 275 DBS). Baseline characteristics between the two groups were comparable (63% woman, 90% white British and 86% heterosexual with a median age of 26 years). The successful processing rate for the DBS was 98.8% c.f. 55.7% for the MT (p<0.001). The RRR for MT was 2.96, c.f. 1.70 for DBS. There was a 5.4% false positive HIV rate in the MT c.f. none in the DBS.ConclusionsThis comparative analysis suggests that in this community setting, the use of postal HIV DBS kits resulted in a significantly improved RRR compared with MT. The biggest factor was the large number of MT samples not analysed due to inadequate blood volumes. The unexpected level of false positive results in the MT samples needs confirming in larger studies.

Perforated dried blood spot accurate microsampling: the concept and its applications in toxicokinetic sample collection

2012 ◽  
Vol 47 (5) ◽  
pp. i-i
Author(s):  
Fumin Li ◽  
Stephen Ploch ◽  
Douglas M. Fast ◽  
Steven M. Michael
Keyword(s):  
Dried Blood Spot ◽  
Sample Collection ◽  
Blood Spot

scholarly journals Detection of ctDNA from dried blood spots after DNA size selection

2019 ◽  
Author(s):  
Katrin Heider ◽  
Jonathan C. M. Wan ◽  
James Hall ◽  
Samantha Boyle ◽  
Irena Hudecova ◽  
...  
Keyword(s):  
Copy Number ◽  
Fragment Size ◽  
Dried Blood Spots ◽  
Dried Blood Spot ◽  
Copy Number Alterations ◽  
Sample Collection ◽  
Cell Free Dna ◽  
Blood Spots ◽  
Free Dna ◽  
Blood Spot

AbstractRecent advances in the research and clinical applications of circulating tumour DNA (ctDNA) is limited by practical considerations of sample collection. Whole genome sequencing (WGS) is increasingly used for analysis of ctDNA, identifying copy-number alterations, fragment size patterns, and other genomic features. We hypothesised that low-depth WGS data may be generated from minute amounts of cell-free DNA, and that fragment-size selection may be effective to remove contaminating genomic DNA (gDNA) from small volumes of blood. There are practical advantages to using dried blood spots as these are easier to collect, facilitate serial sampling, and support novel study designs in prospective human studies, animal models and expand the utilisation of archival samples by the removal of gDNA in small volumes. We therefore developed a protocol for the isolation and analysis of cell-free DNA from dried blood spots. Analysing a dried blood spot of 50μL frozen whole blood from a patient with melanoma, we identified ctDNA based on tumour-specific somatic copy-number alterations, and found a fragment size profile similar to that observed in plasma DNA processed by traditional methods. We extended this approach to detect tumour-derived cell-free DNA in a dried blood spot from a mouse xenograft model and were able to identify ctDNA from the originally grafted ascites. Together, our data suggests that ctDNA can be detected and monitored in dried blood spots. This will enable new approaches for sample collection from patients andin vivomodels.

scholarly journals Metabolic profiles derived from residual blood spot samples: A longitudinal analysis

2018 ◽  
Vol 2 ◽  
pp. 28 ◽  
Author(s):  
Malia S.Q. Murphy ◽  
Steven Hawken ◽  
Wei Cheng ◽  
Lindsay A. Wilson ◽  
Monica Lamoureux ◽  
...  
Keyword(s):  
Newborn Screening ◽  
Gestational Age ◽  
High Performance ◽  
Intraclass Correlation ◽  
Estimation Algorithm ◽  
Dried Blood Spot ◽  
Sample Collection ◽  
Secondary Use ◽  
Blood Spot ◽  
Age Estimates

Background: Secondary use of newborn screening dried blood spot samples include use for biomedical or epidemiological research. However, the effects of storage conditions on archival samples requires further examination. The objective of this study was to determine the utility of residual newborn samples for deriving reliable metabolic gestational age estimates. Methods: Residual newborn dried blood spot samples that had been stored for 2-, 4-, 6-, or 12-months in temperature controlled (21°C) conditions were re-analyzed for the full panel of newborn screening analytes offered by a provincial newborn screening lab in Ottawa, Canada. Data from re-analyzed samples were compared to corresponding baseline newborn screening values for absolute agreement, and Pearson and intraclass correlation. Performance of a gestational age estimation algorithm originally developed from baseline newborn screening values was then validated on data derived from stored samples. Results: A total of 307 samples were used for this study. 17-hydroxyprogesterone and newborn hemoglobin profiles measured by immunoassay and high-performance liquid chromatography, respectively, were among the most stable markers across all time points of analysis. Acylcarnitines exhibited the greatest degree of variation in stability upon repeat measurement. The largest shifts in newborn analyte profiles and the poorest performance of metabolic gestational age algorithms were observed when samples were analyzed 12-months after sample collection. Conclusions: Duration of sample storage, independent of temperature and humidity, affects newborn screening profiles and gestational age estimates derived from metabolic gestational dating algorithms. When considering use of dried blood spot samples either for clinical or research purposes, care should be taken when interpreting data stemming from secondary use.

scholarly journals Are all blood-based postal sampling kits the same? A comparative service evaluation of the performance of dried blood spot and mini tube sample collection systems for postal HIV and syphilis testing

2020 ◽  
pp. sextrans-2020-054692
Author(s):  
Matthew Page ◽  
Sowsan Atabani ◽  
Joseph Arumainayagam ◽  
Steven Wilson ◽  
Daniel Hartland ◽  
...  
Keyword(s):  
False Positive ◽  
Dried Blood Spot ◽  
Blood Collection ◽  
Sample Collection ◽  
Sample Return ◽  
Return Rates ◽  
Percentage Difference ◽  
Baseline Characteristics ◽  
Positive Results ◽  
Blood Spot

ObjectivesWe comparatively evaluated two HIV and syphilis blood sampling kits (dried blood spot (DBS) and mini tube (MT)) as part of an online STI postal sampling service that included tests for chlamydia and gonorrhoea. We aimed to see how the blood collection systems compared regarding sample return rates and result rates. Additionally, we aimed to observe differences in false-positive results and describe a request-to-result ratio (RRR)—the required number of kit requests needed to obtain one successful result.MethodsWe reviewed data from an online postal STI kit requesting service for a client transitioning from MT to DBS blood collection systems. We described service user baseline characteristics and compared kit requests, kit and blood sample return rates, and the successful resulting rates for HIV and syphilis for MT and DBS. Pearson’s χ2 and Fisher’s exact test were used to determine statistical differences, and statistical formulae were applied to produce CIs for differences in proportions.Results5670 STI postal kit requests from a Midlands region were reviewed from 6 September 2016–2 January 2019 (1515 MT and 4155 DBS). Baseline characteristics between the two groups were comparable (68.0% female, 74.0% white British and 87.5% heterosexual, median age 26 years). Successful processing rates for DBS were 94.6% and 54.4% for MT (p<0.001) with a percentage difference of 40.2% (95% CI 36.9% to 43.4%). The RRR for MT was 2.9 cf. 1.6 for DBS. False-positive results for MT samples were 5.2% (HIV) and 0.4% (syphilis), and those for DBS were 0.4% (HIV) and 0.0% (syphilis).ConclusionsThis comparative analysis demonstrated the superior successful processing rates for postal DBS collection systems compared with MT. Reasons for this included insufficient volumes, high false-positive rates and degradation of blood quality in MT samples. A postal sampling service using DBS to screen for HIV, syphilis and other blood-borne viruses could be a viable alternative.

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