scholarly journals The monophenolic metabolites of the herbicide 2,6-dichlorobenzonitrile in animals as uncouplers of oxidative phosphorylation

1966 ◽  
Vol 101 (3) ◽  
pp. 707-710 ◽  
Author(s):  
JG Wit ◽  
H Van Genderen
Keyword(s):  
Oxygen Consumption ◽  
Oxidative Phosphorylation ◽  
Rat Liver ◽  
Adenosine Triphosphatase ◽  
Hepatic Injury ◽  
Liver Mitochondria ◽  
Significant Rise ◽  
Yeast Cells ◽  
Rat Liver Mitochondria

1. Both monophenolic metabolites of 2,6-dichlorobenzonitrile (2,6-dichloro-3-hydroxybenzonitrile and its 4-hydroxy analogue) added to starved yeast cells incubated with a limited quantity of glucose cause a significant rise in oxygen consumption of the cells. 2. The same compounds induce adenosine-triphosphatase activity in isolated intact rat-liver mitochondria. 3. The possible role of the hydroxylation of 2,6-dichlorobenzonitrile in mammals in relation to hepatic injury is discussed.

Oxidative phosphorylation and adenosine triphosphatase activity in the liver mitochondria of rats administered with phenylhydrazine and hydroiyzed glucose cycloacetoacetate

1969 ◽  
Vol 47 (3) ◽  
pp. 297-300 ◽  
Author(s):  
T. G. Reddi ◽  
M. C. Nath
Keyword(s):  
Oxygen Uptake ◽  
Oxidative Phosphorylation ◽  
Rat Liver ◽  
Adenosine Triphosphatase ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Partial Restoration ◽  
Atp Formation

The effect of phenylhydrazine and hydroiyzed product of glucose cycloacetoacetate (GCAh) administration on the activity of adenosine triphosphatase (ATPase) in rat-liver mitochondria has been investigated. The results are discussed in relation to the efficiency of mitochondrial oxidative phosphorylation. Phenylhydrazine was found to increase the ATPase activity both in vitro and in vivo. However, preincubation or treatment with hydrolyzed glucose cycloacetoacetate resulted in an appreciable depression of this phenylhydrazine-enhanced enzyme activity. The liver mitochondria from phenylhydrazine-administered rats showed very little difference in milligrams of total protein, but the homogenates had a high protein content as compared to the preparations from normal rats and rats administered with hydrolyzed glucose cycloacetoacetate. With citrate as the substrate, normal rat-liver mitochondria exhibited a P/O ratio of 3.0. With the same substrate, the liver mitochondria from phenylhydrazine-administered rats lowered the oxygen uptake and ATP formation, thereby resulting in a decreased P/O ratio of 2.4, whereas administration of hydrolyzed glucose cycloacetoacetate prior to phenylhydrazine resulted in a partial restoration in oxygen uptake and ATP formation, and thus yielded a P/O ratio of 2.8.

The role of sulfhydryl groups in oxidative phosphorylation and ion transport by rat liver mitochondria

1969 ◽  
Vol 172 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Niels Haugaard ◽  
Nam Hea Lee ◽  
Richard Kostrzewa ◽  
Robert S. Horn ◽  
Ella S. Haugaard
Keyword(s):  
Oxidative Phosphorylation ◽  
Ion Transport ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Sulfhydryl Groups ◽  
Rat Liver Mitochondria
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