Membrane proteins in nanotechnology

The potential for nanotechnology to transform modern life has been appreciated for several years. Generally, nanotechnology involves objects with at least one dimension of less than 100 nm. Such stuff is abundant in the natural world, and one of the most exciting current areas in nanoscience research is the use of proteins as nanoscale components of high-performance devices and as nanosized tools in their own right. Biochemists, with our relatively sophisticated and mature toolkit for understanding protein structure and function, are now saddling up to explore the wild frontiers of biological nanotechnology.

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Nanoscale lipid membrane mimetics in spin-labeling and electron paramagnetic resonance spectroscopy studies of protein structure and function

Nanotechnology Reviews ◽

10.1515/ntrev-2016-0080 ◽

2017 ◽

Vol 6 (1) ◽

pp. 75-92 ◽

Cited By ~ 6

Author(s):

Elka R. Georgieva

Keyword(s):

Electron Paramagnetic Resonance ◽

Protein Structure ◽

Membrane Proteins ◽

Membrane Protein ◽

Spin Labeling ◽

Structure And Function ◽

Protein Structure And Function ◽

Paramagnetic Resonance ◽

And Function ◽

Electron Paramagnetic

AbstractCellular membranes and associated proteins play critical physiological roles in organisms from all life kingdoms. In many cases, malfunction of biological membranes triggered by changes in the lipid bilayer properties or membrane protein functional abnormalities lead to severe diseases. To understand in detail the processes that govern the life of cells and to control diseases, one of the major tasks in biological sciences is to learn how the membrane proteins function. To do so, a variety of biochemical and biophysical approaches have been used in molecular studies of membrane protein structure and function on the nanoscale. This review focuses on electron paramagnetic resonance with site-directed nitroxide spin-labeling (SDSL EPR), which is a rapidly expanding and powerful technique reporting on the local protein/spin-label dynamics and on large functionally important structural rearrangements. On the other hand, adequate to nanoscale study membrane mimetics have been developed and used in conjunction with SDSL EPR. Primarily, these mimetics include various liposomes, bicelles, and nanodiscs. This review provides a basic description of the EPR methods, continuous-wave and pulse, applied to spin-labeled proteins, and highlights several representative applications of EPR to liposome-, bicelle-, or nanodisc-reconstituted membrane proteins.

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High-Performance Deep Learning Toolbox for Genome-Scale Prediction of Protein Structure and Function

10.1109/mlhpc54614.2021.00010 ◽

2021 ◽

Author(s):

Mu Gao ◽

Peik Lund-Andersen ◽

Alex Morehead ◽

Sajid Mahmud ◽

Chen Chen ◽

...

Keyword(s):

Deep Learning ◽

Protein Structure ◽

High Performance ◽

Structure And Function ◽

Protein Structure And Function ◽

And Function ◽

Genome Scale

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Faculty Opinions recommendation of A minimal sequence code for switching protein structure and function.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1367956.838071 ◽

2010 ◽

Author(s):

H Jane Dyson ◽

Gira Bhabha

Keyword(s):

Protein Structure ◽

Structure And Function ◽

Protein Structure And Function ◽

Sequence Code ◽

Minimal Sequence ◽

And Function

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Faculty Opinions recommendation of A minimal sequence code for switching protein structure and function.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1367956.1868054 ◽

2010 ◽

Author(s):

Nick Grishin

Keyword(s):

Protein Structure ◽

Structure And Function ◽

Protein Structure And Function ◽

Sequence Code ◽

Minimal Sequence ◽

And Function

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Faculty Opinions recommendation of Small-molecule structure correctors target abnormal protein structure and function: structure corrector rescue of apolipoprotein E4-associated neuropathology.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717959324.793462720 ◽

2012 ◽

Author(s):

John Lowe

Keyword(s):

Protein Structure ◽

Small Molecule ◽

Structure And Function ◽

Function Structure ◽

Protein Structure And Function ◽

Abnormal Protein ◽

Apolipoprotein E4 ◽

Molecule Structure ◽

And Function

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Order-disorder transitions of cytoplasmic N-termini in the mechanisms of P-type ATPases

Faraday Discussions ◽

10.1039/d0fd00040j ◽

2020 ◽

Author(s):

Khondker Rufaka Hossain ◽

Daniel Clayton ◽

Sophia C Goodchild ◽

Alison Rodger ◽

Richard James Payne ◽

...

Keyword(s):

Protein Structure ◽

Membrane Protein ◽

Structure And Function ◽

Membrane Protein Structure ◽

Protein Structure And Function ◽

Lipid Environment ◽

Membrane Pumps ◽

And Function ◽

P Type

Membrane protein structure and function are modulated via interactions with their lipid environment. This is particularly true for the integral membrane pumps, the P-type ATPases. These ATPases play vital roles...

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